FastQCFastQC Report
Fri 10 Feb 2017
SRR2568612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418068
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.37912492704536105No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC15510.3709922787680473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.28464268970598083No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG10880.26024474487403965No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10360.24780657692050098No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC10060.24063071079345943No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG10010.2394347331056192No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT9450.22603978300180833No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA9390.22460460977640004No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG9330.22316943655099172No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9310.22269104547585558No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC9110.21790713472449458No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG8930.2136016150482697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.20475138015825178No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT8510.2035554024704115No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT8150.19494436311796168No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC7930.18968206129146456No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT7790.18633332376551182No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT7610.1820278040892869No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG7610.1820278040892869No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7470.1786790665633342No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT7410.1772438933379259No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7380.17652630672522174No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7340.1755695245749495No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC7270.17389515581197318No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7200.17222078704899682No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT7070.16911124506061215No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT6840.16360974769654696No Hit
GTGTTATGGCTGGGGGTTTCATGTTGATAATAGTGGTAATAAAATTAATT6600.15786905479491375No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG6520.15595549049436935No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6470.15475951280652908No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG6390.15284594850598468No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC6330.15141077528057637No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG6220.1487796243673278No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6200.1483012332921917No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG6060.144952495766239No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA5910.14136456270271822No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5850.13992938947730993No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT5730.1370590430264933No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC5710.13658065195135718No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT5690.1361022608762211No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT5610.1341886965756767No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5530.13227513227513227No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT5370.12844800367404346No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG5350.12796961259890738No Hit
ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA5320.1272520259862032No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG5120.12246811523484219No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG5110.12222891969727412No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC5100.12198972415970606No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5060.12103294200943386No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT4970.11888018217132142No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT4860.11624903125807284No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA4800.11481385803266454No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC4790.11457466249509649No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC4790.11457466249509649No Hit
GTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTC4780.11433546695752844No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT4770.11409627141996039No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG4760.11385707588239234No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG4750.11361788034482428No Hit
ATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCT4660.11146512050671184No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4630.11074753389400767No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4630.11074753389400767No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA4620.11050833835643961No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT4580.10955155620616741No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA4490.10739879636805497No Hit
GTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTACTGC4430.10596362314264664No Hit
ATCAATGGGAGCAGTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCC4420.1057244276050786No Hit
GCCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTAC4300.10285408115426199No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC4260.10189729900398979No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC4230.10117971239128563No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT4230.10117971239128563No Hit
GTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACG4220.10094051685371759No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC4200.10046212577858146No Hit
CCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACA4200.10046212577858146No Hit
GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT4190.10022293024101342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTCG200.002070709471.271149
GACATAT1350.070.391241
CATTCCG353.407191E-667.8772669
ACATATA1400.064.4834062
CCTTTAG452.0402877E-763.352121
GAGTATG407.523833E-659.3926161
AGGGTCT407.523833E-659.3926165
AGTATGG407.523833E-659.3926162
GGCATTC451.5116326E-552.7934347
CTTTAGG451.5116326E-552.7934342
GTCTTCT451.5116326E-552.7934348
CATATAG1850.048.7982563
GAGAGCC405.3763384E-447.5140958
CCTCATC652.5801164E-643.859163
TGGGGAG704.294814E-640.726366
ATGGGCG500.00161228438.0112725
TGGGCGT500.00161228438.0112726
GGATATG3650.037.7509231
GGATGAC3600.036.9554028
TATGGGC651.3244202E-436.54932
Produced by FastQC (version 0.11.5)