##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568612_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418068 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44883607451419 34.0 31.0 34.0 31.0 34.0 2 32.56612799831606 34.0 33.0 34.0 31.0 34.0 3 32.67103916109341 34.0 33.0 34.0 31.0 34.0 4 35.85206234392491 37.0 37.0 37.0 35.0 37.0 5 35.8866452347465 37.0 37.0 37.0 35.0 37.0 6 36.01461963125616 37.0 37.0 37.0 35.0 37.0 7 36.04283274491231 37.0 37.0 37.0 35.0 37.0 8 36.08420878900083 37.0 37.0 37.0 35.0 37.0 9 37.91854674359195 39.0 39.0 39.0 37.0 39.0 10-11 37.937737401571034 39.0 39.0 39.0 37.0 39.0 12-13 37.93976099581886 39.0 39.0 39.0 37.0 39.0 14-15 39.46669919725977 41.0 40.0 41.0 38.0 41.0 16-17 39.45100677401763 41.0 40.0 41.0 38.0 41.0 18-19 39.54185802309672 41.0 40.0 41.0 38.0 41.0 20-21 39.564282604743724 41.0 40.0 41.0 38.0 41.0 22-23 39.50391682692768 41.0 40.0 41.0 38.0 41.0 24-25 39.43668972511649 41.0 40.0 41.0 37.0 41.0 26-27 39.341884573801394 41.0 40.0 41.0 37.0 41.0 28-29 39.28095549049437 41.0 40.0 41.0 37.0 41.0 30-31 39.157704009873996 41.0 40.0 41.0 36.0 41.0 32-33 39.00245414621545 41.0 39.0 41.0 35.0 41.0 34-35 38.94254762383153 41.0 39.0 41.0 35.0 41.0 36-37 38.817978175799155 41.0 39.0 41.0 35.0 41.0 38-39 38.733686864337855 41.0 39.0 41.0 35.0 41.0 40-41 38.628485078982365 41.0 39.0 41.0 35.0 41.0 42-43 38.540967019719275 40.5 39.0 41.0 35.0 41.0 44-45 38.373286163973326 40.0 38.5 41.0 35.0 41.0 46-47 38.31234272893405 40.5 38.5 41.0 35.0 41.0 48-49 38.36960494465015 41.0 39.0 41.0 35.0 41.0 50-51 36.95872441803726 39.5 37.0 40.0 32.5 40.5 52-53 37.184710382043114 39.5 37.5 40.0 33.5 40.5 54-55 38.035937933541916 40.0 39.0 41.0 35.0 41.0 56-57 38.05570624874423 41.0 39.0 41.0 34.5 41.0 58-59 37.932257431805354 40.5 39.0 41.0 34.0 41.0 60-61 37.71901221810806 40.0 38.0 41.0 33.5 41.0 62-63 37.4704043361367 40.0 38.0 41.0 33.0 41.0 64-65 37.22918161638776 40.0 37.0 41.0 33.0 41.0 66-67 37.128101170144575 39.5 37.0 41.0 33.0 41.0 68-69 36.79462431948869 39.0 36.5 41.0 33.0 41.0 70-71 36.4167025459973 39.0 36.0 41.0 32.5 41.0 72-73 36.020084053311905 37.5 35.5 40.0 32.0 41.0 74-75 35.53790172890534 37.0 35.0 39.0 31.5 41.0 76-77 35.12635384674263 36.5 35.0 39.0 31.0 41.0 78-79 34.71877063061511 36.0 35.0 38.0 31.0 39.5 80-81 34.32148358640221 36.0 35.0 37.0 31.0 39.0 82-83 33.95311887061435 35.0 35.0 37.0 30.5 39.0 84-85 33.587251356238696 35.0 35.0 36.5 30.0 38.0 86-87 33.38252509161189 35.0 35.0 36.0 30.0 37.0 88-89 33.167130706009544 35.0 35.0 36.0 29.0 37.0 90-91 32.986591894141625 35.0 35.0 36.0 29.0 36.5 92-93 32.858220433039605 35.0 34.5 35.0 29.0 36.0 94-95 32.73478955576605 35.0 34.0 35.0 29.0 36.0 96-97 32.63112460173943 35.0 34.0 35.0 29.0 36.0 98-99 32.557039763866165 35.0 34.0 35.0 28.0 36.0 100-101 31.496391735315783 34.5 32.0 35.0 23.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023219288074827205 1101 2 -0.09220646338312832 1101 3 -0.01163575129857719 1101 4 -0.24306988266299356 1101 5 -0.08268220410540295 1101 6 -0.0579198585952625 1101 7 -0.05031550305587018 1101 8 0.07234546876262016 1101 9 0.007553438503030918 1101 10-11 -0.10254590278267273 1101 12-13 0.03885679698832689 1101 14-15 0.08366970469830903 1101 16-17 0.19859400146724226 1101 18-19 0.05762770657679539 1101 20-21 0.02386458789200674 1101 22-23 0.22609692076208177 1101 24-25 0.17004741512783283 1101 26-27 0.21374985649426748 1101 28-29 0.22827030086687472 1101 30-31 0.24819429287376238 1101 32-33 0.1326414451309006 1101 34-35 0.15586314889754505 1101 36-37 0.19483799314148342 1101 38-39 0.0568581087193607 1101 40-41 0.10647778825377685 1101 42-43 0.09101796929543582 1101 44-45 0.10983630773645103 1101 46-47 0.08081973235342588 1101 48-49 0.2425347685657755 1101 50-51 0.05796197343924092 1101 52-53 0.06036161237132376 1101 54-55 0.1915139285647527 1101 56-57 0.03380972326646514 1101 58-59 0.0365976492959561 1101 60-61 -0.10932393394286777 1101 62-63 -0.14634878494296544 1101 64-65 -0.06281972586530316 1101 66-67 0.10566635440429906 1101 68-69 -0.005833500900280342 1101 70-71 0.06429835951317386 1101 72-73 0.14460549658937083 1101 74-75 0.011704751728515816 1101 76-77 0.06915585578376948 1101 78-79 0.12097078549476237 1101 80-81 0.0399617564317154 1101 82-83 0.09381358035744825 1101 84-85 0.08571922200483328 1101 86-87 0.03146593185353197 1101 88-89 0.05761706264965483 1101 90-91 0.23658449256677017 1101 92-93 -0.00625222263588654 1101 94-95 -0.12909723628545322 1101 96-97 0.031803482831605834 1101 98-99 -0.0803524140204388 1101 100-101 0.22773409216659957 1102 1 -0.17004148619935222 1102 2 -0.02140075938304875 1102 3 0.022741201407470157 1102 4 -0.030466243518411318 1102 5 -0.1085441926712889 1102 6 -0.08461189295356064 1102 7 0.053477526424742905 1102 8 -0.0685384833745104 1102 9 0.08132492409847458 1102 10-11 0.2407153378093696 1102 12-13 0.2380817767035026 1102 14-15 0.18489798594394102 1102 16-17 0.3245044870906568 1102 18-19 0.3475095416308349 1102 20-21 0.22744497642805328 1102 22-23 0.3429468503480848 1102 24-25 0.2553649309593169 1102 26-27 0.3556783606398284 1102 28-29 0.32579135511829094 1102 30-31 0.21999138864612888 1102 32-33 0.058217114530187075 1102 34-35 0.27553896472064565 1102 36-37 0.12215302816196072 1102 38-39 0.06482281778365007 1102 40-41 0.1576002153449778 1102 42-43 0.2905693715132429 1102 44-45 0.08197381554727201 1102 46-47 0.18192210777660733 1102 48-49 0.3595525725817055 1102 50-51 0.36014061048539503 1102 52-53 0.4105336836619955 1102 54-55 0.7082314238782601 1102 56-57 0.43714669387912153 1102 58-59 0.5029714683459687 1102 60-61 0.24602426560387158 1102 62-63 0.2789144514895199 1102 64-65 0.38122679400449755 1102 66-67 0.272440274178976 1102 68-69 0.14897470256352818 1102 70-71 0.13264656838627076 1102 72-73 0.07062883113513152 1102 74-75 -0.07185940894596854 1102 76-77 -0.16688958222859895 1102 78-79 0.018632667740170916 1102 80-81 -0.07188458250676177 1102 82-83 -0.1520431854483988 1102 84-85 -0.17378200441113734 1102 86-87 -0.14346388730839976 1102 88-89 -0.07640735375354524 1102 90-91 0.011427576606578782 1102 92-93 0.14235395327786193 1102 94-95 -0.0032333824481298734 1102 96-97 -0.15456511345836077 1102 98-99 -0.20867842616335963 1102 100-101 -0.3227168372048972 1103 1 0.2253048688011816 1103 2 0.07547902145446272 1103 3 0.20660109396092707 1103 4 0.11938966426257025 1103 5 0.1777456995263904 1103 6 0.22692971589832212 1103 7 0.18614020831893896 1103 8 0.22753544838625572 1103 9 0.16999500628440956 1103 10-11 0.2857886291602725 1103 12-13 0.3609381825810445 1103 14-15 0.35518474724894844 1103 16-17 0.2770430006282112 1103 18-19 0.16986251419995568 1103 20-21 0.15066250231128464 1103 22-23 0.3753987306625959 1103 24-25 0.2965157119035666 1103 26-27 0.20609373759219096 1103 28-29 0.17532172121926237 1103 30-31 0.3882368435150241 1103 32-33 0.41136312797879526 1103 34-35 0.3143193397161994 1103 36-37 0.27916017940542304 1103 38-39 0.30892571030152993 1103 40-41 0.3063309585762042 1103 42-43 0.22106711228956044 1103 44-45 0.3059438230187226 1103 46-47 0.30618830480937476 1103 48-49 0.25480553447732035 1103 50-51 0.2615308335615012 1103 52-53 0.3482226935171866 1103 54-55 0.3636642342102121 1103 56-57 0.41655573788950306 1103 58-59 0.5398039300103221 1103 60-61 0.5069112332631747 1103 62-63 0.49379864403941554 1103 64-65 0.4710709609380004 1103 66-67 0.5627860955046202 1103 68-69 0.499590207597862 1103 70-71 0.39854563267630994 1103 72-73 0.3439192925860155 1103 74-75 0.2938724372133521 1103 76-77 0.3123237809905248 1103 78-79 0.305451787532391 1103 80-81 0.333036789393482 1103 82-83 0.456452918724942 1103 84-85 0.4966025972895025 1103 86-87 0.21608177747261692 1103 88-89 0.1368002101985013 1103 90-91 0.471482011444877 1103 92-93 0.2943890335069881 1103 94-95 0.1804886440933089 1103 96-97 0.2588950566060291 1103 98-99 0.28137298451726167 1103 100-101 0.06309007922161314 1104 1 -0.022471783230791686 1104 2 0.09555482167210982 1104 3 0.13814636770477762 1104 4 0.03732279496166058 1104 5 0.17619779904231336 1104 6 0.010129835977878088 1104 7 0.12136291836434054 1104 8 0.182909008369748 1104 9 0.1256124778593275 1104 10-11 0.22853976428729794 1104 12-13 0.10361614423064225 1104 14-15 0.26440272916196506 1104 16-17 0.29248448060869947 1104 18-19 0.31340917636363486 1104 20-21 0.32916357176979716 1104 22-23 0.27168470762233454 1104 24-25 0.2646805586145078 1104 26-27 0.16367657372112632 1104 28-29 0.31831958230223023 1104 30-31 0.17878714559014952 1104 32-33 0.2721787308156749 1104 34-35 0.24589275710521719 1104 36-37 0.13585273822563693 1104 38-39 0.3685902380511621 1104 40-41 0.4844004921421927 1104 42-43 0.2952162360840731 1104 44-45 0.21890960034620122 1104 46-47 0.06823378493996302 1104 48-49 0.007380678009909047 1104 50-51 -0.19246837841644293 1104 52-53 -0.171585742071386 1104 54-55 -0.21313977344486545 1104 56-57 -0.14750857972091325 1104 58-59 -0.22970899730401584 1104 60-61 -0.40806681469359773 1104 62-63 -0.3868441568161529 1104 64-65 -0.449423241471095 1104 66-67 -0.28295858534644225 1104 68-69 -0.15929943178822725 1104 70-71 -0.3671256870730346 1104 72-73 -0.2719996912407723 1104 74-75 -0.3503121475740656 1104 76-77 -0.33799142783531977 1104 78-79 -0.11045031010729645 1104 80-81 -0.02860436036823444 1104 82-83 -0.012589071593644974 1104 84-85 -0.09360716667912072 1104 86-87 -0.19977810469932678 1104 88-89 -0.4675892603257239 1104 90-91 -0.38304348669764465 1104 92-93 -0.35009105955466424 1104 94-95 -0.4302894338833525 1104 96-97 -0.41063757125993305 1104 98-99 -0.21277782789596245 1104 100-101 -0.3065907718422203 1105 1 0.10515554175530184 1105 2 0.04368152988685381 1105 3 0.11660371373366019 1105 4 -0.026498929901883628 1105 5 0.004275810539255076 1105 6 0.056988333313263695 1105 7 0.14395368050022483 1105 8 -0.016046108413689808 1105 9 0.20877582954798868 1105 10-11 0.12226159638036194 1105 12-13 0.22808705116669614 1105 14-15 0.34777570248358103 1105 16-17 0.3763815080126989 1105 18-19 0.2839331498223103 1105 20-21 0.4689771697638818 1105 22-23 0.35950407827369446 1105 24-25 0.37974671036445073 1105 26-27 0.24634408962415222 1105 28-29 0.3437321741124748 1105 30-31 0.39530613650385504 1105 32-33 0.2633746222316731 1105 34-35 0.48134622513389047 1105 36-37 0.5102732553460783 1105 38-39 0.283923254264586 1105 40-41 0.5209189993136718 1105 42-43 0.33376646062109216 1105 44-45 0.5870495308485673 1105 46-47 0.4670832846698758 1105 48-49 0.5941438781260047 1105 50-51 0.41145960849026864 1105 52-53 0.48090190234582053 1105 54-55 0.5200401061033162 1105 56-57 0.5562960529171761 1105 58-59 0.7697790421693469 1105 60-61 0.7053344575484672 1105 62-63 0.7843723742683224 1105 64-65 0.744992760721864 1105 66-67 0.8180911352924198 1105 68-69 0.9735644028364305 1105 70-71 0.8927043581568697 1105 72-73 0.8647260188525152 1105 74-75 0.9052004982540609 1105 76-77 1.0284795919739267 1105 78-79 0.8796773172176984 1105 80-81 0.9580040422343004 1105 82-83 0.8926941997938087 1105 84-85 0.9430282448645855 1105 86-87 0.7779657215404683 1105 88-89 0.8568324661502942 1105 90-91 0.8124044989190864 1105 92-93 0.7401188039494002 1105 94-95 0.7469940310009875 1105 96-97 0.8805712199488624 1105 98-99 0.9922442226873471 1105 100-101 0.6609017503091472 1106 1 0.3813026576347909 1106 2 0.38154130264112496 1106 3 0.3828771212319779 1106 4 0.38506130605361477 1106 5 0.4549983252864749 1106 6 0.40328866190900925 1106 7 0.37155572333644216 1106 8 0.43738435201625947 1106 9 0.44365448666030716 1106 10-11 0.36060436529441375 1106 12-13 0.4888495516615663 1106 14-15 0.5265040765284752 1106 16-17 0.4095102937920245 1106 18-19 0.5465761681524128 1106 20-21 0.509465708502546 1106 22-23 0.4614637758072462 1106 24-25 0.45549396876739223 1106 26-27 0.35086525039852745 1106 28-29 0.42189441331434097 1106 30-31 0.40793925737166603 1106 32-33 0.40974621255590904 1106 34-35 0.386477069585375 1106 36-37 0.5077395049996767 1106 38-39 0.5169411884662551 1106 40-41 0.4233062672181802 1106 42-43 0.3179899057297675 1106 44-45 0.3626740607191081 1106 46-47 0.27863164367062865 1106 48-49 0.18469774011579432 1106 50-51 0.42868119287603434 1106 52-53 0.43587240790819237 1106 54-55 0.3607067991632178 1106 56-57 0.5324669227061563 1106 58-59 0.5252562978010005 1106 60-61 0.6207586627822437 1106 62-63 0.6310844372953568 1106 64-65 0.42792557674783893 1106 66-67 0.2799502746090994 1106 68-69 0.235696712112329 1106 70-71 0.3337907036178933 1106 72-73 0.20003224633578753 1106 74-75 0.2393625790965359 1106 76-77 0.2350158760706833 1106 78-79 0.10924301657237834 1106 80-81 0.365407344027723 1106 82-83 0.3188630900903391 1106 84-85 0.2667702774074385 1106 86-87 0.30921794846948103 1106 88-89 0.38588349746586204 1106 90-91 0.31833109933874226 1106 92-93 0.1607332384084046 1106 94-95 0.13308215800522305 1106 96-97 0.060364560936228884 1106 98-99 0.17818982623460045 1106 100-101 0.21420948220668734 1107 1 0.09047871611788594 1107 2 0.1337115737346366 1107 3 -0.0012913222648265332 1107 4 0.1806141540584889 1107 5 0.2468122150214711 1107 6 0.11710986203085127 1107 7 0.11001901910250922 1107 8 0.19213610346310617 1107 9 0.12615524815893053 1107 10-11 0.13616025929380982 1107 12-13 0.14521948769568382 1107 14-15 0.13606469181937086 1107 16-17 0.17293932211977392 1107 18-19 0.09125329273359029 1107 20-21 0.24552266860001737 1107 22-23 0.11938336155199636 1107 24-25 0.13824221732054554 1107 26-27 0.10228925283532675 1107 28-29 0.2177442457846439 1107 30-31 0.05134467924190744 1107 32-33 0.010427753829141295 1107 34-35 -0.025139592004634892 1107 36-37 -0.02904087879564088 1107 38-39 0.1111271464315351 1107 40-41 0.22381647129981275 1107 42-43 0.3069672130706138 1107 44-45 0.244908297697485 1107 46-47 0.46638293821829535 1107 48-49 0.3599642646360479 1107 50-51 0.10572962418886078 1107 52-53 0.13896028485001466 1107 54-55 0.15613399989425858 1107 56-57 0.21489914932815424 1107 58-59 0.14743494537943036 1107 60-61 0.04446792260557686 1107 62-63 0.16561459264107725 1107 64-65 0.03673611588886416 1107 66-67 0.18906160570870156 1107 68-69 0.11659071150312883 1107 70-71 0.12068911970074936 1107 72-73 -0.023600863624615442 1107 74-75 -0.02969773159490785 1107 76-77 0.014185081066109717 1107 78-79 0.061797844473687746 1107 80-81 0.11408995139902345 1107 82-83 0.1580307720885159 1107 84-85 0.06615727101084445 1107 86-87 0.16984846933454634 1107 88-89 0.21185060072507866 1107 90-91 0.28043892754464395 1107 92-93 0.2508642161726513 1107 94-95 0.36348499078012964 1107 96-97 0.2908930409849688 1107 98-99 0.11369850731897202 1107 100-101 0.13834518126242656 1108 1 0.23350592700224126 1108 2 0.08456191387068657 1108 3 0.12608963011612673 1108 4 0.04081823569114107 1108 5 0.10684622862691384 1108 6 0.16864047427574747 1108 7 0.20236595787802258 1108 8 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-0.7964537853990166 2316 50-51 -0.5803570854540823 2316 52-53 -0.8705569835958187 2316 54-55 -1.0221180292316276 2316 56-57 -1.1299729238873866 2316 58-59 -0.9106769655060276 2316 60-61 -0.9001173173823958 2316 62-63 -1.2726285851537753 2316 64-65 -0.9324721074986826 2316 66-67 -0.8819765886197644 2316 68-69 -0.6859536286551915 2316 70-71 -0.6217434097758598 2316 72-73 -0.5671832311051617 2316 74-75 -0.7334077753505426 2316 76-77 -0.562214013760034 2316 78-79 -0.5759635203013502 2316 80-81 -0.5806268060077002 2316 82-83 -0.6071647087224719 2316 84-85 -0.4378905428773905 2316 86-87 -0.4213118900629027 2316 88-89 -0.562632514943445 2316 90-91 -0.40899625733126754 2316 92-93 -0.4124010899498032 2316 94-95 -0.5525714072291592 2316 96-97 -0.594060531278636 2316 98-99 -0.4897242856934838 2316 100-101 -0.33523234303698146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 659.0 3 28.0 4 15.0 5 12.0 6 60.0 7 601.0 8 819.0 9 520.0 10 418.0 11 440.0 12 451.0 13 492.0 14 434.0 15 474.0 16 649.0 17 957.0 18 1227.0 19 1564.0 20 2093.0 21 2896.0 22 2838.0 23 2387.0 24 2005.0 25 1737.0 26 1739.0 27 1859.0 28 2067.0 29 2188.0 30 2424.0 31 2984.0 32 3824.0 33 5075.0 34 7949.0 35 13098.0 36 22560.0 37 74923.0 38 188912.0 39 64683.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.81284232942283 25.361634164356495 13.817158917591534 22.00836458862915 2 12.898623014978488 23.687403776516913 43.637379555777244 19.77659365272735 3 13.725734733327895 35.621675292718145 31.579502774886436 19.073087199067523 4 9.70726629989016 20.505508842447586 45.568943271154005 24.218281586508255 5 9.675268770788819 41.67739831315304 38.32552740946447 10.321805506593668 6 22.82965214972811 45.329628173282565 21.15969271787396 10.681026959115366 7 23.090202848511005 34.25142665323934 29.610847360092073 13.04752313815758 8 23.339167965471766 37.398393478000244 23.69476082004556 15.567677736482436 9 26.290802894505905 16.329549759510485 25.440577750284127 31.939069595699483 10-11 21.421652544607962 29.882539956554886 31.85447326399379 16.841334234843362 12-13 22.869385279018633 28.378783410182447 30.607167948284502 18.144663362514414 14-15 22.189611597872634 28.820598404956815 27.514494942955388 21.475295054215163 16-17 18.915175110331433 31.40937844130416 29.81578874841078 19.859657699953626 18-19 19.13460351771017 30.452193146947497 31.479300292243856 18.93390304309848 20-21 19.043819259482913 30.515448107132116 31.851244798612083 18.58948783477289 22-23 20.02789841525497 30.252164284276223 30.42557067119177 19.294366629277036 24-25 19.703139980824545 31.351749760306806 30.550814956855227 18.394295302013422 26-27 18.85642581832825 30.9772239160866 31.29698076664941 18.869369498935743 28-29 19.265880221048505 28.777653534870513 32.828531604639096 19.12793463944189 30-31 19.70363942957062 30.97092603090405 30.87492764166354 18.45050689786179 32-33 19.39561209974222 30.277398610550183 31.659298372184157 18.667690917523437 34-35 19.275855334027515 30.899626987731217 31.579748297599803 18.244769380641465 36-37 19.9216283853315 29.70945478897047 30.91641599010951 19.452500835588523 38-39 19.341329562883438 31.725340299079758 30.690184049079754 18.243146088957054 40-41 18.792719029938816 30.4418877423266 32.531937810078006 18.23345541765658 42-43 19.152828258385103 30.511503988001255 31.201048452598013 19.13461930101563 44-45 18.90451914600023 30.559455134347807 31.31492199777684 19.221103721875117 46-47 19.84003009168861 30.258318938357558 30.702866137506557 19.198784832447274 48-49 18.810129342100694 31.02197869335368 30.42348546833053 19.744406496215095 50-51 19.112964475931946 30.0308592430381 30.000431262713267 20.855745018316686 52-53 19.166483962136432 30.084547270154705 30.67698917310925 20.07197959459961 54-55 19.46517964337322 30.615937253623898 30.330530387084558 19.588352715918322 56-57 18.90386732917409 31.146994932944487 30.253520519238396 19.695617218643026 58-59 18.621491267487492 29.884425271861236 30.854797639375693 20.63928582127558 60-61 19.278062881564345 31.61935488510224 29.766633029480076 19.335949203853335 62-63 18.77052048333677 30.05744537057896 31.902431542469312 19.26960260361496 64-65 18.68443534868562 29.857161172665915 31.143561349042688 20.314842129605776 66-67 18.761373642500732 30.42739138508353 29.84803196300534 20.963203009410396 68-69 18.71642956737565 30.367196446625112 31.010215473663532 19.90615851233571 70-71 19.086126815149196 29.152604913556907 31.550680415137695 20.210587856156202 72-73 18.99207625199771 30.100777998480904 30.10125720912513 20.805888540396257 74-75 19.005887203482462 30.700112182867663 29.924779948990356 20.369220664659522 76-77 18.683507530569905 30.379231039346884 31.34651407779731 19.5907473522859 78-79 19.056763219878814 30.38675225200928 30.36841253573548 20.188071992376432 80-81 18.655598357492323 31.207526002775865 29.639448949701197 20.49742669003061 82-83 19.738628832087482 30.45439128251409 29.960245407008166 19.846734478390257 84-85 18.30739658655955 30.332092619711286 30.390812807232408 20.969697986496755 86-87 18.50832072254713 30.50671996146671 31.454116508088276 19.530842807897887 88-89 19.672207745529697 29.89546829604337 30.907838380207863 19.524485578219068 90-91 19.121101587814973 30.19903089980175 31.47311615426542 19.20675135811786 92-93 19.88167069762539 30.391152734403544 30.32838238165572 19.398794186315342 94-95 19.118256590374926 29.130020196986507 30.67007036601348 21.08165284662509 96-97 20.395377675538704 29.23602250058408 30.32570733665211 20.04289248722511 98-99 19.695054671878875 29.04096886691977 30.77858458117639 20.485391880024967 100-101 19.622203795217395 29.1808264351894 30.752380998030016 20.44458877156319 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 711.0 1 390.5 2 100.0 3 145.0 4 121.5 5 100.0 6 164.5 7 289.5 8 425.0 9 476.5 10 392.0 11 289.0 12 289.0 13 308.5 14 295.5 15 284.0 16 286.0 17 290.5 18 263.5 19 295.0 20 366.5 21 394.0 22 470.0 23 590.0 24 665.5 25 787.0 26 1183.0 27 1677.0 28 1929.0 29 2916.0 30 4751.5 31 6348.0 32 9484.0 33 13851.5 34 18624.0 35 22514.5 36 28213.0 37 33271.0 38 33627.0 39 34004.0 40 30808.5 41 25442.0 42 22231.5 43 20722.0 44 18742.5 45 17890.5 46 16194.5 47 11091.5 48 7934.5 49 6928.5 50 6310.5 51 4082.5 52 1730.0 53 933.0 54 709.5 55 630.5 56 443.5 57 333.5 58 388.5 59 437.0 60 358.5 61 270.0 62 249.5 63 232.0 64 197.0 65 171.5 66 149.5 67 128.5 68 124.0 69 123.0 70 107.5 71 97.5 72 88.5 73 69.0 74 58.5 75 75.0 76 72.5 77 53.0 78 57.5 79 66.5 80 54.0 81 22.0 82 7.5 83 7.5 84 6.5 85 3.5 86 1.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25641761627295084 2 0.25593922519781476 3 0.266463828850809 4 0.26359348239999236 5 0.2576135939607911 6 0.23728197326750672 7 0.24110910186859552 8 0.24350105724427604 9 0.23967392864318723 10-11 0.23680358219237063 12-13 0.243261861706708 14-15 0.24541462154482047 16-17 0.191954418898361 18-19 0.18645292153429585 20-21 0.17975544648239042 22-23 0.20092425155716293 24-25 0.20762172660906839 26-27 0.2095352909096128 28-29 0.2091764976032607 30-31 0.20893730206569266 32-33 0.2039141957767636 34-35 0.18884487690997637 36-37 0.16683888745371567 38-39 0.1884860836036243 40-41 0.1754499268061655 42-43 0.16528411645952332 44-45 0.15404192619382492 46-47 0.16193537893357063 48-49 0.1642077365404671 50-51 0.16480572538438723 52-53 0.1740147535807572 54-55 0.18394136838983133 56-57 0.1828649884707751 58-59 0.19267200551106517 60-61 0.20833931322177252 62-63 0.191834821129577 64-65 0.18645292153429585 66-67 0.1699484294421003 68-69 0.15834744587004984 70-71 0.17042682051723643 72-73 0.17102480936115655 74-75 0.21336241951070162 76-77 0.2077413243778524 78-79 0.2249634030827521 80-81 0.2155151793488141 82-83 0.21132925744137315 84-85 0.19972827386932268 86-87 0.1833433795459112 88-89 0.17473234019346134 90-91 0.1603806079393783 92-93 0.16109819455208244 94-95 0.16241377000870671 96-97 0.17832027325698213 98-99 0.1709052115923725 100-101 0.1927916032798492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 418068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.400690796712498 #Duplication Level Percentage of deduplicated Percentage of total 1 71.84420962444666 9.627620387114058 2 7.884121090961018 2.1130533788761636 3 3.2896615736113093 1.3225121272137546 4 1.9848636298729117 1.0639417511026914 5 1.4243895473368555 0.954390194896524 6 1.0995287733828358 0.8840667068515169 7 0.9138940454091103 0.8572768066438953 8 0.7318292160502642 0.7845613632232077 9 0.6747108382121948 0.81374321880651 >10 7.032700271312295 21.090348938450205 >50 1.627873768384978 15.703187041342556 >100 1.4029701556475795 35.6604667183329 >500 0.07675282021990576 7.1019547059330055 >1k 0.01249464515207768 2.0228766612130085 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1585 0.37912492704536105 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1551 0.3709922787680473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.28464268970598083 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1088 0.26024474487403965 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1036 0.24780657692050098 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1006 0.24063071079345943 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1001 0.2394347331056192 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 945 0.22603978300180833 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 939 0.22460460977640004 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 933 0.22316943655099172 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 931 0.22269104547585558 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 911 0.21790713472449458 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 893 0.2136016150482697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.20475138015825178 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 851 0.2035554024704115 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 815 0.19494436311796168 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 793 0.18968206129146456 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 779 0.18633332376551182 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 761 0.1820278040892869 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 761 0.1820278040892869 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 747 0.1786790665633342 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 741 0.1772438933379259 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 738 0.17652630672522174 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 734 0.1755695245749495 No Hit TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC 727 0.17389515581197318 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 720 0.17222078704899682 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 707 0.16911124506061215 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 684 0.16360974769654696 No Hit GTGTTATGGCTGGGGGTTTCATGTTGATAATAGTGGTAATAAAATTAATT 660 0.15786905479491375 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 652 0.15595549049436935 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 647 0.15475951280652908 No Hit CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG 639 0.15284594850598468 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 633 0.15141077528057637 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 622 0.1487796243673278 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 620 0.1483012332921917 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 606 0.144952495766239 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 591 0.14136456270271822 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 585 0.13992938947730993 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 573 0.1370590430264933 No Hit GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC 571 0.13658065195135718 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 569 0.1361022608762211 No Hit ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT 561 0.1341886965756767 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 553 0.13227513227513227 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 537 0.12844800367404346 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 535 0.12796961259890738 No Hit ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA 532 0.1272520259862032 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 512 0.12246811523484219 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 511 0.12222891969727412 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 510 0.12198972415970606 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 506 0.12103294200943386 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 497 0.11888018217132142 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 486 0.11624903125807284 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 480 0.11481385803266454 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 479 0.11457466249509649 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 479 0.11457466249509649 No Hit GTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTC 478 0.11433546695752844 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 477 0.11409627141996039 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 476 0.11385707588239234 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 475 0.11361788034482428 No Hit ATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCT 466 0.11146512050671184 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 463 0.11074753389400767 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 463 0.11074753389400767 No Hit CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA 462 0.11050833835643961 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 458 0.10955155620616741 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 449 0.10739879636805497 No Hit GTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTACTGC 443 0.10596362314264664 No Hit ATCAATGGGAGCAGTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCC 442 0.1057244276050786 No Hit GCCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTAC 430 0.10285408115426199 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 426 0.10189729900398979 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 423 0.10117971239128563 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 423 0.10117971239128563 No Hit GTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACG 422 0.10094051685371759 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 420 0.10046212577858146 No Hit CCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACA 420 0.10046212577858146 No Hit GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT 419 0.10022293024101342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3919553756805112E-4 0.0 3 0.0 0.0 0.0 2.3919553756805112E-4 0.0 4 0.0 0.0 0.0 7.175866127041533E-4 0.0 5 0.0 0.0 0.0 7.175866127041533E-4 0.0 6 0.0 0.0 0.0 7.175866127041533E-4 0.0 7 0.0 0.0 0.0 7.175866127041533E-4 0.0 8 0.0 0.0 0.0 7.175866127041533E-4 0.0 9 0.0 0.0 0.0 0.0011959776878402557 0.0 10-11 0.0 0.0 0.0 0.0013155754566242812 0.0 12-13 0.0 0.0 0.0 0.001674368762976358 0.0 14-15 0.0 0.0 0.0 0.001913564300544409 0.0 16-17 0.0 0.0 0.0 0.002750748682032588 0.0 18-19 0.0 0.0 0.0 0.003348737525952716 0.0 20-21 0.0 0.0 0.0 0.0045447152137929716 0.0 22-23 0.0 0.0 0.0 0.006577877283121406 0.0 24-25 0.0 0.0 0.0 0.009328625965153993 0.0 26-27 0.0 0.0 0.0 0.012079374647186582 0.0 28-29 0.0 0.0 0.0 0.017461274242467732 0.0 30-31 0.0 0.0 0.0 0.023919553756805115 0.0 32-33 0.0 0.0 0.0 0.03372657079709521 0.0 34-35 0.0 0.0 0.0 0.05082905173321087 0.0 36-37 0.0 0.0 0.0 0.07235665011433547 0.0 38-39 0.0 0.0 0.0 0.11170431604427988 0.0 40-41 0.0 0.0 0.0 0.1622941722399227 0.0 42-43 0.0 0.0 0.0 0.20546896677095594 0.0 44-45 0.0 0.0 0.0 0.24983973898982942 0.0 46-47 0.0 0.0 0.0 0.30748586354372975 0.0 48-49 0.0 0.0 0.0 0.36644756355425434 0.0 50-51 0.0 0.0 0.0 0.43533587837385307 0.0 52-53 0.0 0.0 0.0 0.5043437909622358 0.0 54-55 0.0 0.0 0.0 0.5833979161284767 0.0 56-57 0.0 0.0 0.0 0.6637676167513419 0.0 58-59 0.0 0.0 0.0 0.7533463455705771 0.0 60-61 0.0 0.0 0.0 0.8506989293607738 0.0 62-63 0.0 0.0 0.0 0.9557057703531482 0.0 64-65 0.0 0.0 0.0 1.0681276730101323 0.0 66-67 0.0 0.0 0.0 1.1995656209037764 0.0 68-69 0.0 0.0 0.0 1.3488236363462405 0.0 70-71 0.0 0.0 0.0 1.5264263229905182 0.0 72-73 0.0 0.0 0.0 1.708214931542237 0.0 74-75 0.0 0.0 0.0 1.8972990039897817 0.0 76-77 0.0 0.0 0.0 2.0945157247146398 0.0 78-79 0.0 0.0 0.0 2.331199709138226 0.0 80-81 0.0 0.0 0.0 2.591922845087402 0.0 82-83 0.0 0.0 0.0 2.882904216538936 0.0 84-85 0.0 0.0 0.0 3.1841709961058964 0.0 86-87 0.0 0.0 0.0 3.4774247251643273 0.0 88-89 0.0 0.0 0.0 3.815766813054336 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGTCG 20 0.0020707094 71.27114 9 GACATAT 135 0.0 70.39124 1 CATTCCG 35 3.407191E-6 67.877266 9 ACATATA 140 0.0 64.483406 2 CCTTTAG 45 2.0402877E-7 63.35212 1 GAGTATG 40 7.523833E-6 59.392616 1 AGGGTCT 40 7.523833E-6 59.392616 5 AGTATGG 40 7.523833E-6 59.392616 2 GGCATTC 45 1.5116326E-5 52.793434 7 CTTTAGG 45 1.5116326E-5 52.793434 2 GTCTTCT 45 1.5116326E-5 52.793434 8 CATATAG 185 0.0 48.798256 3 GAGAGCC 40 5.3763384E-4 47.514095 8 CCTCATC 65 2.5801164E-6 43.85916 3 TGGGGAG 70 4.294814E-6 40.72636 6 ATGGGCG 50 0.001612284 38.011272 5 TGGGCGT 50 0.001612284 38.011272 6 GGATATG 365 0.0 37.750923 1 GGATGAC 360 0.0 36.955402 8 TATGGGC 65 1.3244202E-4 36.5493 2 >>END_MODULE