FastQCFastQC Report
Fri 10 Feb 2017
SRR2568612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418068
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20870.4992010869045227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.41763540859381726No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC15120.3616636528028933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.33917927227149647No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11170.26718141546351315No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT10600.25354726982213416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.24350105724427604No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG10090.2413482974061636No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC10070.24086990633102748No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10010.2394347331056192No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9930.2375211688050748No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA9400.22484380531396808No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC9300.22245184993828754No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT8980.21479759273610993No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG8670.20738253107150031No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG8660.20714333553393227No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT8610.20594735784609203No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT8360.19996746940689072No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7930.18968206129146456No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT7900.1889644746787604No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG7650.18298458623955913No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT7650.18298458623955913No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7550.1805926308638786No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7520.17987504425117445No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7460.17843987102576614No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT7420.17748308887549394TruSeq Adapter, Index 1 (95% over 21bp)
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC7380.17652630672522174No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT7360.17604791565008562No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7330.17533032903738147No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC7090.16958963613574823No Hit
GTGTTATGGCTGGGGGTTTCATGTTGATAATAGTGGTAATAAAATTAATT7020.1679152673727719No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG6720.16073940124573036No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG6400.15308514404355272No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6380.1526067529684166No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG6260.1497364065176No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG6160.1473444511419195No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC6080.1454308868413751No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA6040.1444741046911029No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT5940.14208214931542237No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT5620.13442789211324474No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5520.13203593673756422No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC5500.1315575456624281No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT5490.13131835012486007RNA PCR Primer, Index 1 (95% over 23bp)
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT5450.13036156797458787No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT5420.1296439813618837No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG5410.12940478582431567No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5250.12557765722322684No Hit
ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA5230.12509926614809075No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG5140.12294650630997829No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5120.12246811523484219No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT5120.12246811523484219No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC5110.12222891969727412No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT5050.12079374647186582No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC5030.12031535539672972No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5030.12031535539672972No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG4990.11935857324645752No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT4980.11911937770888946No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC4890.11696661787077701No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA4800.11481385803266454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4800.11481385803266454No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT4750.11361788034482428No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT4680.11194351158184794No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA4670.11170431604427988No Hit
ATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCT4570.10931236066859937No Hit
GTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTC4540.10859477405589521No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA4410.10548523206751054No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG4360.1042892543796703No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA4350.10405005884210224No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC4340.10381086330453419No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG4340.10381086330453419No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT4320.10333247222939809No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG4290.10261488561669393No Hit
CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA4240.10141890792885369No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC4240.10141890792885369No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC4210.10070132131614953No Hit
GTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTACTGC4210.10070132131614953No Hit
GTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACG4200.10046212577858146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG301.3601111E-679.222432
GACATAT1700.078.29041
ACATATA1900.070.04932
GAGAGCC603.2923708E-1063.324888
CATATAG2200.060.4898833
CATATGC250.005007448657.033323
GCAGATC654.4148692E-851.147028
TGGGTAT957.2759576E-1249.993336
CCCGCCG205.3829345E-447.50786674-75
TCGCCCG205.3845224E-447.50502472-73
GTCTCGC205.3845224E-447.50502468-69
CGCGCCG205.3845224E-447.50502480-81
TCTCGCC205.3845224E-447.50502470-71
CCGCGCC205.3845224E-447.50502478-79
GGCCCGT205.389288E-447.49650264-65
GCCCGTC205.389288E-447.49650264-65
AGTCCGC205.3908775E-447.4936642-43
CGGCCCG205.3908775E-447.4936662-63
GCGCACC205.3908775E-447.4936654-55
GCACCAC205.3908775E-447.4936656-57
Produced by FastQC (version 0.11.5)