##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568612_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418068 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.12640048987246 34.0 33.0 34.0 31.0 34.0 2 33.19529119664744 34.0 33.0 34.0 31.0 34.0 3 33.25593683324244 34.0 34.0 34.0 31.0 34.0 4 36.55286221380254 37.0 37.0 37.0 35.0 37.0 5 36.536867208205365 37.0 37.0 37.0 35.0 37.0 6 36.605088167475145 37.0 37.0 37.0 35.0 37.0 7 36.57150272204522 37.0 37.0 37.0 35.0 37.0 8 36.590403953423845 37.0 37.0 37.0 35.0 37.0 9 38.44441095706919 39.0 39.0 39.0 37.0 39.0 10-11 38.44350081804874 39.0 39.0 39.0 37.0 39.0 12-13 38.45608848321326 39.0 39.0 39.0 37.0 39.0 14-15 40.02498756183205 41.0 40.0 41.0 38.0 41.0 16-17 40.00756097094252 41.0 40.0 41.0 38.0 41.0 18-19 40.093621133404135 41.0 40.0 41.0 38.0 41.0 20-21 40.094848206511855 41.0 40.0 41.0 38.0 41.0 22-23 40.03774266387286 41.0 40.0 41.0 38.0 41.0 24-25 40.000395868614675 41.0 40.0 41.0 38.0 41.0 26-27 39.91024426648296 41.0 40.0 41.0 38.0 41.0 28-29 39.84998493068113 41.0 40.0 41.0 38.0 41.0 30-31 39.73824353932853 41.0 40.0 41.0 37.0 41.0 32-33 39.61294932881732 41.0 40.0 41.0 36.5 41.0 34-35 39.51808916252858 41.0 40.0 41.0 36.0 41.0 36-37 39.42074973449296 41.0 40.0 41.0 36.0 41.0 38-39 39.3557029000067 41.0 40.0 41.0 36.0 41.0 40-41 39.25398978156663 41.0 40.0 41.0 35.0 41.0 42-43 39.128836696422596 41.0 39.0 41.0 35.0 41.0 44-45 38.96891893184841 41.0 39.0 41.0 35.0 41.0 46-47 38.796997139221375 41.0 39.0 41.0 35.0 41.0 48-49 38.62580608896161 40.0 39.0 41.0 35.0 41.0 50-51 38.482202656027255 40.0 39.0 41.0 35.0 41.0 52-53 38.41895337600582 40.0 39.0 41.0 35.0 41.0 54-55 38.23958542629428 40.0 38.5 41.0 35.0 41.0 56-57 38.07080905498627 40.0 38.0 41.0 34.5 41.0 58-59 38.02125132753524 40.0 38.0 41.0 34.5 41.0 60-61 38.01875412612302 40.0 38.0 41.0 35.0 41.0 62-63 37.79233282623879 40.0 38.0 41.0 34.0 41.0 64-65 37.49654601643752 40.0 37.0 41.0 34.0 41.0 66-67 37.372085402374736 39.0 37.0 41.0 34.0 41.0 68-69 36.97548484935466 39.0 36.5 41.0 33.5 41.0 70-71 36.543746471865816 39.0 36.0 41.0 33.0 41.0 72-73 36.15806639111341 37.5 35.5 40.0 33.0 41.0 74-75 35.68324291742013 37.0 35.0 39.0 32.0 41.0 76-77 33.72070452653635 35.5 33.0 38.0 29.0 39.0 78-79 34.704227541930976 36.0 35.0 38.0 31.0 39.0 80-81 34.51662887377173 36.0 35.0 37.0 31.0 39.0 82-83 34.19010424141527 35.5 35.0 37.0 31.5 39.0 84-85 33.82768712266904 35.0 35.0 36.5 31.0 38.0 86-87 33.64482572213133 35.0 35.0 36.0 31.0 37.0 88-89 33.44227613689639 35.0 35.0 36.0 31.0 37.0 90-91 33.25791258838275 35.0 35.0 36.0 31.0 36.5 92-93 33.1479950630041 35.0 35.0 35.0 31.0 36.0 94-95 33.04875283446712 35.0 35.0 35.0 30.0 36.0 96-97 32.972209066467656 35.0 35.0 35.0 30.0 36.0 98-99 32.91158017356028 35.0 35.0 35.0 30.0 36.0 100-101 32.20689457217486 34.5 33.5 35.0 27.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1933047184963428 1101 2 0.12992563372202426 1101 3 0.2085963159004791 1101 4 0.15313994754937 1101 5 0.10633943797294876 1101 6 0.07261624398212518 1101 7 0.11888775998959034 1101 8 0.15968338610655763 1101 9 0.2490645526102071 1101 10-11 0.11393603096315985 1101 12-13 0.0995652938882543 1101 14-15 0.16119741332384052 1101 16-17 0.1903245906795945 1101 18-19 0.16162138875314724 1101 20-21 0.15781939863154548 1101 22-23 0.20844604318735804 1101 24-25 0.23819371945769063 1101 26-27 0.2226700178008585 1101 28-29 0.1535348326355006 1101 30-31 0.26391483231105894 1101 32-33 0.2429037723273737 1101 34-35 0.19743204909694612 1101 36-37 0.29693892099739827 1101 38-39 0.2614252277338025 1101 40-41 0.3315767132622014 1101 42-43 0.2213254419509596 1101 44-45 0.09372147056954105 1101 46-47 0.10581876799187029 1101 48-49 0.25071101732446266 1101 50-51 0.26297558324937853 1101 52-53 0.2601531725445696 1101 54-55 0.06194657079189625 1101 56-57 0.20759916811532264 1101 58-59 0.2005849159871289 1101 60-61 0.3972668984813197 1101 62-63 0.24082545122983134 1101 64-65 0.2687482460327004 1101 66-67 0.08709287733542936 1101 68-69 0.11282781126522679 1101 70-71 0.11291575801018183 1101 72-73 0.028675557781888017 1101 74-75 -0.2631352062926098 1101 76-77 -0.16568624068957405 1101 78-79 0.0689867641403481 1101 80-81 0.1563739295390718 1101 82-83 -0.11362827714309276 1101 84-85 -0.09406568533212578 1101 86-87 -0.01195531173397768 1101 88-89 0.026030874081989452 1101 90-91 0.06192717583725482 1101 92-93 0.05021386097646996 1101 94-95 -0.10059442885938097 1101 96-97 0.04963890691771411 1101 98-99 -0.019807034058068496 1101 100-101 -0.03152377685110963 1102 1 0.20725694889466695 1102 2 0.15505084006768044 1102 3 0.12120834876655806 1102 4 0.1980277048402499 1102 5 0.13778791451250072 1102 6 0.14592141171665673 1102 7 0.057856078354120655 1102 8 0.1351457402656493 1102 9 0.11310491708425019 1102 10-11 0.20288149975250747 1102 12-13 0.14257845337828456 1102 14-15 0.11763399875793823 1102 16-17 0.13543431527226346 1102 18-19 0.10684769281105133 1102 20-21 0.08701835781008072 1102 22-23 0.22336821473637514 1102 24-25 0.24480144354674138 1102 26-27 0.15183633472913272 1102 28-29 0.2355601443689892 1102 30-31 0.1321520575332542 1102 32-33 0.12225801535358727 1102 34-35 0.27967653022506056 1102 36-37 0.41267901407444185 1102 38-39 0.5185668857735877 1102 40-41 0.5001972517660747 1102 42-43 0.5226227382399955 1102 44-45 0.42878017998823026 1102 46-47 0.5612807490966745 1102 48-49 0.6031120879902758 1102 50-51 0.517160786051015 1102 52-53 0.4807261839320418 1102 54-55 0.6064286315437926 1102 56-57 0.6367375059719436 1102 58-59 0.5701744630059622 1102 60-61 0.858562516026069 1102 62-63 0.7295079167456322 1102 64-65 0.5809713698027963 1102 66-67 0.6298822042522261 1102 68-69 0.7824742490630925 1102 70-71 0.719856433051909 1102 72-73 0.5402075984821053 1102 74-75 0.5609184016086814 1102 76-77 0.3580027066444842 1102 78-79 0.42554739012544474 1102 80-81 0.4374656729150317 1102 82-83 0.34198292567719335 1102 84-85 0.47310740253148253 1102 86-87 0.4034033333328395 1102 88-89 0.33589909265450046 1102 90-91 0.3025518552437205 1102 92-93 0.38734768487619675 1102 94-95 0.30971399377381914 1102 96-97 0.32807730166510396 1102 98-99 0.39399877253193694 1102 100-101 0.39106279505976005 1103 1 0.13097701862818667 1103 2 0.08596662617078721 1103 3 0.11219813778708954 1103 4 0.12281512434429231 1103 5 0.05725637432678354 1103 6 0.1606241548055678 1103 7 0.11972678671858716 1103 8 0.13607077673343326 1103 9 0.2066937027959952 1103 10-11 0.15178212948886483 1103 12-13 0.1945850709468644 1103 14-15 0.3827903654993179 1103 16-17 0.32343018818648517 1103 18-19 0.28401441284520246 1103 20-21 0.33414985530180985 1103 22-23 0.2616193621373739 1103 24-25 0.2458198731194443 1103 26-27 0.316506167866784 1103 28-29 0.2735755853623374 1103 30-31 0.31582961116757957 1103 32-33 0.382526809604137 1103 34-35 0.42140723787796475 1103 36-37 0.23557010881952323 1103 38-39 0.29082112848892194 1103 40-41 0.32919447665666723 1103 42-43 0.2685356780770505 1103 44-45 0.33332353217807054 1103 46-47 0.3246848832981115 1103 48-49 0.47356250959342816 1103 50-51 0.5859559260517315 1103 52-53 0.388449348979897 1103 54-55 0.14235829319390803 1103 56-57 0.29135201481314965 1103 58-59 0.5973696457006596 1103 60-61 0.577298062331252 1103 62-63 0.45148109068806974 1103 64-65 0.40395141036208315 1103 66-67 0.42397709164682595 1103 68-69 0.3387057928066426 1103 70-71 0.3718603822460622 1103 72-73 0.2754692770675078 1103 74-75 0.41983255110880435 1103 76-77 0.1297410636374039 1103 78-79 0.635539562893797 1103 80-81 0.4297318343694698 1103 82-83 0.5643652503649932 1103 84-85 0.49260877229703226 1103 86-87 0.5062335891409973 1103 88-89 0.488739132502225 1103 90-91 0.4402533175129122 1103 92-93 0.410860510862598 1103 94-95 0.550490312840239 1103 96-97 0.35416066168218663 1103 98-99 0.4487893940852885 1103 100-101 0.4737201402050957 1104 1 -0.07698575671047081 1104 2 -0.022020540327019944 1104 3 -0.0699569149353394 1104 4 -0.01839092466317993 1104 5 -0.006742274778254398 1104 6 -2.7306460228970764E-4 1104 7 0.023738194276091917 1104 8 0.05167736609888607 1104 9 0.24813991090886844 1104 10-11 0.16363060410330377 1104 12-13 0.035376470248898784 1104 14-15 0.14517356937188453 1104 16-17 0.12953923543027912 1104 18-19 0.05130868279904632 1104 20-21 -0.02705975856738263 1104 22-23 0.008762782153134197 1104 24-25 0.11396220491425879 1104 26-27 0.16959164949836492 1104 28-29 0.05989026884014237 1104 30-31 0.22468641630098318 1104 32-33 0.12482014143647291 1104 34-35 -0.12853366046719117 1104 36-37 0.08515170662807492 1104 38-39 0.056863006232923397 1104 40-41 -0.07378030663726776 1104 42-43 -0.1315778346251122 1104 44-45 0.15149682198256897 1104 46-47 -0.07487982832333273 1104 48-49 0.15933871132707367 1104 50-51 0.20074916407191523 1104 52-53 0.35364035051841824 1104 54-55 0.3661143440776229 1104 56-57 0.316559339665865 1104 58-59 -0.07881426363928057 1104 60-61 0.1670950528374675 1104 62-63 0.27781603759039086 1104 64-65 0.25985594711768556 1104 66-67 0.05933395306751521 1104 68-69 0.13637174650096284 1104 70-71 -0.07696039902152307 1104 72-73 0.19113215372329506 1104 74-75 -0.03301746202489397 1104 76-77 -0.11414238414675282 1104 78-79 -0.009278253909833722 1104 80-81 0.08724712575001092 1104 82-83 -0.14282826533915483 1104 84-85 -0.11652759304501359 1104 86-87 0.01952677087390242 1104 88-89 -0.12982535397006245 1104 90-91 -0.10493130479937207 1104 92-93 0.10938109324843737 1104 94-95 -0.1007911611362644 1104 96-97 0.05182755349801482 1104 98-99 -0.10764799568543282 1104 100-101 -0.1330474683355547 1105 1 0.18124390797553502 1105 2 0.22603441279029823 1105 3 0.16936649093245393 1105 4 0.12906983174842424 1105 5 0.15436807488675441 1105 6 0.04202213103113195 1105 7 0.05884981779563958 1105 8 0.06733448960633837 1105 9 0.05374809582739459 1105 10-11 0.1733215734421094 1105 12-13 0.16454773918540155 1105 14-15 0.29906406625578086 1105 16-17 0.26779276473296676 1105 18-19 0.2605715402644009 1105 20-21 0.21934240859780374 1105 22-23 0.24593756628316754 1105 24-25 0.2441211142367834 1105 26-27 0.23918603163860297 1105 28-29 0.30936823035038685 1105 30-31 0.1655569910673762 1105 32-33 0.2896190256043951 1105 34-35 0.38489824878276835 1105 36-37 0.2727872552320676 1105 38-39 0.23430771852724064 1105 40-41 0.32220705156579044 1105 42-43 0.36228670142577357 1105 44-45 0.44392706232183343 1105 46-47 0.36674860926988373 1105 48-49 0.14511668976830094 1105 50-51 0.33722897186500944 1105 52-53 0.49669099026751695 1105 54-55 0.6031722238354291 1105 56-57 0.42028576731012635 1105 58-59 0.45221376922294354 1105 60-61 0.3753299580642988 1105 62-63 0.44925428386356003 1105 64-65 0.5272444646896162 1105 66-67 0.5052596341362587 1105 68-69 0.7129239543811252 1105 70-71 0.7890550968545753 1105 72-73 0.6412059244133488 1105 74-75 0.5684555330651193 1105 76-77 0.37053911190671585 1105 78-79 0.7264211164190328 1105 80-81 0.7297817737872307 1105 82-83 0.7350106968725143 1105 84-85 0.644592431464595 1105 86-87 0.7305270691816119 1105 88-89 0.6423683364187838 1105 90-91 0.5676356733435384 1105 92-93 0.6302787575796955 1105 94-95 0.5035963808888368 1105 96-97 0.5574591192909892 1105 98-99 0.7142542400099003 1105 100-101 0.5136634876197164 1106 1 0.1544890879114007 1106 2 0.1595155482271693 1106 3 0.1532613402930778 1106 4 0.08817351756337644 1106 5 0.0431601885888071 1106 6 0.07507965508196435 1106 7 0.06337889653985229 1106 8 0.07253659578631755 1106 9 0.029376584682857754 1106 10-11 0.13887444568565854 1106 12-13 0.042539742035863526 1106 14-15 0.1311872698663734 1106 16-17 0.24600251175383647 1106 18-19 0.2451117041663693 1106 20-21 0.28745278509727257 1106 22-23 0.29380565290575333 1106 24-25 0.10988381268089853 1106 26-27 0.10027712788152598 1106 28-29 0.050395191037665654 1106 30-31 0.10666709043789524 1106 32-33 5.4909843676398395E-5 1106 34-35 0.16066926109520807 1106 36-37 -0.047035382243684865 1106 38-39 -0.15884719655253576 1106 40-41 -0.06714327900944994 1106 42-43 -0.009679726382138654 1106 44-45 0.258678240119842 1106 46-47 -0.0711507533335407 1106 48-49 0.07524852396601744 1106 50-51 0.14979874897415613 1106 52-53 0.27861426513984355 1106 54-55 0.34461091895400386 1106 56-57 0.1693969751964275 1106 58-59 -0.0283674097627582 1106 60-61 -0.02600026086756202 1106 62-63 -0.08612716433312784 1106 64-65 0.3179923169403054 1106 66-67 0.45433917014323555 1106 68-69 0.49079718768410174 1106 70-71 0.2648261091670179 1106 72-73 0.20966865057037154 1106 74-75 0.24170467198589307 1106 76-77 0.08370273675330253 1106 78-79 0.2602585604983645 1106 80-81 0.13602905198509774 1106 82-83 0.09521102150927874 1106 84-85 0.06516727607443329 1106 86-87 0.1480891262701718 1106 88-89 0.16480786292418514 1106 90-91 0.2462096469767019 1106 92-93 0.14515662289503695 1106 94-95 0.05139320578293649 1106 96-97 0.15616453122336793 1106 98-99 0.05759720687319714 1106 100-101 -0.09487208385976231 1107 1 0.1429447686911729 1107 2 0.18092127469686403 1107 3 0.205987505385977 1107 4 0.10492648740803645 1107 5 0.12216567137893719 1107 6 0.12868915858485508 1107 7 0.07714679175764161 1107 8 0.04624966310279177 1107 9 0.09762970682723449 1107 10-11 0.0837076774858474 1107 12-13 0.08371016542910326 1107 14-15 0.17081946602841924 1107 16-17 0.16438508866519186 1107 18-19 0.1500386002979539 1107 20-21 0.22752665046431986 1107 22-23 0.07926864407237133 1107 24-25 0.09458530521821018 1107 26-27 0.2676431041227687 1107 28-29 0.2750715541202169 1107 30-31 0.2734543277208701 1107 32-33 0.23104028906999474 1107 34-35 0.3319798002362333 1107 36-37 0.36967189768321873 1107 38-39 0.07214199016692646 1107 40-41 0.21217822571184541 1107 42-43 -0.05306611078664503 1107 44-45 -0.020669917053474762 1107 46-47 0.012153389219001554 1107 48-49 -0.004659334699844919 1107 50-51 0.05159588573931018 1107 52-53 0.06561929103079223 1107 54-55 0.027751339301246958 1107 56-57 0.004330724815673648 1107 58-59 -0.0126234256019373 1107 60-61 0.07854207845622341 1107 62-63 0.309269576452742 1107 64-65 0.10231686765611414 1107 66-67 0.21540755272014422 1107 68-69 0.3065030969134668 1107 70-71 0.201697872797844 1107 72-73 0.09981105081404706 1107 74-75 0.1776588297684114 1107 76-77 0.10950611780722141 1107 78-79 0.22310884986784174 1107 80-81 0.3649012725150982 1107 82-83 0.3241441619296239 1107 84-85 0.4213849123826918 1107 86-87 0.273271885941206 1107 88-89 0.2053081675236399 1107 90-91 0.3507786387525158 1107 92-93 0.2939229951155653 1107 94-95 0.17550605987979395 1107 96-97 0.469570714592237 1107 98-99 0.4025560859472179 1107 100-101 0.19484006965836187 1108 1 0.08750259182042441 1108 2 0.035172975377136595 1108 3 0.028776621032235994 1108 4 0.05288567108150488 1108 5 0.11043097750138031 1108 6 0.11406330824471667 1108 7 0.08939168971681966 1108 8 0.03536530936129623 1108 9 0.08147324424220415 1108 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950.0 19 1222.0 20 1757.0 21 2703.0 22 3194.0 23 2378.0 24 1895.0 25 1754.0 26 2081.0 27 2381.0 28 2952.0 29 3295.0 30 3135.0 31 3182.0 32 3733.0 33 4707.0 34 6804.0 35 10588.0 36 21411.0 37 74632.0 38 195990.0 39 66473.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.20463685319822 25.479726028052696 13.975153050605385 22.340484068143702 2 12.757910449903473 23.776921365570793 43.78730356942082 19.67786461510491 3 13.433189314883661 35.54026057291592 32.30463012138698 18.72191999081344 4 9.300161696183395 21.15804127558196 45.30363481538888 24.238162212845758 5 9.317825155017989 41.65773558906836 38.85162290438643 10.172816351527214 6 22.54035228718773 45.773893242247674 20.842542361529702 10.843212109034894 7 22.796052316847977 34.22864223045055 29.44018676387573 13.535118688825742 8 22.990039897815667 37.74338145947549 23.838705665107113 15.42787297760173 9 25.846513007453332 16.087335074676847 25.319565238190915 32.7465866796789 10-11 21.084847441086136 29.84406842905939 32.16462393677584 16.906460193078637 12-13 22.832051245251968 28.2275849861745 30.639273993704375 18.30108977486916 14-15 22.07009386032894 28.66818316637485 27.45605977974875 21.80566319354746 16-17 18.82181846015481 31.44907048614101 29.8372513562387 19.891859697465485 18-19 19.131576681305436 30.0506137757494 31.559100433422316 19.258709109522854 20-21 18.911875580049177 30.23395715529531 31.92471081259508 18.929456452060432 22-23 19.852392433766756 30.226063702555567 30.440622099754105 19.480921763923572 24-25 19.55244122965642 31.327559153056438 30.941377957652822 18.178621659634317 26-27 18.873455387426866 30.882085603708504 31.639980290193414 18.604478718671213 28-29 19.083584192353552 28.32703644302839 33.53162856838734 19.057750796230717 30-31 19.57228934395055 30.84547065872847 31.23010168323913 18.35213831408185 32-33 19.349070955643157 29.933813961498718 32.03672165027364 18.680393432584484 34-35 18.949494804523795 30.719688271427753 32.301414164609525 18.029402759438927 36-37 19.91058838725418 29.381450080310188 31.372042486063823 19.33591904637181 38-39 19.158007788206703 31.342867667460794 31.22745582058421 18.27166872374829 40-41 18.48871559962206 30.030019973448468 33.21129994857136 18.269964478358112 42-43 19.18022513191697 30.001722216109417 31.7278850707783 19.090167581195313 44-45 18.656481684133983 30.325677852686116 31.82890760202025 19.18893286115965 46-47 19.874924354230302 29.72573774060139 30.97159067804921 19.4277472271191 48-49 18.838083756709434 30.498866213151928 30.718686912176967 19.94436311796167 50-51 18.888314819598726 29.773027354401677 30.537735488006735 20.800922337992862 52-53 18.91261578572838 29.749382575780224 31.47242969137759 19.865571947113803 54-55 19.30648544822621 30.337703529884536 30.69435175417824 19.661459267711017 56-57 18.956592666655503 31.059314026833277 30.256564202046103 19.727529104465116 58-59 18.190124800420985 30.040723088974868 31.34792826517249 20.42122384543166 60-61 19.220437823511965 32.06870652621105 29.703182257431806 19.007673392845184 62-63 18.55177156525639 30.72339662131858 31.679234564209896 19.045597249215128 64-65 18.423702704383558 30.599049901689224 31.00939573556075 19.967851658366463 66-67 18.529827557749854 31.05706692692075 29.657364018437853 20.75574149689154 68-69 18.605747092067098 31.004156325688488 30.738031875130822 19.652064707113595 70-71 18.81255382260071 29.64704334513444 31.52497368672854 20.015429145536313 72-73 18.860740870101925 30.54782378827225 29.872598284484646 20.718837057141183 74-75 18.76555732451697 31.006757007300056 29.8826225657011 20.345063102481873 76-77 18.46001289462887 30.777493489887046 31.224260083946664 19.538233531537415 78-79 18.919293881261062 30.67430512366646 30.22066210591781 20.185738889154667 80-81 18.422018963139454 31.488093315774428 29.473603630435274 20.61628409065084 82-83 19.51328036974042 30.822347095733964 29.745372862258773 19.91899967226684 84-85 18.197650565589075 30.52626038986593 30.2860779085943 20.990011135950702 86-87 18.218726150916403 30.763433837408762 31.253035337912355 19.76480467376248 88-89 19.353669694595773 30.080193277160166 30.644237266850453 19.921899761393604 90-91 18.917945884137225 30.298735255209568 31.202859131782724 19.580459728870487 92-93 19.591537940752186 30.650682883300995 29.94459422928324 19.813184946663572 94-95 18.813755980861245 29.273444976076558 30.405980861244018 21.506818181818183 96-97 19.985143469585978 29.431839386791324 30.117249364829497 20.4657677787932 98-99 19.060599355313283 29.08876702170286 30.664003396865073 21.186630226118783 100-101 18.90438266080206 29.346645160981538 30.724811785370708 21.02416039284569 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75.0 1 51.0 2 73.0 3 133.0 4 103.5 5 82.5 6 151.5 7 298.5 8 447.5 9 470.0 10 384.5 11 290.0 12 263.0 13 269.5 14 278.5 15 289.0 16 284.5 17 277.5 18 287.0 19 299.0 20 342.5 21 405.0 22 484.0 23 588.0 24 651.0 25 757.5 26 1114.0 27 1583.5 28 1801.5 29 2747.0 30 4616.0 31 6203.0 32 9404.0 33 13969.0 34 18790.0 35 23036.0 36 28619.5 37 33523.0 38 34142.5 39 34343.0 40 31143.5 41 25872.5 42 22680.5 43 21031.5 44 18901.0 45 18117.0 46 16343.0 47 11172.0 48 8216.0 49 7136.5 50 6306.0 51 4025.5 52 1614.0 53 785.0 54 540.5 55 444.0 56 278.0 57 198.5 58 229.5 59 229.0 60 148.0 61 86.0 62 75.0 63 64.5 64 42.0 65 29.5 66 23.0 67 19.0 68 21.5 69 25.5 70 25.0 71 25.0 72 24.0 73 18.0 74 17.5 75 44.0 76 46.5 77 21.0 78 52.0 79 66.0 80 29.0 81 7.0 82 2.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016504492092195528 2 0.010763799190562301 3 0.015786905479491374 4 0.0 5 0.012438167953538658 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.783910751361023E-4 28-29 0.0010763799190562301 30-31 0.0011959776878402557 32-33 9.567821502722046E-4 34-35 0.002870346450816614 36-37 3.5879330635207673E-4 38-39 0.0 40-41 0.0031095419883846644 42-43 4.783910751361023E-4 44-45 3.5879330635207673E-4 46-47 2.3919553756805115E-4 48-49 0.0 50-51 0.0 52-53 1.1959776878402558E-4 54-55 0.0021527598381124603 56-57 0.0011959776878402557 58-59 1.1959776878402558E-4 60-61 0.0 62-63 0.0013155754566242812 64-65 0.001435173225408307 66-67 0.003587933063520767 68-69 0.007295463895825559 70-71 0.006697475051905432 72-73 0.014232134485299043 74-75 0.013753743410162939 76-77 0.015428112173139299 78-79 0.0019135643005444092 80-81 0.011122592496914378 82-83 0.010763799190562301 84-85 0.012557765722322683 86-87 0.017939665317603833 88-89 0.0037075308323047925 90-91 0.009328625965153993 92-93 0.014590927791651118 94-95 0.016265296554627478 96-97 0.01674368762976358 98-99 0.008491441583665816 100-101 0.04640393428820192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 418068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.780141029688949 #Duplication Level Percentage of deduplicated Percentage of total 1 68.07447866961766 8.019269592506483 2 8.696623281690998 2.048948974807926 3 3.727994477045219 1.3174890209248256 4 2.333042295275031 1.099342690662763 5 1.6934353997035474 0.9974453916587732 6 1.2203293467887673 0.8625391084703924 7 1.082255477268574 0.8924385506663989 8 0.8446872017705944 0.7960427490264741 9 0.7756502670104977 0.8223542581589598 >10 7.949399987817012 20.969076800903203 >50 1.89242421165912 16.073700929035468 >100 1.5980019898881197 35.734856530516566 >500 0.0913724136530691 7.262933302716304 >1k 0.020304980811793133 3.103562099945463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 2087 0.4992010869045227 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.41763540859381726 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1512 0.3616636528028933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 0.33917927227149647 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1117 0.26718141546351315 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1060 0.25354726982213416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.24350105724427604 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1009 0.2413482974061636 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1007 0.24086990633102748 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1001 0.2394347331056192 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 993 0.2375211688050748 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 940 0.22484380531396808 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 930 0.22245184993828754 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 898 0.21479759273610993 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 867 0.20738253107150031 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 866 0.20714333553393227 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 861 0.20594735784609203 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 836 0.19996746940689072 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 793 0.18968206129146456 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 790 0.1889644746787604 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 765 0.18298458623955913 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 765 0.18298458623955913 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 755 0.1805926308638786 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 752 0.17987504425117445 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 746 0.17843987102576614 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 742 0.17748308887549394 TruSeq Adapter, Index 1 (95% over 21bp) GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 738 0.17652630672522174 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 736 0.17604791565008562 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 733 0.17533032903738147 No Hit TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC 709 0.16958963613574823 No Hit GTGTTATGGCTGGGGGTTTCATGTTGATAATAGTGGTAATAAAATTAATT 702 0.1679152673727719 No Hit CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG 672 0.16073940124573036 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 640 0.15308514404355272 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 638 0.1526067529684166 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 626 0.1497364065176 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 616 0.1473444511419195 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 608 0.1454308868413751 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 604 0.1444741046911029 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 594 0.14208214931542237 No Hit ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT 562 0.13442789211324474 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 552 0.13203593673756422 No Hit GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC 550 0.1315575456624281 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 549 0.13131835012486007 RNA PCR Primer, Index 1 (95% over 23bp) GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 545 0.13036156797458787 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 542 0.1296439813618837 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 541 0.12940478582431567 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 525 0.12557765722322684 No Hit ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA 523 0.12509926614809075 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 514 0.12294650630997829 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 512 0.12246811523484219 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 512 0.12246811523484219 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 511 0.12222891969727412 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 505 0.12079374647186582 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 503 0.12031535539672972 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 503 0.12031535539672972 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 499 0.11935857324645752 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 498 0.11911937770888946 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 489 0.11696661787077701 No Hit CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA 480 0.11481385803266454 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 480 0.11481385803266454 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 475 0.11361788034482428 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 468 0.11194351158184794 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 467 0.11170431604427988 No Hit ATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCT 457 0.10931236066859937 No Hit GTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTC 454 0.10859477405589521 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 441 0.10548523206751054 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 436 0.1042892543796703 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 435 0.10405005884210224 No Hit GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC 434 0.10381086330453419 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 434 0.10381086330453419 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 432 0.10333247222939809 No Hit GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG 429 0.10261488561669393 No Hit CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA 424 0.10141890792885369 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 424 0.10141890792885369 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 421 0.10070132131614953 No Hit GTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTACTGC 421 0.10070132131614953 No Hit GTATAGTAATGCCTGCGGCTAGCACTGGTAGTGATAATAGGAGCAGTACG 420 0.10046212577858146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3919553756805112E-4 0.0 3 0.0 0.0 0.0 2.3919553756805112E-4 0.0 4 0.0 0.0 0.0 7.175866127041533E-4 0.0 5 0.0 0.0 0.0 7.175866127041533E-4 0.0 6 0.0 0.0 0.0 7.175866127041533E-4 0.0 7 0.0 0.0 0.0 7.175866127041533E-4 0.0 8 0.0 0.0 0.0 7.175866127041533E-4 0.0 9 0.0 0.0 0.0 0.0011959776878402557 0.0 10-11 0.0 0.0 0.0 0.0011959776878402557 0.0 12-13 0.0 0.0 0.0 0.0014351732254083067 0.0 14-15 0.0 0.0 0.0 0.001674368762976358 0.0 16-17 0.0 0.0 0.0 0.002750748682032588 0.0 18-19 0.0 0.0 0.0 0.003348737525952716 0.0 20-21 0.0 0.0 0.0 0.004783910751361023 0.0 22-23 0.0 0.0 0.0 0.007056268358257508 0.0 24-25 0.0 0.0 0.0 0.009807017040290097 0.0 26-27 0.0 0.0 0.0 0.012796961259890736 0.0 28-29 0.0 0.0 0.0 0.01805926308638786 0.0 30-31 0.0 0.0 0.0 0.024756738138293294 0.0 32-33 0.0 0.0 0.0 0.034444157409799364 0.0 34-35 0.0 0.0 0.0 0.051187845039562946 0.0 36-37 0.0 0.0 0.0 0.0729546389582556 0.0 38-39 0.0 0.0 0.0 0.11170431604427988 0.0 40-41 0.0 0.0 0.0 0.1614569878584345 0.0 42-43 0.0 0.0 0.0 0.2048709779270358 0.0 44-45 0.0 0.0 0.0 0.24912215237712526 0.0 46-47 0.0 0.0 0.0 0.30628988585588945 0.0 48-49 0.0 0.0 0.0 0.36513198809763003 0.0 50-51 0.0 0.0 0.0 0.4335419118420927 0.0 52-53 0.0 0.0 0.0 0.5027890199680435 0.0 54-55 0.0 0.0 0.0 0.5814843518279322 0.0 56-57 0.0 0.0 0.0 0.6614952591444454 0.0 58-59 0.0 0.0 0.0 0.7502368035821924 0.0 60-61 0.0 0.0 0.0 0.8477089851411732 0.0 62-63 0.0 0.0 0.0 0.9522374350584115 0.0 64-65 0.0 0.0 0.0 1.0640613488714754 0.0 66-67 0.0 0.0 0.0 1.1953796989963354 0.0 68-69 0.0 0.0 0.0 1.3445181166700153 0.0 70-71 0.0 0.0 0.0 1.5200876412449649 0.0 72-73 0.0 0.0 0.0 1.7005606743400596 0.0 74-75 0.0 0.0 0.0 1.8894055512500358 0.0 76-77 0.0 0.0 0.0 2.085545892055838 0.0 78-79 0.0 0.0 0.0 2.323784647473617 0.0 80-81 0.0 0.0 0.0 2.584986174497929 0.0 82-83 0.0 0.0 0.0 2.877283121406087 0.0 84-85 0.0 0.0 0.0 3.179865476429672 0.0 86-87 0.0 0.0 0.0 3.4738367921008066 0.0 88-89 0.0 0.0 0.0 3.813614053216223 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 30 1.3601111E-6 79.22243 2 GACATAT 170 0.0 78.2904 1 ACATATA 190 0.0 70.0493 2 GAGAGCC 60 3.2923708E-10 63.32488 8 CATATAG 220 0.0 60.489883 3 CATATGC 25 0.0050074486 57.03332 3 GCAGATC 65 4.4148692E-8 51.14702 8 TGGGTAT 95 7.2759576E-12 49.99333 6 CCCGCCG 20 5.3829345E-4 47.507866 74-75 TCGCCCG 20 5.3845224E-4 47.505024 72-73 GTCTCGC 20 5.3845224E-4 47.505024 68-69 CGCGCCG 20 5.3845224E-4 47.505024 80-81 TCTCGCC 20 5.3845224E-4 47.505024 70-71 CCGCGCC 20 5.3845224E-4 47.505024 78-79 GGCCCGT 20 5.389288E-4 47.496502 64-65 GCCCGTC 20 5.389288E-4 47.496502 64-65 AGTCCGC 20 5.3908775E-4 47.49366 42-43 CGGCCCG 20 5.3908775E-4 47.49366 62-63 GCGCACC 20 5.3908775E-4 47.49366 54-55 GCACCAC 20 5.3908775E-4 47.49366 56-57 >>END_MODULE