FastQCFastQC Report
Fri 10 Feb 2017
SRR2568607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230101
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT352.7799336E-454.298776
GGTATCA1305.456968E-1240.2019731
GTATCAA2100.036.1991841
CAGTGCG550.002572312534.5537649
GTTACTC600.003939788331.6742863
GTATATA902.4006777E-531.6742841
CAACGCA2300.030.9857145
GTGTAGG1102.7158148E-630.2345451
TCAACGC2250.029.5626684
CTATTAG650.005827615529.23781
TGCTAGT650.005827615529.23782
CTAGTGT1205.347112E-627.7154
GACTTAT700.0083680227.1493851
AATTCGC350.00837658627.14938578-79
GTATAGC700.0083680227.1493851
CCCATAC908.913154E-426.3952373
TAAGGCT1109.4288705E-525.9153254
GTACATG6300.025.6410881
ATCAACG2651.8189894E-1225.1003782
GTCTATA950.001221853725.0060141
Produced by FastQC (version 0.11.5)