##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568607_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 230101 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.468642031108075 34.0 31.0 34.0 31.0 34.0 2 32.59251372223502 34.0 31.0 34.0 31.0 34.0 3 32.752595599323776 34.0 33.0 34.0 31.0 34.0 4 35.88515043393988 37.0 37.0 37.0 35.0 37.0 5 35.89900956536477 37.0 37.0 37.0 35.0 37.0 6 36.02842664742874 37.0 37.0 37.0 35.0 37.0 7 36.085301671874525 37.0 37.0 37.0 35.0 37.0 8 36.11758749418733 37.0 37.0 37.0 35.0 37.0 9 37.98033906849601 39.0 39.0 39.0 37.0 39.0 10-11 37.993830969878445 39.0 39.0 39.0 37.0 39.0 12-13 37.98325300628854 39.0 39.0 39.0 37.0 39.0 14-15 39.57125566599015 41.0 40.0 41.0 38.0 41.0 16-17 39.56211402818762 41.0 40.0 41.0 38.0 41.0 18-19 39.55668597702748 41.0 40.0 41.0 37.5 41.0 20-21 39.54690766228743 41.0 40.0 41.0 38.0 41.0 22-23 39.48352028022477 41.0 40.0 41.0 37.0 41.0 24-25 39.434748219260236 41.0 40.0 41.0 37.0 41.0 26-27 39.36498320302823 41.0 40.0 41.0 37.0 41.0 28-29 39.2918783490728 41.0 40.0 41.0 37.0 41.0 30-31 39.21615507972586 41.0 40.0 41.0 36.5 41.0 32-33 39.12445404409368 41.0 39.0 41.0 36.0 41.0 34-35 39.04760518207222 41.0 39.0 41.0 36.0 41.0 36-37 38.947788145205806 41.0 39.0 41.0 35.0 41.0 38-39 38.85251693821409 40.0 39.0 41.0 35.0 41.0 40-41 38.73641357490841 40.0 39.0 41.0 35.0 41.0 42-43 38.63039056762031 40.0 38.0 41.0 35.0 41.0 44-45 38.49691005254215 40.0 38.0 41.0 34.5 41.0 46-47 38.500886567203096 40.0 38.5 41.0 34.5 41.0 48-49 38.674777597663635 40.0 39.0 41.0 35.0 41.0 50-51 37.17233519193745 38.5 37.0 40.0 32.5 40.5 52-53 37.375665468642026 39.5 37.5 39.5 33.5 40.5 54-55 38.18052072785429 40.0 38.0 41.0 34.0 41.0 56-57 38.13113806545822 40.0 37.0 41.0 34.0 41.0 58-59 37.91088913129452 40.0 37.0 41.0 34.0 41.0 60-61 37.63834576990104 39.5 36.0 41.0 34.0 41.0 62-63 37.31762573826276 39.0 35.5 41.0 33.0 41.0 64-65 36.989448111916076 39.0 35.0 41.0 33.0 41.0 66-67 36.82922716546213 37.5 35.0 40.5 33.0 41.0 68-69 36.47125175466426 37.0 35.0 40.0 33.0 41.0 70-71 36.090086527220656 37.0 35.0 39.0 33.0 41.0 72-73 35.71282176087892 36.0 35.0 39.0 32.0 41.0 74-75 35.32627194145179 36.0 35.0 38.0 32.0 39.5 76-77 34.95925050303997 35.0 35.0 37.0 32.0 39.0 78-79 34.61952142754703 35.0 35.0 37.0 32.0 39.0 80-81 34.33800591913986 35.0 35.0 36.0 31.0 37.5 82-83 34.09505608406744 35.0 35.0 36.0 31.0 37.0 84-85 33.882810157278755 35.0 34.5 35.5 31.0 37.0 86-87 33.725983372519025 35.0 34.0 35.0 31.0 36.0 88-89 33.6056036262337 35.0 34.0 35.0 31.0 36.0 90-91 33.470504256826345 35.0 34.0 35.0 30.5 36.0 92-93 33.374326926002055 35.0 34.0 35.0 30.0 35.5 94-95 33.297838775146566 35.0 34.0 35.0 30.0 35.0 96-97 33.20958187926172 35.0 34.0 35.0 30.0 35.0 98-99 33.16926045519142 35.0 34.0 35.0 30.0 35.0 100-101 31.852051925024227 34.5 32.0 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46377649246795727 1101 2 0.4676978928902287 1101 3 0.45178266402331246 1101 4 0.3969205786633552 1101 5 0.48559693500072143 1101 6 0.3243167300469736 1101 7 0.2692746558611816 1101 8 0.36042538377397904 1101 9 0.3403318809267688 1101 10-11 0.4383855464838433 1101 12-13 0.5475862994519289 1101 14-15 0.631216503505776 1101 16-17 0.7625597764009484 1101 18-19 0.637197084604189 1101 20-21 0.6624354239715942 1101 22-23 0.6460432431973189 1101 24-25 0.7010402415406531 1101 26-27 0.7446748441342521 1101 28-29 0.8227352449630061 1101 30-31 0.854829023486765 1101 32-33 0.6572916728628257 1101 34-35 0.6820652572694854 1101 36-37 0.8871835963682813 1101 38-39 0.7649869724817719 1101 40-41 0.8160179857200589 1101 42-43 0.9105262425032876 1101 44-45 0.7077638650120051 1101 46-47 0.9650193826913451 1101 48-49 0.6590345867975671 1101 50-51 0.8031322263338652 1101 52-53 0.809031724621903 1101 54-55 0.8668412408104516 1101 56-57 0.8934975119187882 1101 58-59 0.9221588923502111 1101 60-61 1.1096106656675886 1101 62-63 1.0946465093392916 1101 64-65 0.801203998620899 1101 66-67 0.7964060906026091 1101 68-69 0.9272984415201151 1101 70-71 0.9943696794990871 1101 72-73 0.8338438473027878 1101 74-75 0.7627230788316623 1101 76-77 0.8971387867659502 1101 78-79 1.0526586746522852 1101 80-81 0.9401662642178579 1101 82-83 0.7953973940052137 1101 84-85 0.6883672375679168 1101 86-87 0.8208759077506045 1101 88-89 0.6207339081544703 1101 90-91 0.602753132694211 1101 92-93 0.5751334728826407 1101 94-95 0.5242975903906242 1101 96-97 0.39882652709116684 1101 98-99 0.5017614428881387 1101 100-101 0.15552603244813312 1102 1 0.006211801641725856 1102 2 0.09578416813447888 1102 3 0.24801826660782922 1102 4 0.009087760359520303 1102 5 0.21718853221643997 1102 6 0.023633145441671388 1102 7 0.03463970252597903 1102 8 -0.03215194868536031 1102 9 0.26604277770237417 1102 10-11 0.04944463441342606 1102 12-13 0.5151394411318648 1102 14-15 0.2826819965294689 1102 16-17 0.3726044122907055 1102 18-19 0.35533548707632434 1102 20-21 0.28935117763143126 1102 22-23 0.3408180348444745 1102 24-25 0.39280289097723653 1102 26-27 0.48377338644470314 1102 28-29 0.2884779799257018 1102 30-31 0.2606722672361812 1102 32-33 0.5006290640043147 1102 34-35 0.33083074717178107 1102 36-37 0.16887351739709544 1102 38-39 0.3187529305927086 1102 40-41 0.23598552325478295 1102 42-43 0.09116113341067233 1102 44-45 0.3155923133685974 1102 46-47 0.31321053666955123 1102 48-49 0.3971498909771114 1102 50-51 0.11811178122352572 1102 52-53 0.22423914104665954 1102 54-55 0.21309713243576311 1102 56-57 0.45289758058938645 1102 58-59 0.33349578406915725 1102 60-61 0.28280103929807865 1102 62-63 0.38846303398267423 1102 64-65 0.07051760476380053 1102 66-67 0.3841952094339618 1102 68-69 0.13610388522582895 1102 70-71 0.24042892349821443 1102 72-73 0.10362566207945179 1102 74-75 0.19129004827325247 1102 76-77 0.28436293384589106 1102 78-79 -0.0396720679266096 1102 80-81 0.011271548732636916 1102 82-83 -0.025122629717351685 1102 84-85 0.03738556013245642 1102 86-87 0.02473394689284447 1102 88-89 0.17161257969050325 1102 90-91 0.46853332434761086 1102 92-93 0.2645394721959349 1102 94-95 0.02038024482144607 1102 96-97 0.045750396367310486 1102 98-99 -0.20709082168095705 1102 100-101 0.15232660678406162 1103 1 0.24334923258095387 1103 2 0.18547684769249173 1103 3 0.37727701430580396 1103 4 0.06102721708143122 1103 5 0.1630333756786868 1103 6 0.1506761933238039 1103 7 0.15725135077644126 1103 8 0.24213442332200685 1103 9 0.35374996002281733 1103 10-11 0.40130927529740035 1103 12-13 0.3111367938022127 1103 14-15 0.4069050628278106 1103 16-17 0.2863503837455852 1103 18-19 0.34910562980191884 1103 20-21 0.3454774437456152 1103 22-23 0.2851233985645436 1103 24-25 0.30798762854630013 1103 26-27 0.3269047170156014 1103 28-29 0.13196900202515138 1103 30-31 0.23225663648111095 1103 32-33 0.14122528868202977 1103 34-35 0.17412034201524307 1103 36-37 0.12063168958862747 1103 38-39 0.031229910334886313 1103 40-41 0.0773615591663841 1103 42-43 0.2717550560626094 1103 44-45 0.01929281981427522 1103 46-47 -0.0215183997945374 1103 48-49 -0.10270623241147803 1103 50-51 -0.4935220815757333 1103 52-53 -0.4601243488244222 1103 54-55 -0.14737132981101553 1103 56-57 -0.21292021406991068 1103 58-59 -0.12109675736730452 1103 60-61 -0.11602492755275051 1103 62-63 0.18151091611895964 1103 64-65 0.12826967658700283 1103 66-67 0.09438455105458132 1103 68-69 0.16741143587039886 1103 70-71 0.20146713712621533 1103 72-73 0.08574179928018566 1103 74-75 0.030378446063302533 1103 76-77 -0.104406057866818 1103 78-79 -0.07061992704263531 1103 80-81 -0.09492377815502806 1103 82-83 1.9965389222420526E-4 1103 84-85 -0.06022033305355734 1103 86-87 0.011598012270376046 1103 88-89 0.35007712849345296 1103 90-91 0.13347347167727008 1103 92-93 0.05981744180919435 1103 94-95 -0.4107747966150228 1103 96-97 -0.09870172149641121 1103 98-99 -0.24567852886327302 1103 100-101 -0.28091464551796363 1104 1 0.2263655369287818 1104 2 0.40490619551857776 1104 3 0.3347045505376869 1104 4 0.31487685476259486 1104 5 0.2912036655337644 1104 6 0.2836019179614908 1104 7 0.41291258266049624 1104 8 0.46043152477128046 1104 9 0.39179343828368474 1104 10-11 0.3089179709495724 1104 12-13 0.4924094387297444 1104 14-15 0.5122039758712944 1104 16-17 0.5056665069339914 1104 18-19 0.5242641442946763 1104 20-21 0.5059167553398112 1104 22-23 0.676993869579043 1104 24-25 0.6310175198506442 1104 26-27 0.4579056228127101 1104 28-29 0.4872679150686352 1104 30-31 0.667605368365173 1104 32-33 0.6661347089718817 1104 34-35 0.650116718826844 1104 36-37 0.6662612185741281 1104 38-39 0.5756139683058947 1104 40-41 0.6429186243837748 1104 42-43 0.47555940388869544 1104 44-45 0.5462402835823852 1104 46-47 0.6608864915098067 1104 48-49 0.8630999270088111 1104 50-51 0.6238918676996335 1104 52-53 0.8634716656683281 1104 54-55 0.5756812064208674 1104 56-57 0.8530897496981993 1104 58-59 0.8585228078500862 1104 60-61 0.8106055072298588 1104 62-63 0.7920181624957721 1104 64-65 0.7539490244130889 1104 66-67 0.695494152503862 1104 68-69 0.6991143344211181 1104 70-71 0.7644200356769346 1104 72-73 0.7969057485555524 1104 74-75 0.8157045330198258 1104 76-77 0.6880531812636193 1104 78-79 0.6464317033921461 1104 80-81 0.9131151711203387 1104 82-83 1.050018494471935 1104 84-85 0.9243811162218094 1104 86-87 1.0155608745892195 1104 88-89 0.9909285777688126 1104 90-91 0.8852850658801614 1104 92-93 0.719124507026585 1104 94-95 0.7627985729501958 1104 96-97 0.7128924814021431 1104 98-99 0.8392399493975944 1104 100-101 0.6629259341921774 1105 1 0.5522434633501874 1105 2 0.48475569384633843 1105 3 0.38528983481862156 1105 4 0.2897932427224603 1105 5 0.4891551705504824 1105 6 0.4876152958879061 1105 7 0.5991584020584924 1105 8 0.46168570537328435 1105 9 0.6518268830997371 1105 10-11 0.44217465991278715 1105 12-13 0.5312890373919572 1105 14-15 0.6220492935970441 1105 16-17 0.5786180119507094 1105 18-19 0.6257482580070501 1105 20-21 0.5375848155404839 1105 22-23 0.546141026769682 1105 24-25 0.5465693593155265 1105 26-27 0.5278261913745794 1105 28-29 0.3975940020251514 1105 30-31 0.5173127262247021 1105 32-33 0.6211932373999787 1105 34-35 0.7646652138101118 1105 36-37 0.5903832921527297 1105 38-39 0.4767427308477039 1105 40-41 0.8075298283971577 1105 42-43 0.5506012099087627 1105 44-45 0.6206549993014505 1105 46-47 0.5019191002054626 1105 48-49 0.3039444086141643 1105 50-51 0.3756686235524711 1105 52-53 0.3063019332268553 1105 54-55 0.34261264454795537 1105 56-57 0.4467352346480311 1105 58-59 0.2659224734019219 1105 60-61 0.20849430321647588 1105 62-63 0.11580578791382123 1105 64-65 0.11745236889469624 1105 66-67 0.023671410028946127 1105 68-69 0.31084092304988076 1105 70-71 0.36412739353646373 1105 72-73 0.03906711979301036 1105 74-75 0.0015322922171492337 1105 76-77 -0.025680096328358104 1105 78-79 -0.10367281165802211 1105 80-81 0.003834234665490044 1105 82-83 -0.0350567563641917 1105 84-85 -0.23489982023304634 1105 86-87 -0.007432436447565749 1105 88-89 -0.08902543560910914 1105 90-91 0.14228757424137228 1105 92-93 0.013543403347654248 1105 94-95 -0.112096911999636 1105 96-97 0.2370354579907712 1105 98-99 0.04398493267518688 1105 100-101 0.09488663653331386 1106 1 0.19809340356540872 1106 2 0.13989718448005561 1106 3 0.24180637527298643 1106 4 0.26328865922305766 1106 5 0.2507384706700506 1106 6 0.27926535567268473 1106 7 0.1973798050078699 1106 8 0.3281663749627626 1106 9 0.4243458149450987 1106 10-11 0.4353118659709807 1106 12-13 0.4880447169455593 1106 14-15 0.39629949287962773 1106 16-17 0.3515355089614687 1106 18-19 0.38295979646859024 1106 20-21 0.4830934843328336 1106 22-23 0.3896543787027156 1106 24-25 0.5317975378727198 1106 26-27 0.4524849199517078 1106 28-29 0.5076976289681525 1106 30-31 0.6052461658420896 1106 32-33 0.48929307797391175 1106 34-35 0.5898694784573877 1106 36-37 0.5267872379478646 1106 38-39 0.5003577168979234 1106 40-41 0.5494713389591297 1106 42-43 0.4301099783424007 1106 44-45 0.42573172309578666 1106 46-47 0.553570654609608 1106 48-49 0.3968350024762586 1106 50-51 0.4898949089251303 1106 52-53 0.6204833800184062 1106 54-55 0.6165265544722303 1106 56-57 0.5334231581235258 1106 58-59 0.5688319991093778 1106 60-61 0.3914232589757418 1106 62-63 0.49217585566990607 1106 64-65 0.6404944175196405 1106 66-67 0.5013766710891261 1106 68-69 0.5033354427788552 1106 70-71 0.37855047045954393 1106 72-73 0.4106419504028196 1106 74-75 0.3859483942498301 1106 76-77 0.531078074257529 1106 78-79 0.5974905004185089 1106 80-81 0.5151933418795167 1106 82-83 0.39614093195785216 1106 84-85 0.23079866521933212 1106 86-87 0.21255062453290208 1106 88-89 0.14320104904613373 1106 90-91 0.2365606910209621 1106 92-93 0.3348741127936492 1106 94-95 0.20131231478394085 1106 96-97 0.1520538916296701 1106 98-99 0.17425346595538116 1106 100-101 0.3896380315113923 1107 1 -0.2259094883492807 1107 2 -0.011398152307506848 1107 3 -0.015067946934507859 1107 4 0.12539252715895088 1107 5 0.020349267151559047 1107 6 0.10326824758737274 1107 7 -0.04679419185922029 1107 8 0.17653364812820627 1107 9 0.1322383321158398 1107 10-11 0.09404183343693973 1107 12-13 0.11948223953864101 1107 14-15 0.2739835511998976 1107 16-17 -0.2166413798203095 1107 18-19 -0.09378438957791957 1107 20-21 0.08211746312545642 1107 22-23 0.14099791406842854 1107 24-25 0.14283501226722706 1107 26-27 -0.03379053492238171 1107 28-29 0.19178004853677777 1107 30-31 0.24249161710126543 1107 32-33 0.4578435057362924 1107 34-35 0.15091297767416023 1107 36-37 0.017954848503350718 1107 38-39 -0.1945452834635688 1107 40-41 0.1945490591663841 1107 42-43 -0.1401635485885535 1107 44-45 0.07595464151969367 1107 46-47 0.01950630950778276 1107 48-49 0.07851954278232398 1107 50-51 0.0780501083467442 1107 52-53 0.16693475970270555 1107 54-55 0.10119940662308835 1107 56-57 -0.4012075202714698 1107 58-59 -0.12531187364637475 1107 60-61 -0.16689702057600897 1107 62-63 0.03519308666158594 1107 64-65 -0.07747160248276685 1107 66-67 0.03223581074450266 1107 68-69 -0.08065057963347755 1107 70-71 0.03203884255256639 1107 72-73 -0.020156941029888742 1107 74-75 -0.15857504230879016 1107 76-77 -0.08620111600635738 1107 78-79 0.07469257295736753 1107 80-81 0.166982714093038 1107 82-83 0.05640120427981543 1107 84-85 0.050632380124746135 1107 86-87 0.22568473707658399 1107 88-89 0.10671472539267057 1107 90-91 -0.00790249731497994 1107 92-93 0.052731685995240696 1107 94-95 -0.12214834312664635 1107 96-97 -0.11449629513982273 1107 98-99 0.012824378113471369 1107 100-101 0.07296132347428497 1108 1 0.280904057142358 1108 2 0.3184264090959985 1108 3 0.1568822774636942 1108 4 0.294853971467397 1108 5 0.33134477918746086 1108 6 0.18329680735889298 1108 7 0.3801131928817014 1108 8 0.22788003735708884 1108 9 0.43050434598772824 1108 10-11 0.3782829595079278 1108 12-13 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-0.8968140130984708 2316 62-63 -1.5223976384533202 2316 64-65 -1.2085205151534097 2316 66-67 -1.14227197549409 2316 68-69 -0.8753614254865383 2316 70-71 -0.8544878776230505 2316 72-73 -0.45631664467261146 2316 74-75 -0.7944740613113268 2316 76-77 -0.5828122156839228 2316 78-79 -0.4497912028538451 2316 80-81 -0.36149365278629375 2316 82-83 -0.42089179153550305 2316 84-85 -0.30063331240458524 2316 86-87 -0.0334563756352253 2316 88-89 -0.16845163256849816 2316 90-91 -0.17865779675931748 2316 92-93 -0.21265213783681958 2316 94-95 -0.5817971048746102 2316 96-97 -0.5273521639742853 2316 98-99 -0.514437747152364 2316 100-101 -0.05303853932327485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 306.0 3 24.0 4 12.0 5 2.0 6 32.0 7 158.0 8 155.0 9 84.0 10 70.0 11 98.0 12 122.0 13 137.0 14 167.0 15 227.0 16 230.0 17 268.0 18 321.0 19 356.0 20 418.0 21 462.0 22 538.0 23 581.0 24 696.0 25 750.0 26 904.0 27 985.0 28 1172.0 29 1429.0 30 1821.0 31 2289.0 32 3017.0 33 4246.0 34 6556.0 35 11474.0 36 25781.0 37 63485.0 38 85220.0 39 15507.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.271198843145115 15.695506733801418 10.845079011098141 26.18821541195533 2 16.834723522878235 18.247272252107063 38.45155389071586 26.46645033429884 3 20.8328069663401 21.618219122586154 27.428003885677445 30.1209700253963 4 13.193418972073756 14.272833004456176 35.79272462745406 36.74102339601602 5 14.767772594422013 36.549627364633835 34.4135620979088 14.269037943035357 6 36.23080474187114 33.853771982544615 16.37705017986708 13.53837309571716 7 32.00339706458778 29.777884238491353 19.0989068420365 19.119811854884368 8 28.374635251077912 34.36174382648839 18.507469186882105 18.756151735551587 9 27.590531977440495 14.434597042876119 17.551553687694618 40.423317291988766 10-11 27.030134373305636 24.43542960916116 27.155122669262244 21.37931334827096 12-13 27.27389525350429 21.456024632912552 26.737554521441652 24.532525592141507 14-15 24.028013083224813 23.02651922633021 24.138419124852465 28.807048565592513 16-17 24.905096079336193 26.05417359581378 24.439283257729446 24.60144706712058 18-19 24.714327379734545 25.69268808685318 25.426713273927938 24.166271259484333 20-21 25.51498545091288 24.846184482859933 25.259478670407137 24.37935139582005 22-23 24.995864062622445 24.9383963011659 24.986503783294296 25.079235852917357 24-25 24.8372743065382 24.821600393589367 25.380854315333007 24.960270984539427 26-27 25.037770105233825 25.13268518236321 25.08718689997779 24.742357812425166 28-29 24.932188557172775 25.158371828753793 25.123976297560528 24.785463316512903 30-31 25.104926768952122 25.17872381184584 24.999346928823947 24.717002490378086 32-33 24.748033244374014 25.061930489827727 25.132024049492145 25.058012216306114 34-35 25.19866706425473 25.100068126859615 25.01605225526024 24.685212553625416 36-37 25.219402754475638 24.999183971902518 25.059896462354992 24.721516811266845 38-39 25.15007334984612 25.166832810235025 25.074111639771722 24.608982200147132 40-41 25.435694761216638 25.02655082960461 24.94689834079078 24.59085606838797 42-43 24.947338642990815 24.77608042825434 25.30051790921356 24.976063019541282 44-45 25.121901864919028 25.187612464125714 24.873420062620895 24.817065608334367 46-47 24.94445786304756 25.092642761243884 24.975356916709643 24.987542458998917 48-49 24.91007487776059 25.03824401750412 25.128549947883688 24.923131156851607 50-51 24.992111625396866 25.325490656900314 24.914207125867993 24.768190591834824 52-53 24.995320858524526 25.142224137405712 25.070404756619354 24.792050247450412 54-55 24.930515804525776 25.00495149709331 25.16339940407916 24.901133294301754 56-57 24.827788841369856 25.19734909079038 25.18777273568172 24.787089332158054 58-59 24.958861172783074 24.971703452178833 25.260328239954728 24.809107135083366 60-61 24.9517805284767 25.023619921716833 25.235654979340733 24.788944570465738 62-63 24.84007113270341 25.03553370205671 25.10997490330262 25.014420261937254 64-65 24.79665683596513 25.012787167125555 25.30923179052988 24.881324206379436 66-67 24.53674788857141 25.125837009246542 25.176324144819738 25.16109095736231 68-69 24.833923368662923 25.0954682240408 24.98101515088875 25.08959325640752 70-71 24.794404583923622 24.78112983358686 25.1924294751686 25.232036107320916 72-73 24.614162604456823 24.946683495821727 25.256789693593312 25.18236420612813 74-75 24.714370308445233 25.153917829214343 25.18896842398593 24.942743438354494 76-77 24.60074539137401 25.015891813756653 25.162836666985953 25.220526127883385 78-79 24.718861380426002 24.81161263071721 25.275804334052555 25.19372165480423 80-81 24.667125303701898 25.138897355289856 25.21139393728284 24.98258340372541 82-83 24.688742932902578 24.955861255216643 25.387778738078186 24.967617073802593 84-85 24.805119888546646 24.859975837260688 25.239831513871806 25.095072760320864 86-87 24.525800996389233 24.963924963924963 25.394213629507746 25.116060410178058 88-89 24.604399215653842 24.79069230953381 25.378734295122214 25.226174179690137 90-91 24.671916581622106 25.058370669527356 25.228532323098246 25.04118042575229 92-93 24.532584191422877 24.88619251960622 25.517247382210346 25.063975906760554 94-95 24.48133141258035 24.927210135308634 25.508441013008603 25.083017439102413 96-97 24.318236228698282 24.787254880623326 25.65542908133284 25.23907980934555 98-99 24.309043585748235 24.749732322396998 25.605647779799263 25.3355763120555 100-101 24.52025215059381 24.696348343955698 25.545702295127644 25.23769721032285 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 289.0 1 149.0 2 6.5 3 7.0 4 8.5 5 6.0 6 6.5 7 9.5 8 9.0 9 7.0 10 7.0 11 9.5 12 11.5 13 9.0 14 8.5 15 9.5 16 12.0 17 17.0 18 20.0 19 20.5 20 18.5 21 19.5 22 28.5 23 36.5 24 43.5 25 56.5 26 74.0 27 105.0 28 142.0 29 188.0 30 236.5 31 319.0 32 465.0 33 634.0 34 876.0 35 1245.5 36 1809.5 37 2514.0 38 3286.0 39 4156.0 40 5113.0 41 6073.5 42 6922.0 43 7823.0 44 8597.0 45 9198.0 46 9829.5 47 10380.0 48 10944.5 49 11427.0 50 11552.0 51 11510.0 52 11422.0 53 11327.5 54 11117.5 55 10562.0 56 9784.5 57 8852.5 58 7903.5 59 6959.5 60 5933.0 61 4861.0 62 3841.0 63 2959.5 64 2151.5 65 1509.0 66 1117.5 67 879.5 68 706.0 69 527.0 70 396.5 71 308.0 72 213.5 73 167.0 74 138.0 75 96.5 76 80.0 77 60.5 78 36.5 79 22.5 80 17.0 81 13.5 82 7.0 83 4.5 84 4.5 85 3.0 86 3.0 87 2.5 88 2.0 89 2.0 90 1.5 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22294557607311574 2 0.2242493513717889 3 0.23467955376117441 4 0.23120281963137929 5 0.2259877184366865 6 0.21164619015128142 7 0.213384557216179 8 0.213384557216179 9 0.211211598385057 10-11 0.20838675190459843 12-13 0.2127326695668424 14-15 0.2146883325148522 16-17 0.17166374765863687 18-19 0.1651448711652709 20-21 0.15710492349011956 22-23 0.1768788488533296 24-25 0.1825285418142468 26-27 0.18339772534669557 28-29 0.1825285418142468 30-31 0.1812247665155736 32-33 0.17731344061955404 34-35 0.16623135058083188 36-37 0.14319798697093883 38-39 0.1651448711652709 40-41 0.15341089347721218 42-43 0.14384987462027543 44-45 0.13276778458155333 46-47 0.13928666107491927 48-49 0.14189421167226565 50-51 0.14450176226961203 52-53 0.1555838523083342 54-55 0.16188543291858792 56-57 0.16014706585369035 58-59 0.16992538059373927 60-61 0.18339772534669557 62-63 0.16927349294440266 64-65 0.1644929835159343 66-67 0.14754390463318282 68-69 0.13580992694512412 70-71 0.14841308816563162 72-73 0.14819579228251942 74-75 0.18817823477516396 76-77 0.18383231711291997 78-79 0.19752195774898848 80-81 0.18904741830761274 82-83 0.18578798006092975 84-85 0.1775307365026662 86-87 0.16145084115236352 88-89 0.15449737289277318 90-91 0.13885206930869487 92-93 0.14211150755537785 94-95 0.14341528285405106 96-97 0.15906058643812934 98-99 0.1508033428798658 100-101 0.17253293119108565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 230101.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.04225483400066 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77844508568397 68.70636351064331 2 11.07175831121071 17.94560517035206 3 2.520732643141403 6.1285757170154875 4 0.8330714259762321 2.7005594719556045 5 0.34909027826731875 1.4145531645706124 6 0.16697092087815618 0.8119019951805176 7 0.09445493211051696 0.5358388474900123 8 0.049729983893392705 0.3224184022063264 9 0.03559942365855463 0.25965518076721233 >10 0.099608793404596 1.1492306302794657 >50 5.382017751366772E-4 0.0252979095393881 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 2.1729588311219855E-4 0.0 30-31 0.0 0.0 0.0 0.0017383670648975884 0.0 32-33 0.0 0.0 0.0 0.00760535590892695 0.0 34-35 0.0 0.0 0.0 0.012168569454283119 0.0 36-37 0.0 0.0 0.0 0.018687445947649074 0.0 38-39 0.0 0.0 0.0 0.03585382071351276 0.0 40-41 0.0 0.0 0.0 0.06823090729723034 0.0 42-43 0.0 0.0 0.0 0.09408911738758197 0.0 44-45 0.0 0.0 0.0 0.1205992151272702 0.0 46-47 0.0 0.0 0.0 0.15514926054210976 0.0 48-49 0.0 0.0 0.0 0.1955662948009787 0.0 50-51 0.0 0.0 0.0 0.24684812321545757 0.0 52-53 0.0 0.0 0.0 0.29682617633126324 0.0 54-55 0.0 0.0 0.0 0.34158912825237614 0.0 56-57 0.0 0.0 0.0 0.4082989643678211 0.0 58-59 0.0 0.0 0.0 0.48674277817132483 0.0 60-61 0.0 0.0 0.0 0.5775724573122238 0.0 62-63 0.0 0.0 0.0 0.6857858071020987 0.0 64-65 0.0 0.0 0.0 0.7809614039052416 0.0 66-67 0.0 0.0 0.0 0.9076449037596535 0.0 68-69 0.0 0.0 0.0 1.0534504413279386 0.0 70-71 0.0 0.0 0.0 1.2272871478176974 0.0 72-73 0.0 0.0 0.0 1.4217669632031151 0.0 74-75 0.0 0.0 0.0 1.611683565043177 0.0 76-77 0.0 0.0 0.0 1.8168108787010921 0.0 78-79 0.0 0.0 0.0 2.0786524178512913 0.0 80-81 0.0 0.0 0.0 2.389168234818623 0.0 82-83 0.0 0.0 0.0 2.7248903742269697 0.0 84-85 0.0 0.0 0.0 3.0866880196087805 0.0 86-87 0.0 0.0 0.0 3.431319290224727 0.0 88-89 0.0 0.0 0.0 3.8350550410471924 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 35 2.7799336E-4 54.29877 6 GGTATCA 130 5.456968E-12 40.201973 1 GTATCAA 210 0.0 36.199184 1 CAGTGCG 55 0.0025723125 34.553764 9 GTTACTC 60 0.0039397883 31.674286 3 GTATATA 90 2.4006777E-5 31.674284 1 CAACGCA 230 0.0 30.985714 5 GTGTAGG 110 2.7158148E-6 30.234545 1 TCAACGC 225 0.0 29.562668 4 CTATTAG 65 0.0058276155 29.2378 1 TGCTAGT 65 0.0058276155 29.2378 2 CTAGTGT 120 5.347112E-6 27.715 4 GACTTAT 70 0.00836802 27.149385 1 AATTCGC 35 0.008376586 27.149385 78-79 GTATAGC 70 0.00836802 27.149385 1 CCCATAC 90 8.913154E-4 26.395237 3 TAAGGCT 110 9.4288705E-5 25.915325 4 GTACATG 630 0.0 25.641088 1 ATCAACG 265 1.8189894E-12 25.100378 2 GTCTATA 95 0.0012218537 25.006014 1 >>END_MODULE