FastQCFastQC Report
Fri 10 Feb 2017
SRR2568607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230101
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC301.2995533E-463.328123
TTCGCAT250.001615462737.9968732-33
TACATGG6350.035.9025542
GTACATG6800.034.923591
TACTAGA550.002576397734.542612
GTATAAT702.049241E-433.9257771
TACAGTA753.0723415E-431.6640597
ACATGGG7050.030.9903563
GGTATCA1401.6947524E-830.53321
GCCATAT804.4847623E-429.6850571
CATGGGG5150.029.5121334
GTCCTAA700.00838123927.1406211
GTTAGAC700.00838123927.1406213
ATAGAGC700.00838123927.1406218
CATTAGG700.00838123927.1406212
TATTAGG700.00838123927.1406212
GTGTAGC1405.7763646E-727.1406211
AGTACTC908.930021E-426.3867155
GTATCAA2207.6397555E-1125.9069581
TATACTG1109.44966E-525.9069585