FastQCFastQC Report
Fri 10 Feb 2017
SRR2568606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273903
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.042.333391
GTATCAA30200.035.5652351
TCAACGC29200.034.0148434
ATCAACG30150.033.1021463
CAACGCA31850.031.182665
AACGCAG32900.030.4736586
CGCAGAG35300.027.86468
ACGCAGA36350.027.0597067
TATCAAC38100.026.9434362
GTACATG60800.026.7329371
TACATGG65800.024.1959742
ACATGGG59600.024.1613143
CATGGGG29050.021.4303934
GCAGAGT48100.020.2519059
ACCGTCC1656.307894E-520.1591058
CATGGGA25800.019.3408094
GTATAAA15500.019.3167021
GTATATA18000.018.2180021
AGAGTAC42550.017.3096910-11
TATACAG21700.017.29995
Produced by FastQC (version 0.11.5)