FastQCFastQC Report
Fri 10 Feb 2017
SRR2568606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273903
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.038.8619461
TCAACGC28000.035.9640854
GTATCAA30450.034.3395081
ATCAACG29450.034.2001273
CAACGCA30000.033.566485
AACGCAG31650.032.1146056
GTACATG63050.027.2132721
TATCAAC38100.027.1872942
ACGCAGA37450.026.8872627
CGCAGAG37350.026.8320838
ACATGGG59850.025.4017123
TACATGG67650.024.3722782
GTACGGT1050.002197790722.6174326
CATGGGG29800.022.4747074
GCAGAGT46550.021.3250059
TATACCG1353.797664E-421.1109925
GTAGCGA1150.003725926220.6506986
GTACCGT1656.329682E-520.1500746
GTATATA19250.019.9992141
TATACTG20000.019.2373925
Produced by FastQC (version 0.11.5)