FastQCFastQC Report
Fri 10 Feb 2017
SRR2568605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488204
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15450.042.1410641
TCAACGC17850.034.3416374
GTACATG48950.033.8833351
CAACGCA18250.033.5889475
ATCAACG18450.033.2281953
GGTATCA9600.032.6727831
TACATGG50800.032.1816372
ACATGGG52550.030.0247083
AACGCAG20850.029.85326
TATCAAC21250.029.2971782
TTTCGCG350.008381234527.15111212-13
ACGCAGA24250.025.2757247
CATGGGA20100.024.3506584
CATGGGG34750.024.2040464
CGCAGAG26450.023.1733958
GAGTACA34950.022.7081991
AGTACAT33550.022.6642252
GTGTACG1754.0328287E-621.7252791
GTATATA6300.021.1217981
CGTATAG1951.0284757E-519.4970461
Produced by FastQC (version 0.11.5)