FastQCFastQC Report
Fri 10 Feb 2017
SRR2568605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488204
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.044.66341
GTATCAA18150.038.7444041
TCAACGC19900.034.369464
CAACGCA19950.034.0452465
GTACATG49550.032.6990131
ATCAACG21550.032.1863023
AACGCAG21700.031.2975586
TACATGG51850.031.154792
TATCAAC23800.029.5446912
ACATGGG53500.029.2151173
ACGCAGA26250.025.6917217
CGCAGAG27350.024.6584178
GAGTACA35450.023.5895861
CATGGGG35450.023.5808684
GTATATA5800.022.9379141
AGTACAT36150.021.94842
TTATACT7050.019.5376454
CATGGGA22000.019.214574
GCGTACA1507.71044E-419.0044212
ATGGGGG18550.018.1793275
Produced by FastQC (version 0.11.5)