FastQCFastQC Report
Fri 10 Feb 2017
SRR2568602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGTAGGGTTTCTCTCCAGTATGTATTCTTTCATGGTTTTTAAGAAG10.7407407407407408No Hit
CAATATCTGCTTCTCTGATCATTTCATCTACTTCTTCATCTGTTAGCTTT10.7407407407407408No Hit
GATCCTTAGTAGGCGCCTCCTGGGGTTTCCAAAGAAACAAAGAAAATTAG10.7407407407407408No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCCGCGCTTGTTTTTTTAA10.7407407407407408No Hit
GTACATGGGAAGCAGGGAAATCAACTCAGAAGCCATTACATTATAAGAGC10.7407407407407408No Hit
GTGTAGCCTTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCAGT10.7407407407407408No Hit
GTTGCACAGTATGATCAGAATGGCTGGAATGGAGAATGTGTATAATCTAA10.7407407407407408No Hit
TACCATGACTCTACAAGTACATTTTAAAAGACTGAAATGCACATTTTGAA10.7407407407407408No Hit
CCTTTCTTCTGTCATGGGACATCTAGGTTGTTTCCAGCTTCTAACTATCA10.7407407407407408No Hit
ACCCAACAGAACTGGAGTGTGTATCTACAGGGAGCTAGGACCTTGTGCTT10.7407407407407408No Hit
TATAAATGCTGCCGTTGTCAAACACCTGCTGGATGATCAGCCCGAAGGAG10.7407407407407408No Hit
GGATTCGGAGTCACTGCTCCCAGAGTAGGCGCCCAAGCCAGGGAGGATGC10.7407407407407408No Hit
GATCCTGAGGCCTGTGAGAAGCTGGAAGCCACTGTACTCCGACTGCGAAG10.7407407407407408No Hit
CTCCCACACAGGCGGCCACTGTGGTGGCTGTTGCCACTGAACTCCTAGAA10.7407407407407408No Hit
GCTCAGAGGCACTCAGGATGGTCCAGCGTTTGACATACCGCCGTAGGCTT10.7407407407407408No Hit
GGGGTACACCACGTGTGCGGTCCCTCCGGCGCCGAGTCAGGGGCTCTCGT10.7407407407407408No Hit
GTAGAAGTACTCCAATGTTTCAGGTTTCTATAAATGTATATGATCCATAA10.7407407407407408No Hit
GGGTGTCACTAGACTGGTACTCTCCCCACACAGTGTCCCCCTCCTGACTT10.7407407407407408No Hit
GTATTAGTTCAGGCAGATGCCCTGGTTCTGCGATCCCCTCTCCCTATAGG10.7407407407407408No Hit
TCCATGGTCACACAGACATTGTCTTGGCCCTGGATGTGTTCCGGAAGGGG10.7407407407407408No Hit
GGATGGTAAAGTCTGAACAACTTTATTAGTCAGCTTATTAGCACTCGTTA10.7407407407407408No Hit
CATTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACTTTGCCAGCTCC10.7407407407407408No Hit
ATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTT10.7407407407407408No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10.7407407407407408No Hit
TACTTGGAAAAGAGGCTACTAAGCAGAGACACCTGCGGTTACTTGGTTAC10.7407407407407408No Hit
GCCTACTAGGCATTGTATGAAGAGGATGACACACTGCCCCCGTGGCCCGT10.7407407407407408No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7407407407407408No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.7407407407407408No Hit
CTCTAAGAGACTGAAGGAAAGGAGGTCAAATGACCAGCCCAACTTGTTAT10.7407407407407408No Hit
TCACAGACCCTGGCCTTCTCCCAATGACTCTAGTCTCTAAGGAATGACTG10.7407407407407408No Hit
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA10.7407407407407408TruSeq Adapter, Index 1 (96% over 28bp)
CCTTTGTACTATGTCCCACAGGATACATTACCATGTCGCTTCCCATCATG10.7407407407407408No Hit
GAATAGTATTAGGAAATACAAGATAAGAGTCCCAAGTGATGAGGCCAAAG10.7407407407407408No Hit
CAGGGGCTGTGACCGCCGAGGTGCCTCCGCTTCTGCTGCTGCTAACGCGC10.7407407407407408No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
TCATTAAGTTGGACTAAGCGACCTTGAATGGATTCGACTGACTACCACCA10.7407407407407408No Hit
CCTTAGGAGCTGAGAGGGCCGCAGCTTCAGTGAGGCTGAGCAGCACTCTG10.7407407407407408No Hit
CTGGTGAAGGGTCCATACAGCTCGGCGTCCCCCGTGTTCTCCCATTTATA10.7407407407407408No Hit
GAGAAGCGGTATCTCCGGGCACTAGGCCTTGCCATCTCTCTGGATCGTCC10.7407407407407408No Hit
CCTTTTATGACATTAACTCAGCAGTACCAGAAACATCTCTAACTTGGCAA10.7407407407407408No Hit
CTCCCGAATCATTCTCTGGAAGGATGTGACAGAAGCTGAGCAGGCAGAAG10.7407407407407408No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA10.7407407407407408RNA PCR Primer, Index 31 (96% over 29bp)
GGACCAGACTCAAGACACACAACTCATAACAGTTGATGAAAAATTGGATA10.7407407407407408No Hit
GACTTATACAGATGGAGATCCAGGGGCGTCTCTAAGGAGCAGCATGGAGT10.7407407407407408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG10.7407407407407408RNA PCR Primer, Index 31 (96% over 29bp)
GTTTAATCAAATCCTGGTCTTTTGTGGATATTGTCTTGTTAAAAGCATCA10.7407407407407408No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCCCTCTT10.7407407407407408No Hit
GGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAAT10.7407407407407408No Hit
GTTCTGACACAGTGACCGCATGACGGTCCCCAGTTCCTTGGTTGTGATGG10.7407407407407408No Hit
CCCCAGTACCATACTGGGTTTCCATTCTATAAAATGTATTTATTCCTAAA10.7407407407407408No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATTTATATCAAAGTTCCCTTTACATTGTATTGAATAGACCCAAAAAGAAC10.7407407407407408No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCTC10.7407407407407408No Hit
CATGGGGACTGCTGGTGGATGGGCAGCGCGTCCGTCTGCCACGGTCCGCT10.7407407407407408No Hit
ACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACA10.7407407407407408No Hit
GCTCAGAACACTGGTCTAGGACCCGAGAAGACCTCCTTCTTCCAGGCTTT10.7407407407407408No Hit
GAGTTAGACTTCAAGAAATATCAATTTAGTACAGTTTGAGAAGTTGCAGG10.7407407407407408No Hit
TTCTGGTGTAAATTACTTGGGCTCTGTGCTTTGAAACCAGCTTGATAAGT10.7407407407407408No Hit
GCCATGGTGCGAGCGAAGGAAGGAAGAACGGAGCAGGCGAGGGTGGCTGC10.7407407407407408No Hit
CCCTTGATGTATTTGAGGCAGTCGACTCGTTTCTGAGACAGCATGAGATC10.7407407407407408No Hit
AGACCTATGAGCCTGTCTCCTATTATTTAGTTATTTTTAACGGTGAGCCA10.7407407407407408No Hit
GAACGGGCACGGGGAGGGGTCTGCACCTATTCTGAAGAAGGAGCCATAAA10.7407407407407408No Hit
CATTTCATGTCCTCTAGGAAAAACTGAGCTTCTTCTCTAGCAGTCTGAAA10.7407407407407408No Hit
CAAGGGTTGCATCCTATAGCAGTTTCTTACAATGTTTTCGACACAGTCCC10.7407407407407408No Hit
ATGGTGCTGAGTGGGAAGTATAAATTCGGGGAACAGATTCTTCGCCTTGC10.7407407407407408No Hit
AAAAGCACACTGCTAGCAGATGATTTAGAAGTTAAGTTGTTTGAATCAAG10.7407407407407408No Hit
CAGCTGAGGAGGGTAAATATGATTGCCTTATGAATCAGAAGTCATTCCTG10.7407407407407408No Hit
AGGTGGCTTCATCCCATTCGTCGACAGCGAGGGGAAGAGAGTGAGGAGTT10.7407407407407408No Hit
CTCCATATGCTGAGATGAATATGCTTTCAGTACTCTGGATCAGGTTAGCT10.7407407407407408No Hit
ATCAACAACCCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTG10.7407407407407408No Hit
TGTTTGGTGCTAGAAGAGGGCTCCAGACCTGCTAGAACTGGAGTTATAGA10.7407407407407408No Hit
ACCAAGGTAAGCAGTGGTTCCCGCTGTGCTTTCTCTGCATCATCCAGCTC10.7407407407407408No Hit
ATTCAAAATGGTGATGAATGCTATAGATAGTGTATGTGACTTGCTGAGCT10.7407407407407408No Hit
ATGTAGACAAATGCTAGAGTAAAAGTAAGATGGTCTCTGAATGTTGGAGT10.7407407407407408No Hit
GTTCACTACTCCTGAGGAAAGCTAGAGGAAGAAGAACCTTAGAGACGTCA10.7407407407407408No Hit
CTCTGGATCGTCCCCACACTGTGCTGACCGTTATCCAGGCCATCCGGAGA10.7407407407407408No Hit
CTGGATGATCATGCCATCACCGGTGGCAGTGACTCCCGAATCATTCTCTG10.7407407407407408No Hit
ATATTCATGTGCCCCGGGACCATCTGGCTTACCGCTATGAGGTGCTGAAA10.7407407407407408No Hit
CTGGAATATCATCGAGGGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
CATCTAATGAGAGAGAGGAGAGATGGAGGACCCAGGTTCTCCATCTTCAG10.7407407407407408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTG10.7407407407407408No Hit
GATCTCAAGTCTGAGGTTTACCCCTGCTGTCTTCTCTTCCAGCCTCCTGC10.7407407407407408No Hit
GGTATCAACGCAGAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
CCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAA10.7407407407407408RNA PCR Primer, Index 31 (96% over 29bp)
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTACAAAAAAAAAA10.7407407407407408No Hit
CCTTAGTGTTGTGGAGACCTCTTAGAGGTCACCTGCCCCCAAGGGAGACA10.7407407407407408No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA10.7407407407407408RNA PCR Primer, Index 27 (100% over 28bp)
GTTTAGAGCATCAATACTTAGGTTTTGAAACTTCTCAGTAATCATTGTCT10.7407407407407408No Hit
CCTCCAGGAACACTGGCCAGCGAGCTGTGCTGAAGTTTGCTGCTGCCACA10.7407407407407408No Hit
GTGCCCGCCAGAAGTATGTCCGAAAAGCTTGGGAGAAGGCGGATATCAAT10.7407407407407408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
TCCCAATACACCTGAAAAGCATGATGCTGACCTAAAATACCATTTCATGA10.7407407407407408No Hit
GTCCTAGACTGTGGTAGAGTCCATGATTGAGGTTCCTGGGACATTCCCAG10.7407407407407408No Hit
ATTCTAGACACTGTAATACTCAATAGCATTGTCAATAAAACAAGGACAAA10.7407407407407408No Hit
GTCTGTATGTGTGCGTGGGGGTGTTGCCTGAGCCTCTGAGAAAACACTTC10.7407407407407408No Hit
TTCCATTCCCATTCAGATCATCGTTTTCTGGGCACCGTAGAAAAAGAAGC10.7407407407407408No Hit
ATTAGGAATCTTTTATTTGTACCAGCAAGAGGACACTTCATCCAGCCCAT10.7407407407407408No Hit
CTCAGAGACCACGGCTGGAGTAATAAGCTTATAGTTCGGAACCTCCTTAC10.7407407407407408No Hit
GTTTCACCGTGATATCCAGCTGCATTAAGTGCACGGTGTTCGGCACCTCC10.7407407407407408No Hit
ATGCTACACATTGGGAGCACATCCATGTCGGGTTGGAAAAGACAGTCCTC10.7407407407407408No Hit
GAGTACATGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTC10.7407407407407408No Hit
TATCAACGCAGAGTACTGTTTTACTAAATAAATGTCTTGAGTGTTCCCAG10.7407407407407408No Hit
AGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.7407407407407408TruSeq Adapter, Index 1 (96% over 28bp)
TCAGTCAGCTGATCAGCCATGGTGCGAGCGAAGGAAGGAAGAACGGAGCA10.7407407407407408No Hit
AAGTAATATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAGAC10.7407407407407408TruSeq Adapter, Index 15 (96% over 27bp)
GTTCAGGTGTATTTTAGCAAAAACTAGTGGATGCCCTAACTTAGATGGTT10.7407407407407408No Hit
GTATCAACGCAGAGTACATGGGCCACTGCTGACGACACAGAGGCGGAGCT10.7407407407407408No Hit
GACCCTGACAGGCAAGACCATCACCCTGGAGGTCGAGCCCAGTGACACCA10.7407407407407408No Hit
GTATTAATCCAGGCCAAGTGGGTAAACTGAGAGTCAACAGAGCATGCTAC10.7407407407407408No Hit
AGATGGTACAGTTACTCAAGTGATTTTTACCTTGTCATGAGATTACTGTA10.7407407407407408No Hit
CACGGGGCGATGGCACCAGCCCGAGCAGCAGCTGGCACCTTATTGGCCAA10.7407407407407408No Hit
GTTCATGCATCTTCGCCAGCAGCTGGAGCGTCCCCACCCTTTGTGTTTCT10.7407407407407408No Hit
GGTTCTGACCCAGTGACCGCATGACGGTCCCCAGTTCCTTGGTTGTGATG10.7407407407407408No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
CTCCACCAGTGATCACACTGCCCAGAGGAACCAGTGAAGGGAGGAGAAAC10.7407407407407408No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT10.7407407407407408No Hit
GAACTAGATCTAACAAGTCCTTAGGATGGACCGTTCTTTAATCAGGTTTT10.7407407407407408No Hit
CATCTCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT10.7407407407407408RNA PCR Primer, Index 31 (96% over 28bp)
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTTTGCTTGGA10.7407407407407408RNA PCR Primer, Index 27 (96% over 28bp)
TGATGGTGAAGAAATCCTGTCATCCACTTTCCATCACCGTGGTCCTCGAG10.7407407407407408No Hit
AGCTCAGCCTTCCCATACTCCAGAGAATAGGAGGCTGACACAGTGGGGCC10.7407407407407408No Hit
GAATACAGGATAAGAAAAAAACCAAACCAAACCAAACCAAACAAAAAAAA10.7407407407407408No Hit
GCTCTATCTCCATCTCTAAAAGACGGTGGGGAATTAATTCTATTTACTGT10.7407407407407408No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAATAAGG10.7407407407407408TruSeq Adapter, Index 25 (96% over 27bp)
CCCTTGTCCTGTCTTAACTATATAGACCTGTCTGTAGTTTGTTTCACAGA10.7407407407407408No Hit
ACACATCTCCGAGCCCACGAACGCTACGCTATCTCGTATGCCGTCTTCTG10.7407407407407408RNA PCR Primer, Index 38 (95% over 24bp)
TAAATATGGTAGCTGGGGAGTAAAACCGAGCCATTTCAAACAGAAGACGG10.7407407407407408No Hit
GAATACAGAAAAGACTATATCCAGAAAGACCTTTTCACTCTGCCAAACTC10.7407407407407408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT10.7407407407407408No Hit
TCTCCGAGCCCACGAGACGCTACGCTCTCTCGTATGCCGGCTTCTGCTTG10.7407407407407408TruSeq Adapter, Index 16 (95% over 24bp)
GGAGAAACAAGCTTGACCACTATGCTATCATCAAATTCCCACTGACCACG10.7407407407407408No Hit
AACAAAATGCTCCACATGCCATTGCTTGCCCATGGCATTCACTACTCGCC10.7407407407407408No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers