FastQCFastQC Report
Fri 10 Feb 2017
SRR2568598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011917
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.048.3792951
GTATCAA16150.037.953461
TCAACGC17150.034.0763974
ATCAACG17300.034.0572663
CAACGCA17200.033.977345
AACGCAG18550.031.7575846
ACATGGG24650.029.6850663
TACATGG25900.029.5365872
GTACATG26750.029.308551
ATTCCCG650.005835915529.2387875
TATCAAC20600.028.6014922
CATGGGG16500.027.3559864
ACGCAGA21300.027.2113387
CGCAGAG22050.025.8535888
TGTACCG1151.2792188E-424.7894045
GTACCGT1100.00287420421.5946926
GTATTAT6750.021.1179471
TAATACG1150.003717059520.6578374
GCAGAGT28450.019.8706869
GGTAGCG1200.00475828119.7951357
Produced by FastQC (version 0.11.5)