##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568598_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1011917 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.203042344382 34.0 31.0 34.0 30.0 34.0 2 32.32575695437472 34.0 31.0 34.0 31.0 34.0 3 32.456574007552 34.0 31.0 34.0 31.0 34.0 4 35.64150617096066 37.0 35.0 37.0 35.0 37.0 5 35.69166739959898 37.0 37.0 37.0 35.0 37.0 6 35.80901200394894 37.0 37.0 37.0 35.0 37.0 7 35.83997106482053 37.0 37.0 37.0 35.0 37.0 8 35.87180470335018 37.0 37.0 37.0 35.0 37.0 9 37.67379933334453 39.0 39.0 39.0 35.0 39.0 10-11 37.68474934209031 39.0 39.0 39.0 35.0 39.0 12-13 37.669755029315645 39.0 39.0 39.0 35.0 39.0 14-15 39.17610387017908 41.0 40.0 41.0 36.0 41.0 16-17 39.16742578689754 41.0 40.0 41.0 36.0 41.0 18-19 39.190025466515536 41.0 40.0 41.0 36.5 41.0 20-21 39.190738963768766 41.0 40.0 41.0 36.5 41.0 22-23 39.11826365205842 41.0 40.0 41.0 36.0 41.0 24-25 39.05429546099137 41.0 40.0 41.0 36.0 41.0 26-27 38.9810651466474 41.0 39.0 41.0 36.0 41.0 28-29 38.90115444250863 41.0 39.0 41.0 36.0 41.0 30-31 38.79165781383256 41.0 39.0 41.0 35.0 41.0 32-33 38.67985318954025 41.0 39.0 41.0 35.0 41.0 34-35 38.58538200267414 41.0 39.0 41.0 35.0 41.0 36-37 38.47206292610955 40.0 38.5 41.0 34.5 41.0 38-39 38.36271551915819 40.0 38.0 41.0 34.0 41.0 40-41 38.24453784253056 40.0 38.0 41.0 34.0 41.0 42-43 38.14174976801457 40.0 38.0 41.0 33.0 41.0 44-45 38.01202173696064 40.0 38.0 41.0 33.0 41.0 46-47 37.97612007704189 40.0 38.0 41.0 33.0 41.0 48-49 38.09918105931613 40.0 38.0 41.0 33.0 41.0 50-51 36.676386996166684 38.5 36.5 40.0 31.5 40.5 52-53 36.8907494389362 39.5 37.5 39.5 31.5 40.5 54-55 37.72509059537492 40.0 38.0 41.0 33.0 41.0 56-57 37.73750317466749 40.0 37.5 41.0 33.0 41.0 58-59 37.57376642550723 40.0 37.0 41.0 32.5 41.0 60-61 37.37252561227848 40.0 37.0 41.0 32.0 41.0 62-63 37.12760335086771 40.0 36.0 41.0 31.5 41.0 64-65 36.87212785238315 39.0 36.0 41.0 31.0 41.0 66-67 36.80547317615971 39.0 35.0 41.0 31.5 41.0 68-69 36.52453511503414 39.0 35.0 41.0 31.0 41.0 70-71 36.1851357374172 37.5 35.0 40.5 31.0 41.0 72-73 35.84208536866166 37.0 35.0 39.5 31.0 41.0 74-75 35.455978602988196 37.0 35.0 39.0 30.5 41.0 76-77 35.079061820287635 36.0 35.0 39.0 30.0 41.0 78-79 34.71480813149695 36.0 35.0 38.0 30.0 39.5 80-81 34.36516087781903 35.0 35.0 37.0 30.0 39.0 82-83 34.02904783692733 35.0 34.5 37.0 29.0 39.0 84-85 33.71941374638434 35.0 34.0 36.0 29.0 38.0 86-87 33.48626122498189 35.0 34.0 36.0 29.0 37.0 88-89 33.30097231294661 35.0 34.0 36.0 29.0 37.0 90-91 33.09858664297566 35.0 34.0 35.0 29.0 36.0 92-93 32.966275890216295 35.0 34.0 35.0 29.0 36.0 94-95 32.843032580735375 35.0 34.0 35.0 28.0 36.0 96-97 32.727203416881025 35.0 34.0 35.0 27.0 36.0 98-99 32.655506825164515 35.0 34.0 35.0 27.0 36.0 100-101 31.484631150578558 34.5 31.5 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27112690388505456 1101 2 0.23649121999343947 1101 3 0.2860267607352185 1101 4 0.05069739103186066 1101 5 0.09268159659403352 1101 6 0.16720076490029356 1101 7 0.21845875339889176 1101 8 0.296108098680989 1101 9 0.3244845436870989 1101 10-11 0.26778121306480784 1101 12-13 0.39952342475608305 1101 14-15 0.5316243490628736 1101 16-17 0.5412123519576326 1101 18-19 0.47687442644500067 1101 20-21 0.4071707936899074 1101 22-23 0.41500967982324966 1101 24-25 0.3951911105600061 1101 26-27 0.31589515759883113 1101 28-29 0.40025598285005515 1101 30-31 0.390667562237347 1101 32-33 0.37688911073518483 1101 34-35 0.2833630189670444 1101 36-37 0.21925769295780384 1101 38-39 0.24429405231043688 1101 40-41 0.2512498150747362 1101 42-43 0.10198288004392708 1101 44-45 0.32095323450359814 1101 46-47 0.1645311364477422 1101 48-49 0.18694368587983234 1101 50-51 0.17573074263024324 1101 52-53 0.2739821201853019 1101 54-55 0.29147060571805383 1101 56-57 0.3139901847604065 1101 58-59 0.29362152902094607 1101 60-61 0.3185192374238781 1101 62-63 0.23963555635207 1101 64-65 0.4104955737067968 1101 66-67 0.49224429115587043 1101 68-69 0.24796319445641046 1101 70-71 0.24926672924874538 1101 72-73 0.2119710597543687 1101 74-75 0.20081619684088992 1101 76-77 0.24473139161435853 1101 78-79 0.22106869122268336 1101 80-81 0.2530960573816614 1101 82-83 0.33987687889941753 1101 84-85 0.18803448714734827 1101 86-87 0.10441009812154078 1101 88-89 0.21411760288485482 1101 90-91 0.14529244768819183 1101 92-93 0.10152521123756486 1101 94-95 0.0726774220517612 1101 96-97 0.13034192793975308 1101 98-99 -0.02115156416789432 1101 100-101 -0.14811410360390198 1102 1 0.008673398856601011 1102 2 0.12962798147162857 1102 3 0.20040900672898232 1102 4 0.15409714504404803 1102 5 0.03493997909018276 1102 6 0.033289290730508014 1102 7 0.17067874869355393 1102 8 -0.004196556921193917 1102 9 0.2561901683502086 1102 10-11 0.16919251599610874 1102 12-13 0.315711120005183 1102 14-15 0.4417747709051625 1102 16-17 0.47696143843753447 1102 18-19 0.2520047910489609 1102 20-21 0.21728071390766246 1102 22-23 0.3763894450925207 1102 24-25 0.36536732240733016 1102 26-27 0.34574202572137125 1102 28-29 0.29001413454651015 1102 30-31 0.21512354501224706 1102 32-33 0.14693145875730096 1102 34-35 0.18508478471875378 1102 36-37 0.14417185036021607 1102 38-39 0.2705858605590663 1102 40-41 0.1506241245369253 1102 42-43 0.25201087381383047 1102 44-45 0.10892574104789077 1102 46-47 0.2333712413946003 1102 48-49 0.040210171678950246 1102 50-51 0.0823491845684785 1102 52-53 0.16176656220097385 1102 54-55 0.18284733336547276 1102 56-57 0.2552166692491866 1102 58-59 0.2640277960517281 1102 60-61 0.31031765845613535 1102 62-63 0.24297768489111604 1102 64-65 0.24608950322801348 1102 66-67 0.3454772737729286 1102 68-69 0.36982915790370186 1102 70-71 0.42721031882434346 1102 72-73 0.455857101286405 1102 74-75 0.507024113121652 1102 76-77 0.4060313448588033 1102 78-79 0.33682367426263227 1102 80-81 0.34690124456766114 1102 82-83 0.48699307389091473 1102 84-85 0.3947089166253832 1102 86-87 0.4828210943126052 1102 88-89 0.3735004296004405 1102 90-91 0.2782479643965985 1102 92-93 0.2442703577432539 1102 94-95 0.3402911616322726 1102 96-97 0.389547857854069 1102 98-99 0.277253088754037 1102 100-101 0.14537302670709806 1103 1 0.13214337749121086 1103 2 0.29057011236243824 1103 3 0.22805707400285513 1103 4 0.23183834715377571 1103 5 0.06473029021473309 1103 6 0.04390352424363897 1103 7 0.16725429630642452 1103 8 0.2977457251532769 1103 9 0.38088245256406594 1103 10-11 0.3575987265065095 1103 12-13 0.3858011455923034 1103 14-15 0.4388865926111336 1103 16-17 0.5095964268990869 1103 18-19 0.48107156002607354 1103 20-21 0.4975723557336238 1103 22-23 0.5508568079646636 1103 24-25 0.4884885802553285 1103 26-27 0.609866287274798 1103 28-29 0.494398032631608 1103 30-31 0.5341574244133298 1103 32-33 0.5495556361613367 1103 34-35 0.557675473517186 1103 36-37 0.44672171957208917 1103 38-39 0.5798056388040251 1103 40-41 0.5207295537416172 1103 42-43 0.5171770189444302 1103 44-45 0.6407484739348988 1103 46-47 0.6782462581531661 1103 48-49 0.6604582269722385 1103 50-51 0.5299519252713978 1103 52-53 0.6246288681225209 1103 54-55 0.7116620820440431 1103 56-57 0.657038574904881 1103 58-59 0.673530829708632 1103 60-61 0.6267136683486783 1103 62-63 0.6552405333623099 1103 64-65 0.5197126622817763 1103 66-67 0.6094004325414346 1103 68-69 0.5308946603611489 1103 70-71 0.48240518884918515 1103 72-73 0.38675476136278775 1103 74-75 0.4762967485950469 1103 76-77 0.5791894002376594 1103 78-79 0.62202664544931 1103 80-81 0.47226315826746657 1103 82-83 0.3573110159676034 1103 84-85 0.4698336431398573 1103 86-87 0.39668777315002757 1103 88-89 0.4690820160018987 1103 90-91 0.5028776357575708 1103 92-93 0.5017888920034679 1103 94-95 0.3789192983365268 1103 96-97 0.32612274938692565 1103 98-99 0.463488530196031 1103 100-101 0.4015758353000365 1104 1 0.3119841026998387 1104 2 0.3119983812173217 1104 3 0.3359215694007105 1104 4 0.28703989148989706 1104 5 0.3837488621879359 1104 6 0.3637632510801154 1104 7 0.3739302822427959 1104 8 0.3220363411328009 1104 9 0.46921581615476526 1104 10-11 0.5147312961438502 1104 12-13 0.45319943544579644 1104 14-15 0.5577768733390087 1104 16-17 0.7072330237922557 1104 18-19 0.6151994518741333 1104 20-21 0.5206889857888726 1104 22-23 0.5436131937824271 1104 24-25 0.5350940060067657 1104 26-27 0.483988067134284 1104 28-29 0.4432691301874172 1104 30-31 0.6207933495929083 1104 32-33 0.6131036757863839 1104 34-35 0.519669192087342 1104 36-37 0.5383364948506113 1104 38-39 0.5925863515466148 1104 40-41 0.6868667164475895 1104 42-43 0.6002123171913141 1104 44-45 0.7644421120204541 1104 46-47 0.5716026476018143 1104 48-49 0.6006394080988997 1104 50-51 0.505323939625498 1104 52-53 0.5882262272593337 1104 54-55 0.6592169840404267 1104 56-57 0.6514727318438034 1104 58-59 0.5727102498564136 1104 60-61 0.5307388061710014 1104 62-63 0.6833345006480442 1104 64-65 0.7823753712845516 1104 66-67 0.8250658828618569 1104 68-69 0.7019743643230427 1104 70-71 0.6680719704938554 1104 72-73 0.8759937280131211 1104 74-75 0.7762017404558108 1104 76-77 0.7269452111654076 1104 78-79 0.8444394890973825 1104 80-81 0.7784541185758513 1104 82-83 0.7130786756811318 1104 84-85 0.718220925362445 1104 86-87 0.714565642305061 1104 88-89 0.6206442039727733 1104 90-91 0.8298811274579734 1104 92-93 0.7158552403626146 1104 94-95 0.6623506655460076 1104 96-97 0.7911709493760384 1104 98-99 0.6538830257754356 1104 100-101 0.4595822377593528 1105 1 0.22780798140681924 1105 2 0.2752494036859616 1105 3 0.44801664615003034 1105 4 0.22193559113224381 1105 5 0.2498867542768508 1105 6 0.18234565606325503 1105 7 0.2629731831446236 1105 8 0.29485322084941856 1105 9 0.44805627682180926 1105 10-11 0.3993720203425539 1105 12-13 0.4397105499794023 1105 14-15 0.5321937667455856 1105 16-17 0.5290710716044345 1105 18-19 0.541855508824348 1105 20-21 0.5283339709866084 1105 22-23 0.4585215566347287 1105 24-25 0.48341441944345576 1105 26-27 0.40341411569940533 1105 28-29 0.3979553734921737 1105 30-31 0.47650120591681144 1105 32-33 0.4572862998088354 1105 34-35 0.6312637297946182 1105 36-37 0.5752908217325512 1105 38-39 0.4361743180746629 1105 40-41 0.5124695023537811 1105 42-43 0.4907696695536927 1105 44-45 0.37391042832690147 1105 46-47 0.5661352211425523 1105 48-49 0.5154194276070854 1105 50-51 0.38741918749928317 1105 52-53 0.5494237562221542 1105 54-55 0.6416125553394352 1105 56-57 0.6738985921720158 1105 58-59 0.6832138691387186 1105 60-61 0.7511727777985655 1105 62-63 0.5836245566057414 1105 64-65 0.687901835682105 1105 66-67 0.700624176408752 1105 68-69 0.6742222552140902 1105 70-71 0.7389902243398865 1105 72-73 0.6668197457758467 1105 74-75 0.5041309633796445 1105 76-77 0.4737965211231412 1105 78-79 0.5422850321608479 1105 80-81 0.5116454186083672 1105 82-83 0.48257402965207774 1105 84-85 0.6447358046341378 1105 86-87 0.6275659674415337 1105 88-89 0.48585802398407196 1105 90-91 0.5887325400903833 1105 92-93 0.685981187964309 1105 94-95 0.73513417599316 1105 96-97 0.4852389200110778 1105 98-99 0.4590321607412946 1105 100-101 0.3218484389674927 1106 1 0.15132250290706395 1106 2 -0.03490242303834634 1106 3 0.11732260425600316 1106 4 0.10815460374579544 1106 5 0.20026105869671795 1106 6 0.19654061820102697 1106 7 0.20623381452358558 1106 8 0.19586359990348257 1106 9 0.2731398004108172 1106 10-11 0.3350183768789847 1106 12-13 0.4160270922377478 1106 14-15 0.4209886522413555 1106 16-17 0.3602832566031111 1106 18-19 0.44936831397556176 1106 20-21 0.39983583036472936 1106 22-23 0.3832248387474593 1106 24-25 0.46120578048665806 1106 26-27 0.4350883116330593 1106 28-29 0.38008483370579427 1106 30-31 0.3772201294745159 1106 32-33 0.250972240319534 1106 34-35 0.19412096517731214 1106 36-37 0.237595101270756 1106 38-39 0.3873258371515078 1106 40-41 0.2610297661749783 1106 42-43 0.2969706551050564 1106 44-45 0.3117845303471185 1106 46-47 0.20365094084872482 1106 48-49 0.3354766932147939 1106 50-51 0.3242637499652048 1106 52-53 0.4188476458576744 1106 54-55 0.32447794068137625 1106 56-57 0.4521319940513635 1106 58-59 0.5106141940576236 1106 60-61 0.30018182911091884 1106 62-63 0.3325450918043913 1106 64-65 0.2906911727288062 1106 66-67 0.19823451120475966 1106 68-69 0.23512700863734182 1106 70-71 0.23581929648591426 1106 72-73 0.16612753897197052 1106 74-75 0.20815116016607504 1106 76-77 0.1016996067732805 1106 78-79 0.14282908242073233 1106 80-81 0.12351170530342159 1106 82-83 0.12471795469404157 1106 84-85 0.12140857027693386 1106 86-87 0.24560814213132431 1106 88-89 0.13893422880172324 1106 90-91 0.24675944035323027 1106 92-93 0.28551054131092 1106 94-95 0.19370431691728385 1106 96-97 0.09611209908889151 1106 98-99 0.058310538521595845 1106 100-101 0.2009079012860724 1107 1 0.30726710934250434 1107 2 0.32926465030883634 1107 3 0.31534822144988084 1107 4 0.33673030640389356 1107 5 0.3378670306467981 1107 6 0.2479442177527389 1107 7 0.2580101704863438 1107 8 0.2762244869656456 1107 9 0.33034442160937516 1107 10-11 0.27580651145385815 1107 12-13 0.29873021382745435 1107 14-15 0.3989917323864347 1107 16-17 0.3268191724190146 1107 18-19 0.34911891050843025 1107 20-21 0.2834475457063448 1107 22-23 0.2692085846060621 1107 24-25 0.3178343458894659 1107 26-27 0.29341635198884575 1107 28-29 0.21602603138195775 1107 30-31 0.4286905554222855 1107 32-33 0.3316378312242776 1107 34-35 0.28808684815632546 1107 36-37 0.36819104558291116 1107 38-39 0.26863970540664184 1107 40-41 0.4029212106796365 1107 42-43 0.3819775094087987 1107 44-45 0.34942250539696573 1107 46-47 0.3307541330659589 1107 48-49 0.5011160317683547 1107 50-51 0.5166667535971285 1107 52-53 0.5101619310114174 1107 54-55 0.5384891675159196 1107 56-57 0.616298086460624 1107 58-59 0.537498024031656 1107 60-61 0.46469247596591146 1107 62-63 0.6459063246471715 1107 64-65 0.5225143930008684 1107 66-67 0.6061747132289241 1107 68-69 0.5688592567263555 1107 70-71 0.5019955759355099 1107 72-73 0.4205129445775455 1107 74-75 0.4395354107172409 1107 76-77 0.42827529962199407 1107 78-79 0.3911578094330679 1107 80-81 0.40355292921967134 1107 82-83 0.41244421415702703 1107 84-85 0.49166788121709004 1107 86-87 0.38990043220447745 1107 88-89 0.41296107707269414 1107 90-91 0.5456634874578725 1107 92-93 0.49443192649335543 1107 94-95 0.4047561766529739 1107 96-97 0.532931113589612 1107 98-99 0.49347544644830776 1107 100-101 0.5389722164810493 1108 1 0.08699166840507644 1108 2 0.14874210168197521 1108 3 0.11656267562321432 1108 4 0.17859423653261786 1108 5 0.12155643720481635 1108 6 0.16273664307676228 1108 7 0.18370517341303838 1108 8 0.07169297451036272 1108 9 0.1310869896538449 1108 10-11 0.1812350091591881 1108 12-13 0.23366012869718844 1108 14-15 0.2505976010434594 1108 16-17 0.21713827788355644 1108 18-19 0.31793125344314177 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47.0 5 37.0 6 144.0 7 944.0 8 1023.0 9 502.0 10 393.0 11 645.0 12 1091.0 13 1457.0 14 1700.0 15 1764.0 16 1982.0 17 2383.0 18 2763.0 19 3204.0 20 3744.0 21 4444.0 22 5093.0 23 5180.0 24 5055.0 25 5070.0 26 5432.0 27 5788.0 28 6574.0 29 7577.0 30 8963.0 31 11074.0 32 14133.0 33 18742.0 34 28045.0 35 45006.0 36 89327.0 37 212089.0 38 367520.0 39 141241.0 40 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48157623051361 20.25049192802097 12.906843793780604 24.361088047684817 2 14.445079666412688 20.96651939865507 41.40988891982431 23.178512015107934 3 16.166085178949913 27.69232446122793 31.007844881999773 25.133745477822377 4 10.833638021971272 18.18629505552269 41.32866045002759 29.651406472478442 5 11.856461738454954 39.21917357599201 37.09435786870433 11.830006816848714 6 28.600255979458243 40.076437209744654 19.728249671109985 11.59505713968712 7 25.750130767645707 32.926698790597406 25.30928529537637 16.01388514638051 8 24.326308374966935 37.11101930749028 21.92954039078622 16.633131926756562 9 25.79794479496987 15.153595519086382 21.50505677917493 37.543402906768826 10-11 23.337870421552953 26.98883955548423 30.76200297585437 18.91128704710845 12-13 24.303284190107654 24.52514943334953 30.544340108173547 20.627226268369267 14-15 21.831421268501455 26.066000911450594 27.302849273811646 24.799728546236306 16-17 21.880866661913927 28.579752936796492 28.094726044216312 21.444654357073265 18-19 21.47278958543418 28.168624323458662 29.298020759231235 21.060565331875917 20-21 22.355015538203986 27.568060699481535 28.95927198638539 21.1176517759291 22-23 21.971672549445795 27.52199915137466 28.520594513047985 21.98573378613156 24-25 21.905920555201526 27.49913225004469 28.745028077467172 21.84991911728662 26-27 21.907661623353857 27.770983158807905 28.528668450542988 21.79268676729525 28-29 21.89170212787028 27.867604024207036 28.428067645815112 21.81262620210757 30-31 22.088899672209074 27.582863763752858 28.560245199496926 21.767991364541142 32-33 21.852114269530684 27.677836252358663 28.50549170883917 21.964557769271483 34-35 22.071529525917015 27.838693468580523 28.509038343964495 21.580738661537964 36-37 22.234479714016054 27.542534568848914 28.414037286201214 21.808948430933818 38-39 22.023719649328992 27.78358357099682 28.420064623346487 21.772632156327706 40-41 22.171707172202147 27.67925555610553 28.329406523783597 21.81963074790873 42-43 22.022941357842917 27.598769241733535 28.3151113339335 22.063178066490046 44-45 22.112672919950217 27.79570517689732 28.261262219758958 21.8303596833935 46-47 22.23028347454272 27.792877504157097 28.028100007918283 21.9487390133819 48-49 22.05083003518302 27.777808022163963 28.16645938264169 22.004902560011324 50-51 22.17866981035894 27.89715097938486 27.85661754152574 22.067561668730455 52-53 22.188954450981953 27.883039557849376 27.930903570595955 21.997102420572716 54-55 22.125736779550014 27.89596996993727 27.994628012646842 21.983665237865875 56-57 22.205452124368232 27.88726468098588 27.845782202135506 22.061500992510382 58-59 22.342001324803633 27.67488051518821 27.90993909665819 22.073179063349972 60-61 22.26666521420939 27.92020661864975 27.922880460455364 21.890247706685496 62-63 22.140189067361746 27.808199849993688 28.081033112779497 21.97057796986507 64-65 21.97061317679371 27.87926462150641 28.07817344473151 22.071948756968368 66-67 22.01457614115842 28.012918074167565 27.86136580174963 22.111139982924385 68-69 22.16001682564426 27.9435846045355 27.94724665652179 21.949151913298444 70-71 22.172431013686644 27.618282579461773 28.002803353927884 22.206483052923698 72-73 22.083543273804278 27.74624408660799 27.89467901133134 22.275533628256387 74-75 22.03806958295782 27.887702653184515 27.930832994959047 22.143394768898617 76-77 22.13170316505305 27.83475037222957 27.961707022277327 22.07183944044005 78-79 22.09575512298228 27.854820194192452 27.80995169455348 22.239472988271793 80-81 22.09957637085253 27.916739912501143 27.70336551198219 22.28031820466413 82-83 22.195064583297228 27.841157522833793 27.80193960341374 22.16183829045524 84-85 22.199540534920338 27.815807975283946 27.768920752176022 22.215730737619694 86-87 22.0017306228163 27.876591148280124 27.900996098178194 22.220682130725386 88-89 22.072459356384307 27.896777961743403 27.87777093062681 22.15299175124548 90-91 22.100742979847542 27.881762623555723 27.897698113525028 22.11979628307171 92-93 22.173095167748766 27.82287139879967 27.868402181320402 22.135631252131162 94-95 21.93422360884759 27.810774468430488 28.057833099818712 22.19716882290321 96-97 22.02511683023642 27.740084217758394 28.08331093499333 22.151488017011847 98-99 22.058090563389275 27.644121427620412 27.90699055680641 22.390797452183904 100-101 22.142245013443862 27.52721571221275 27.950408959735746 22.380130314607644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1441.0 1 748.0 2 51.0 3 50.0 4 57.5 5 56.5 6 58.5 7 68.5 8 109.0 9 142.0 10 145.5 11 156.5 12 181.0 13 219.0 14 254.0 15 282.5 16 310.5 17 372.5 18 436.0 19 474.5 20 544.0 21 687.0 22 887.0 23 1205.0 24 1776.0 25 2639.5 26 4004.5 27 5902.0 28 8188.0 29 10788.0 30 13868.5 31 17261.5 32 21006.0 33 24635.5 34 27991.5 35 30803.5 36 33419.0 37 35465.0 38 36645.0 39 37852.0 40 38743.0 41 39550.5 42 39615.0 43 39037.0 44 38935.0 45 39290.0 46 38306.0 47 36750.5 48 36164.0 49 35534.5 50 34271.5 51 32450.5 52 30824.5 53 29232.0 54 27153.0 55 24919.0 56 22344.5 57 19783.5 58 17667.5 59 15384.5 60 12608.0 61 9942.5 62 7963.5 63 6132.5 64 4452.5 65 3273.5 66 2470.0 67 1943.0 68 1547.5 69 1212.0 70 946.0 71 739.5 72 572.0 73 451.0 74 342.5 75 257.0 76 194.0 77 125.5 78 80.5 79 56.5 80 44.0 81 36.5 82 26.0 83 19.5 84 11.5 85 8.0 86 7.0 87 7.5 88 5.0 89 4.0 90 4.0 91 3.0 92 3.0 93 1.5 94 1.5 95 2.5 96 1.0 97 0.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25921098271893844 2 0.2614838964065235 3 0.26919203847746404 4 0.26583207911320794 5 0.26217565274622323 6 0.24320176457160023 7 0.24636407926737075 8 0.2486369929549558 9 0.24507938892221395 10-11 0.24300411990311457 12-13 0.24908169345904851 14-15 0.2507122619740552 16-17 0.19497646546110006 18-19 0.18860242490243764 20-21 0.18044958232740432 22-23 0.2024375516964336 24-25 0.20965158209616006 26-27 0.21059039427146695 28-29 0.2102939272687384 30-31 0.21019510493449559 32-33 0.2052045770552328 34-35 0.18919535890789463 36-37 0.16310626266778797 38-39 0.1888000695709233 40-41 0.17461906460707746 42-43 0.16261215099657383 44-45 0.14966642521076334 46-47 0.15781926778579666 48-49 0.16093217131444576 50-51 0.16206862815823828 52-53 0.17427318643722756 54-55 0.18405659752726755 56-57 0.18301896301771786 58-59 0.193296485778972 60-61 0.21088686127419543 62-63 0.1936423639488219 64-65 0.188404780233952 66-67 0.1684426687169007 68-69 0.15361931858047645 70-71 0.1678497347114437 72-73 0.16898619155523625 74-75 0.2161244449890653 76-77 0.2098492267646457 78-79 0.2272913687585049 80-81 0.21637150082467238 82-83 0.21488916581103 84-85 0.20228931819506937 86-87 0.18539069903954572 88-89 0.17437200877147038 90-91 0.15747338961594676 92-93 0.15925219163231769 94-95 0.1611298159829314 96-97 0.1776331458014837 98-99 0.1698261813963003 100-101 0.19532234363095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1011917.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.60756954268301 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70794664043888 48.266988579632056 2 15.364222391161938 19.852901332124844 3 4.933235590828245 9.56173084514624 4 2.0941878484130876 5.4120154820716095 5 1.0299957179753845 3.3272759988880187 6 0.5461976132509417 2.1173100169294616 7 0.3390026201943645 1.533149475155141 8 0.2095759966006596 1.0832156619883369 9 0.15579668104194772 0.9059080414443157 >10 0.5917546071733508 6.150462817791446 >50 0.019955181805742277 0.8819290362804788 >100 0.007822352665310048 0.7440030182287195 >500 3.067584501409167E-4 0.163109694319345 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.882233424282822E-5 7 0.0 0.0 0.0 0.0 9.882233424282822E-5 8 0.0 0.0 0.0 0.0 9.882233424282822E-5 9 0.0 0.0 0.0 4.941116712141411E-4 9.882233424282822E-5 10-11 0.0 0.0 0.0 4.941116712141411E-4 9.882233424282822E-5 12-13 0.0 0.0 0.0 4.941116712141411E-4 2.9646700272848467E-4 14-15 0.0 0.0 0.0 5.435228383355552E-4 2.9646700272848467E-4 16-17 0.0 0.0 0.0 7.905786739426258E-4 2.9646700272848467E-4 18-19 0.0 0.0 0.0 7.905786739426258E-4 3.952893369713129E-4 20-21 0.0 0.0 0.0 8.894010081854539E-4 3.952893369713129E-4 22-23 0.0 0.0 0.0 0.0010870456766711104 3.952893369713129E-4 24-25 0.0 0.0 0.0 0.001877624350613736 3.952893369713129E-4 26-27 0.0 0.0 0.0 0.0021246801862208065 4.44700504092727E-4 28-29 0.0 0.0 0.0 0.002174091353342221 4.941116712141411E-4 30-31 0.0 0.0 0.0 0.0022729136875850493 4.941116712141411E-4 32-33 0.0 0.0 0.0 0.0029152588601634322 4.941116712141411E-4 34-35 0.0 0.0 0.0 0.004101126871077371 4.941116712141411E-4 36-37 0.0 0.0 0.0 0.005781106553205451 4.941116712141411E-4 38-39 0.0 0.0 0.0 0.01057398976398262 5.929340054569693E-4 40-41 0.0 0.0 0.0 0.01768919782946625 5.929340054569693E-4 42-43 0.0 0.0 0.0 0.025990273905863823 5.929340054569693E-4 44-45 0.0 0.0 0.0 0.03592191849726806 6.423451725783834E-4 46-47 0.0 0.0 0.0 0.04822529911050017 7.411675068212117E-4 48-49 0.0 0.0 0.0 0.06077573555933935 7.905786739426258E-4 50-51 0.0 0.0 0.0 0.08039196890654075 7.905786739426258E-4 52-53 0.0 0.0 0.0 0.09822940023737126 7.905786739426258E-4 54-55 0.0 0.0 0.0 0.11784563358457265 7.905786739426258E-4 56-57 0.0 0.0 0.0 0.1414147603014872 7.905786739426258E-4 58-59 0.0 0.0 0.0 0.17111087174145706 7.905786739426258E-4 60-61 0.0 0.0 0.0 0.20643985623326816 7.905786739426258E-4 62-63 0.0 0.0 0.0 0.2458699675961566 7.905786739426258E-4 64-65 0.0 0.0 0.0 0.28633771346859477 7.905786739426258E-4 66-67 0.0 0.0 0.0 0.32823838318755394 7.905786739426258E-4 68-69 0.0 0.0 0.0 0.38357889036353776 7.905786739426258E-4 70-71 0.0 0.0 0.0 0.45339686950609587 7.905786739426258E-4 72-73 0.0 0.0 0.0 0.5313676912236873 8.894010081854539E-4 74-75 0.0 0.0 0.0 0.6089432236043075 8.894010081854539E-4 76-77 0.0 0.0 0.0 0.6899775376834266 8.894010081854539E-4 78-79 0.0 0.0 0.0 0.7961621358273455 8.894010081854539E-4 80-81 0.0 0.0 0.0 0.9159348049296534 8.894010081854539E-4 82-83 0.0 0.0 0.0 1.0528037378559705 0.0010376345095496962 84-85 0.0 0.0 0.0 1.1882891581028878 0.0010870456766711104 86-87 0.0 0.0 0.0 1.326591014875726 0.0010870456766711104 88-89 0.0 0.0 0.0 1.4855961506724364 0.0010870456766711104 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 825 0.0 48.379295 1 GTATCAA 1615 0.0 37.95346 1 TCAACGC 1715 0.0 34.076397 4 ATCAACG 1730 0.0 34.057266 3 CAACGCA 1720 0.0 33.97734 5 AACGCAG 1855 0.0 31.757584 6 ACATGGG 2465 0.0 29.685066 3 TACATGG 2590 0.0 29.536587 2 GTACATG 2675 0.0 29.30855 1 ATTCCCG 65 0.0058359155 29.238787 5 TATCAAC 2060 0.0 28.601492 2 CATGGGG 1650 0.0 27.355986 4 ACGCAGA 2130 0.0 27.211338 7 CGCAGAG 2205 0.0 25.853588 8 TGTACCG 115 1.2792188E-4 24.789404 5 GTACCGT 110 0.002874204 21.594692 6 GTATTAT 675 0.0 21.117947 1 TAATACG 115 0.0037170595 20.657837 4 GCAGAGT 2845 0.0 19.870686 9 GGTAGCG 120 0.004758281 19.795135 7 >>END_MODULE