FastQCFastQC Report
Fri 10 Feb 2017
SRR2568598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011917
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.10554225297134054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.049.6090281
GTATCAA14700.038.466211
TCAACGC16500.033.687274
ATCAACG16600.033.485993
CAACGCA17100.032.2274365
AACGCAG17750.031.573216
GTACATG25200.031.4894941
TACATGG24250.031.1555292
TATCAAC19100.029.35612
ACATGGG24750.029.1778813
CATGGGG15800.027.362024
ACGCAGA20550.027.2712657
CGCAGAG21000.026.6868828
TGTACGA908.928042E-426.3983782
GCAGAGT26900.021.1867379
AGAGTAC23450.021.06326110-11
ATAGACC3201.8189894E-1120.785643
GTATAGG4150.020.609821
GTATAAA9900.018.7188511
GTACCGT1300.007568396618.266776
Produced by FastQC (version 0.11.5)