FastQCFastQC Report
Fri 10 Feb 2017
SRR2568597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659477
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.034.1568071
GTATCAA23250.033.940981
TCAACGC22150.033.6939624
CAACGCA22600.033.0180745
ATCAACG23150.032.239473
AACGCAG23550.032.0897836
ACATGGG38500.028.7696423
ACGCAGA27000.027.6373527
TACATGG43200.026.9601062
GTACATG45150.026.9538921
CGCAGAG27650.026.8141338
TATCAAC29200.025.8853192
TATAGCG950.001222395925.0153685
CATGGGG22550.022.3452634
ACCGTAT1604.9697337E-520.7927688
CCGTATG1604.9708902E-520.792149
GTACCGT1908.211129E-620.0122956
GCAGAGT37450.019.7967519
CATGGGA18400.019.6345924
TACCGTC1759.893363E-519.011687
Produced by FastQC (version 0.11.5)