##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568597_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1659477 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24328508319187 34.0 31.0 34.0 31.0 34.0 2 32.368114170910474 34.0 31.0 34.0 31.0 34.0 3 32.509841353631295 34.0 31.0 34.0 31.0 34.0 4 35.677250121574446 37.0 35.0 37.0 35.0 37.0 5 35.71934410660708 37.0 37.0 37.0 35.0 37.0 6 35.847426026392654 37.0 37.0 37.0 35.0 37.0 7 35.88125114117279 37.0 37.0 37.0 35.0 37.0 8 35.91563727608156 37.0 37.0 37.0 35.0 37.0 9 37.71776891153056 39.0 39.0 39.0 35.0 39.0 10-11 37.73668179793995 39.0 39.0 39.0 36.0 39.0 12-13 37.722028988651246 39.0 39.0 39.0 35.0 39.0 14-15 39.25213516065604 41.0 40.0 41.0 37.0 41.0 16-17 39.25507042278983 41.0 40.0 41.0 37.0 41.0 18-19 39.27552174570663 41.0 40.0 41.0 37.0 41.0 20-21 39.276172251860075 41.0 40.0 41.0 37.0 41.0 22-23 39.21583607365453 41.0 40.0 41.0 37.0 41.0 24-25 39.16636235392235 41.0 40.0 41.0 36.5 41.0 26-27 39.10383904085444 41.0 40.0 41.0 36.0 41.0 28-29 39.03302064445606 41.0 40.0 41.0 36.0 41.0 30-31 38.950243962405025 41.0 39.0 41.0 36.0 41.0 32-33 38.85447463267042 41.0 39.0 41.0 35.0 41.0 34-35 38.783645992080636 41.0 39.0 41.0 35.0 41.0 36-37 38.69610636363143 41.0 39.0 41.0 35.0 41.0 38-39 38.597408099057716 40.0 39.0 41.0 35.0 41.0 40-41 38.502768342074035 40.0 39.0 41.0 35.0 41.0 42-43 38.41652731553375 40.0 38.0 41.0 34.0 41.0 44-45 38.308411023473056 40.0 38.0 41.0 34.0 41.0 46-47 38.29971039068333 40.0 38.5 41.0 33.5 41.0 48-49 38.44191844780012 40.5 39.0 41.0 34.0 41.0 50-51 37.04093428230701 39.5 37.0 40.0 32.0 40.5 52-53 37.261199763539956 39.5 37.5 39.5 33.0 40.5 54-55 38.123553987189936 40.0 38.0 41.0 34.0 41.0 56-57 38.161816945941396 40.0 38.0 41.0 34.0 41.0 58-59 38.0197291074236 40.0 38.0 41.0 33.5 41.0 60-61 37.845495598914596 40.0 37.5 41.0 33.0 41.0 62-63 37.62339218922588 40.0 37.0 41.0 33.0 41.0 64-65 37.405287629777334 40.0 36.5 41.0 32.5 41.0 66-67 37.37028292648829 39.5 36.0 41.0 33.0 41.0 68-69 37.1281271147476 39.0 36.0 41.0 32.5 41.0 70-71 36.82982017828509 39.0 35.0 41.0 32.0 41.0 72-73 36.52512870018687 38.5 35.0 40.5 32.0 41.0 74-75 36.16247799758599 37.0 35.0 40.0 32.0 41.0 76-77 35.78358030873582 37.0 35.0 39.0 31.0 41.0 78-79 35.391203373110926 36.0 35.0 39.0 31.0 40.5 80-81 35.00974312991383 36.0 35.0 38.0 31.0 39.0 82-83 34.64033517789038 35.5 35.0 37.0 31.0 39.0 84-85 34.295859478618866 35.0 35.0 37.0 30.5 39.0 86-87 34.02073243558061 35.0 35.0 36.5 30.0 37.0 88-89 33.800156916908165 35.0 34.5 36.0 30.0 37.0 90-91 33.564488088717106 35.0 34.0 36.0 30.0 37.0 92-93 33.40553409297026 35.0 34.0 35.5 30.0 36.0 94-95 33.27102454568518 35.0 34.0 35.0 29.0 36.0 96-97 33.144796523241965 35.0 34.0 35.0 29.0 36.0 98-99 33.0661666296068 35.0 34.0 35.0 29.0 36.0 100-101 31.940600864007152 34.5 32.0 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10834811374270714 1101 2 0.17203138953010466 1101 3 0.2203452418456564 1101 4 -0.021265238321134916 1101 5 0.1318140612748948 1101 6 0.02733597448235514 1101 7 0.18001880841864448 1101 8 0.26850789580748113 1101 9 0.3438290211721551 1101 10-11 0.30810100909626925 1101 12-13 0.38017177813455305 1101 14-15 0.45108867801912567 1101 16-17 0.39667354017132084 1101 18-19 0.40896074250334635 1101 20-21 0.46085068541606233 1101 22-23 0.46684042563096995 1101 24-25 0.4272479998860632 1101 26-27 0.39222142386401515 1101 28-29 0.43038996835199583 1101 30-31 0.46626873104479927 1101 32-33 0.4588346406924799 1101 34-35 0.42719489679208067 1101 36-37 0.40515967886274495 1101 38-39 0.36172967346566054 1101 40-41 0.381315804057067 1101 42-43 0.3168275989229201 1101 44-45 0.3601590785298896 1101 46-47 0.4328609016148235 1101 48-49 0.47739839096188064 1101 50-51 0.30560337590338094 1101 52-53 0.4263951734543383 1101 54-55 0.4480917866707941 1101 56-57 0.4417524630754528 1101 58-59 0.4020523625200454 1101 60-61 0.38428720189036625 1101 62-63 0.3824446953130476 1101 64-65 0.40782535705741907 1101 66-67 0.4053337043092071 1101 68-69 0.5035659762955831 1101 70-71 0.405122427515586 1101 72-73 0.4518239142001974 1101 74-75 0.41566998419430945 1101 76-77 0.36131440994767416 1101 78-79 0.3792703022982451 1101 80-81 0.3257106786241053 1101 82-83 0.2646467079057473 1101 84-85 0.2035776489128267 1101 86-87 0.16907967743895824 1101 88-89 0.2425721186769536 1101 90-91 0.21914401436620778 1101 92-93 0.23989853524737725 1101 94-95 0.18151136706633508 1101 96-97 0.2281845956216415 1101 98-99 0.18309943053574784 1101 100-101 0.20634363392769117 1102 1 0.07239598637978872 1102 2 0.23120671659282976 1102 3 0.29776818088075885 1102 4 0.10977210863852349 1102 5 0.09737264125330825 1102 6 0.06361027778365269 1102 7 0.22584901249408773 1102 8 0.07806184728183041 1102 9 0.1524705920478837 1102 10-11 0.30423056605832954 1102 12-13 0.34444896423366345 1102 14-15 0.44879229456586955 1102 16-17 0.4172519190794617 1102 18-19 0.3282372598843324 1102 20-21 0.3331206504423818 1102 22-23 0.4201980133074983 1102 24-25 0.42145289403637065 1102 26-27 0.3393669721235355 1102 28-29 0.4305608028201604 1102 30-31 0.16017397449881088 1102 32-33 0.29839075264077053 1102 34-35 0.31151153455315495 1102 36-37 0.2110825179962319 1102 38-39 0.31590082307063483 1102 40-41 0.27644157991898766 1102 42-43 0.28170527765340836 1102 44-45 0.273851934941753 1102 46-47 0.31173628558627087 1102 48-49 0.4001378935763782 1102 50-51 0.2306679599959054 1102 52-53 0.22527021088359334 1102 54-55 0.3071414380439492 1102 56-57 0.312651420579094 1102 58-59 0.3792821042865526 1102 60-61 0.3733659790512789 1102 62-63 0.28699262832427763 1102 64-65 0.3150652189386989 1102 66-67 0.41216356346688343 1102 68-69 0.41837588978457063 1102 70-71 0.3311640981304862 1102 72-73 0.385403960301133 1102 74-75 0.39341358304574214 1102 76-77 0.2848246981895457 1102 78-79 0.2616969314661546 1102 80-81 0.07878177337794767 1102 82-83 0.17264544302675944 1102 84-85 0.25385144431323425 1102 86-87 0.31345294235908483 1102 88-89 0.2128147036217527 1102 90-91 0.28080388645965115 1102 92-93 0.18362116721361588 1102 94-95 0.26869759071492183 1102 96-97 0.19560421543236828 1102 98-99 0.19038412631231694 1102 100-101 0.08738003331106725 1103 1 0.059217301940940104 1103 2 0.09192223266094857 1103 3 0.1723768979832272 1103 4 0.18295902277744602 1103 5 0.11873105213437896 1103 6 0.10980664650823968 1103 7 0.10799152027720993 1103 8 0.18913409001332582 1103 9 0.3543816049602242 1103 10-11 0.28483282273990795 1103 12-13 0.3793393305935382 1103 14-15 0.42858327719630296 1103 16-17 0.5109434392985861 1103 18-19 0.4743574985668957 1103 20-21 0.4596164037470203 1103 22-23 0.5003612994884037 1103 24-25 0.4590181787372103 1103 26-27 0.48075699398434324 1103 28-29 0.4082850568308132 1103 30-31 0.42948692213494866 1103 32-33 0.38971809480916164 1103 34-35 0.3770732168994968 1103 36-37 0.39382306128403144 1103 38-39 0.496735988770439 1103 40-41 0.446923697865266 1103 42-43 0.378564275458821 1103 44-45 0.5171791261259884 1103 46-47 0.5519364033964465 1103 48-49 0.4356842967508783 1103 50-51 0.32591052349431493 1103 52-53 0.4702521140495861 1103 54-55 0.5742510270267829 1103 56-57 0.5432228424671166 1103 58-59 0.4740602851613218 1103 60-61 0.3861616209253569 1103 62-63 0.37151744289021593 1103 64-65 0.5215118704927661 1103 66-67 0.5001243309767958 1103 68-69 0.5191059832671598 1103 70-71 0.4802030095134171 1103 72-73 0.41914759132136226 1103 74-75 0.41602824594701815 1103 76-77 0.4555851267293818 1103 78-79 0.42639194345982645 1103 80-81 0.4198560575854273 1103 82-83 0.4842305002731493 1103 84-85 0.45487702017958753 1103 86-87 0.34626701068048504 1103 88-89 0.48396657454943437 1103 90-91 0.5195494526289934 1103 92-93 0.4916572992120507 1103 94-95 0.43208318725120876 1103 96-97 0.3541187120384137 1103 98-99 0.40359609106283045 1103 100-101 0.5561991457793845 1104 1 0.279407596772586 1104 2 0.34488088410520135 1104 3 0.3361131327226943 1104 4 0.22359512734065135 1104 5 0.2403787934002537 1104 6 0.2785641228478255 1104 7 0.29987287516586036 1104 8 0.2587146233841864 1104 9 0.3830698591909467 1104 10-11 0.35374378393331796 1104 12-13 0.4227048488358065 1104 14-15 0.5808180430549399 1104 16-17 0.4776924189981173 1104 18-19 0.44714252301893254 1104 20-21 0.4353969116103684 1104 22-23 0.46405322592121934 1104 24-25 0.44342781178087165 1104 26-27 0.4091375088842497 1104 28-29 0.4924043220385741 1104 30-31 0.4896950942139 1104 32-33 0.49443302637865827 1104 34-35 0.42606193202457376 1104 36-37 0.42268997804158914 1104 38-39 0.4901474645119066 1104 40-41 0.4247645969610758 1104 42-43 0.47165239951328175 1104 44-45 0.5793806222734688 1104 46-47 0.4077447496301332 1104 48-49 0.4961596380237836 1104 50-51 0.42221952060932466 1104 52-53 0.5687078101407792 1104 54-55 0.6087950142906138 1104 56-57 0.5431117968350563 1104 58-59 0.506684612290762 1104 60-61 0.5465626385871118 1104 62-63 0.4912053936695102 1104 64-65 0.5133610111534992 1104 66-67 0.5236082832337701 1104 68-69 0.44861564533660925 1104 70-71 0.4825349677866413 1104 72-73 0.39095904243490054 1104 74-75 0.3929681361767621 1104 76-77 0.48918797477497833 1104 78-79 0.40458522003161335 1104 80-81 0.3601047118443006 1104 82-83 0.3880726791423754 1104 84-85 0.44518581499364274 1104 86-87 0.39495007545197325 1104 88-89 0.4514494449360882 1104 90-91 0.4830962501169438 1104 92-93 0.5124497692187333 1104 94-95 0.48231869194810884 1104 96-97 0.48525645699848496 1104 98-99 0.4472689088512425 1104 100-101 0.3411807188012297 1105 1 0.21879739659398467 1105 2 0.22276948709584588 1105 3 0.22634010239618618 1105 4 0.17255345642629294 1105 5 0.14997321661754626 1105 6 0.14606354176305558 1105 7 0.2950977855564716 1105 8 0.2241941100722471 1105 9 0.36930630564347666 1105 10-11 0.33186747139534134 1105 12-13 0.3334104692508433 1105 14-15 0.4868658102621879 1105 16-17 0.45630442155896844 1105 18-19 0.42682137683431165 1105 20-21 0.3791152832681064 1105 22-23 0.4235409130407106 1105 24-25 0.35202419641413485 1105 26-27 0.3758795806451545 1105 28-29 0.476741029061742 1105 30-31 0.45934513845789837 1105 32-33 0.41676238115028497 1105 34-35 0.42927930666317593 1105 36-37 0.35721650609065136 1105 38-39 0.3676389929075867 1105 40-41 0.3664578372121028 1105 42-43 0.3100838172377962 1105 44-45 0.267542498219008 1105 46-47 0.3374773283008565 1105 48-49 0.2578562628387573 1105 50-51 0.20418479008993984 1105 52-53 0.2965210760003174 1105 54-55 0.3375028448666839 1105 56-57 0.3601322795635937 1105 58-59 0.30441155138084497 1105 60-61 0.2594094034740948 1105 62-63 0.31157475969582293 1105 64-65 0.45348685168753633 1105 66-67 0.4498604699475166 1105 68-69 0.4132275826528584 1105 70-71 0.36452531566272484 1105 72-73 0.34841344257191054 1105 74-75 0.38130515311181057 1105 76-77 0.2471309958449055 1105 78-79 0.240513895443776 1105 80-81 0.22240495121045 1105 82-83 0.23675157781342904 1105 84-85 0.22214356095562238 1105 86-87 0.248748127084923 1105 88-89 0.15308739038344044 1105 90-91 0.14750246303455583 1105 92-93 0.16249605211119444 1105 94-95 0.14547761149744076 1105 96-97 0.13765766239258426 1105 98-99 0.1569849520921096 1105 100-101 0.1219058310423371 1106 1 0.06407178384743872 1106 2 0.11519603009502077 1106 3 0.27894488823406505 1106 4 0.2177242643061703 1106 5 0.24008040557535537 1106 6 0.2473369010697013 1106 7 0.3430895619819623 1106 8 0.40184058268452816 1106 9 0.3960260567391032 1106 10-11 0.4777839330611613 1106 12-13 0.4807148552880278 1106 14-15 0.46167817073410333 1106 16-17 0.5965691374807705 1106 18-19 0.4977286183665015 1106 20-21 0.5575591993531219 1106 22-23 0.48328492681990554 1106 24-25 0.45746525229598944 1106 26-27 0.44383637941476195 1106 28-29 0.4643178798564449 1106 30-31 0.4821473136247434 1106 32-33 0.5993906685202717 1106 34-35 0.39957478252015477 1106 36-37 0.534481912541608 1106 38-39 0.38268105241080264 1106 40-41 0.39415011623295726 1106 42-43 0.4245522667251933 1106 44-45 0.39945661757854367 1106 46-47 0.37057564631307827 1106 48-49 0.34242990031879117 1106 50-51 0.2621151839662019 1106 52-53 0.411722231851968 1106 54-55 0.26240583729047984 1106 56-57 0.48027748685965577 1106 58-59 0.4857759938374002 1106 60-61 0.5252135952999595 1106 62-63 0.5604555924112091 1106 64-65 0.5134716678929934 1106 66-67 0.28603046567095447 1106 68-69 0.34363322929809925 1106 70-71 0.31843102312161875 1106 72-73 0.43695811313850186 1106 74-75 0.42348186972414226 1106 76-77 0.3683007169260577 1106 78-79 0.429119085398149 1106 80-81 0.39507058993030597 1106 82-83 0.24903936761007373 1106 84-85 0.38871949758387103 1106 86-87 0.3690541962869318 1106 88-89 0.37343534253290755 1106 90-91 0.2948421602218332 1106 92-93 0.24665723574094045 1106 94-95 0.30361025979445344 1106 96-97 0.3105008377281422 1106 98-99 0.4163389738359271 1106 100-101 0.3364230295341635 1107 1 0.19676987111373023 1107 2 0.14502918719532687 1107 3 0.11019840280490456 1107 4 0.14335392741858044 1107 5 0.14143565099666233 1107 6 0.05073618053222617 1107 7 0.09994986973056541 1107 8 0.05792519606156077 1107 9 0.0875267818505634 1107 10-11 0.16518959458074534 1107 12-13 0.10434770404781091 1107 14-15 0.16066005505528125 1107 16-17 -0.015698026405182475 1107 18-19 0.024836890018271163 1107 20-21 0.08265088088525374 1107 22-23 0.10613616193090536 1107 24-25 0.12399940787074115 1107 26-27 0.19087539552327115 1107 28-29 0.08563951992672258 1107 30-31 0.10623419785175514 1107 32-33 -0.0060794162599222545 1107 34-35 0.07515197931190443 1107 36-37 0.035375857928201526 1107 38-39 -0.025834500490049095 1107 40-41 0.0648203667529188 1107 42-43 0.21904101606263993 1107 44-45 0.05751477178647946 1107 46-47 0.16341237919493068 1107 48-49 0.12067830547717762 1107 50-51 0.008977294403770486 1107 52-53 -0.027050917230411642 1107 54-55 -0.011886741819317592 1107 56-57 0.05316396857582362 1107 58-59 0.03042079684654908 1107 60-61 -0.024707436161001795 1107 62-63 -0.09642812338814366 1107 64-65 0.07224134612646793 1107 66-67 0.11033622862542103 1107 68-69 0.12942400437363943 1107 70-71 0.09106351403418245 1107 72-73 0.0614768355432318 1107 74-75 0.09791489713846602 1107 76-77 0.08833572137897505 1107 78-79 -0.09749344678792227 1107 80-81 4.606299392406754E-4 1107 82-83 0.06192496143464865 1107 84-85 -0.03282855483939784 1107 86-87 -0.12938593964119605 1107 88-89 -0.09632656799452377 1107 90-91 0.018228282745482716 1107 92-93 -0.02462996489833813 1107 94-95 -0.1471265556882173 1107 96-97 -0.10399991159854949 1107 98-99 -0.0733460129936816 1107 100-101 -0.1409115199415787 1108 1 0.26096166040599655 1108 2 0.2718625413591198 1108 3 0.2751142148747263 1108 4 0.3330459217263879 1108 5 0.3460312880029548 1108 6 0.33707929540890547 1108 7 0.33644351205629874 1108 8 0.34328489417344343 1108 9 0.3703846326299498 1108 10-11 0.3875623807966875 1108 12-13 0.46062907306919243 1108 14-15 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-0.7351254409920074 2316 50-51 -0.7728816968822017 2316 52-53 -0.9184201148842703 2316 54-55 -0.6956793817117699 2316 56-57 -0.8321490240384719 2316 58-59 -0.8376185882908018 2316 60-61 -0.725228757107466 2316 62-63 -1.256913355962105 2316 64-65 -0.9379858265776022 2316 66-67 -0.8507235372402633 2316 68-69 -0.6899864988631137 2316 70-71 -0.7144769713584296 2316 72-73 -0.7617025420723778 2316 74-75 -0.8093731936986046 2316 76-77 -0.5410642994258552 2316 78-79 -0.6748601958737126 2316 80-81 -0.5269865378036798 2316 82-83 -0.48361720434780153 2316 84-85 -0.5682815799412211 2316 86-87 -0.43839963081754973 2316 88-89 -0.42523287425757417 2316 90-91 -0.427536334645751 2316 92-93 -0.3829094857471773 2316 94-95 -0.4358035287459643 2316 96-97 -0.5479341043312616 2316 98-99 -0.46634878963786264 2316 100-101 -0.3907258768723878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2665.0 3 154.0 4 82.0 5 54.0 6 219.0 7 1249.0 8 1325.0 9 618.0 10 615.0 11 1093.0 12 1886.0 13 2491.0 14 2803.0 15 2920.0 16 2969.0 17 3127.0 18 3419.0 19 3925.0 20 4544.0 21 5443.0 22 6138.0 23 6456.0 24 6491.0 25 6839.0 26 7444.0 27 8392.0 28 9524.0 29 11020.0 30 13128.0 31 16094.0 32 20410.0 33 27548.0 34 40885.0 35 66502.0 36 130211.0 37 307354.0 38 618319.0 39 315030.0 40 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52979544239246 20.97152023975324 13.493551495889816 23.00513282196449 2 14.01973401973402 20.50126585337853 43.37188294934774 22.107117177539713 3 17.411193678545377 27.268705935246906 31.05373490233616 24.266365483871553 4 11.056840217370944 17.30610375335299 42.18985574067282 29.447200288603252 5 11.477122492731064 39.54197855211034 37.38685718567144 11.59404176948715 6 28.655401062282216 40.59761832267668 19.159924702652805 11.587055912388298 7 25.693915821474057 33.396405605457566 24.44050544813937 16.46917312492901 8 23.804917893139347 38.70619089535476 21.113253154714688 16.37563805679121 9 24.987600502383543 16.757958785942684 21.480823217900706 36.77361749377307 10-11 23.188886891987607 27.518897146663 30.848208150277056 18.44400781107233 12-13 23.721199847998147 24.886799098380116 30.79626375100212 20.595737302619614 14-15 20.896602202326058 27.016842933909373 27.386887843767944 24.699667019996625 16-17 20.9535195810709 30.419668775501883 27.90594323202773 20.720868411399486 18-19 20.77868600058205 29.604534646841675 29.334976023803517 20.281803328772764 20-21 21.5383649408702 28.771924634559237 29.20881742813743 20.480892996433134 22-23 21.189520925901547 28.656552652015172 29.182795023629428 20.971131398453853 24-25 21.028075904195457 28.668892511733397 29.103925746919785 21.19910583715136 26-27 21.064813346945762 28.8884882864553 28.96089868613474 21.0857996804642 28-29 21.14022421234889 28.72661082797201 28.942754769328698 21.190410190350402 30-31 21.337864365559366 28.534572019466854 29.018465244689935 21.109098370283853 32-33 21.145540983458062 28.73780339595125 29.03959527580215 21.077060344788542 34-35 21.176465615851647 28.772951923567504 29.070318224206943 20.980264236373905 36-37 21.382199262598483 28.6158389209889 28.87422523342597 21.127736582986646 38-39 21.205929599664774 28.85555152021239 28.873876607256555 21.064642272866283 40-41 21.28818624306682 28.520333547145604 29.130770101497944 21.060710108289626 42-43 21.26002099421301 28.475921545145106 28.997034371232978 21.26702308940891 44-45 21.25613923907385 28.89645338704932 28.63261133618511 21.214796037691723 46-47 21.389121611670316 28.595607213306323 28.708118953399374 21.307152221623983 48-49 21.27481634845805 28.70879535453651 28.78017348053626 21.236214816469182 50-51 21.309949512387693 28.717938193118137 28.5110440142843 21.46106828020987 52-53 21.391296998382074 28.672997512738164 28.67837048127309 21.257335007606677 54-55 21.16172814722126 28.603242502411273 28.81268744915194 21.42234190121552 56-57 21.40335391430375 28.832547899584824 28.491909846232893 21.272188339878532 58-59 21.402788428444627 28.482033415251216 28.690018295665194 21.42515986063896 60-61 21.514095564788867 28.77699536187071 28.565441105420813 21.143467967919605 62-63 21.375879613024612 28.697967425183833 28.643835070519998 21.282317891271557 64-65 21.283268125392453 28.6704825387625 28.661093802830507 21.385155533014537 66-67 21.247841887307278 28.62496528909655 28.74370676228766 21.38348606130851 68-69 21.379845447845742 28.47254957939675 28.632135573251116 21.515469399506394 70-71 21.227706706460165 28.41057811647325 28.80323104858767 21.558484128478913 72-73 21.208149792367553 28.643105811957863 28.604561454555995 21.54418294111859 74-75 21.261349786623406 28.513677723435432 28.688491917297092 21.53648057264407 76-77 21.11281684595 28.525224476583656 28.852246456808945 21.509712220657402 78-79 21.136418959282278 28.663106158668107 28.601375116349725 21.59909976569989 80-81 21.216697589470783 28.693342416907043 28.49355211217247 21.5964078814497 82-83 21.283001410512195 28.705146180217618 28.62750912484528 21.384343284424908 84-85 21.42798049987047 28.554416726198063 28.594241869964108 21.423360903967357 86-87 20.904623887453777 28.56616289702312 28.888089238561488 21.64112397696162 88-89 21.119483425865475 28.37897759155641 28.738541394058327 21.7629975885198 90-91 21.22727877694639 28.453736060337825 28.657660819737092 21.66132434297869 92-93 21.312178465301265 28.406721059839935 28.757719398105756 21.523381076753047 94-95 21.10987671423471 28.34700375057457 28.79108481755222 21.752034717638505 96-97 21.094612424757507 28.27557082566985 28.915726295879395 21.714090453693245 98-99 21.083133089066013 28.25763793160173 28.744955157458246 21.91427382187401 100-101 21.117112863095343 28.27656459595656 28.825024960894346 21.781297580053746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2494.0 1 1285.0 2 68.0 3 69.5 4 77.0 5 68.0 6 61.0 7 71.5 8 103.5 9 122.0 10 124.5 11 125.5 12 135.0 13 162.0 14 189.0 15 202.5 16 218.0 17 247.5 18 285.0 19 319.5 20 351.0 21 419.5 22 490.0 23 586.5 24 751.5 25 1054.0 26 1712.0 27 2879.0 28 5235.0 29 9970.5 30 18375.0 31 30907.5 32 44600.5 33 58110.0 34 71509.5 35 81855.0 36 92407.0 37 99020.0 38 96233.5 39 89595.5 40 79479.0 41 69212.0 42 61187.5 43 54160.0 44 49032.0 45 45597.0 46 43750.0 47 42841.5 48 42326.0 49 42158.0 50 41416.5 51 40222.0 52 38947.5 53 37973.5 54 36654.0 55 34308.5 56 31526.5 57 28278.0 58 25517.5 59 22292.5 60 18303.5 61 14886.5 62 11969.5 63 9253.0 64 6890.5 65 5025.5 66 3642.0 67 2794.5 68 2199.5 69 1626.0 70 1227.0 71 974.5 72 713.5 73 483.5 74 368.5 75 283.0 76 192.5 77 135.0 78 95.5 79 71.0 80 54.0 81 39.0 82 32.0 83 18.5 84 12.5 85 13.5 86 11.0 87 8.5 88 6.5 89 5.0 90 5.5 91 2.5 92 2.0 93 3.0 94 2.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2676746950997212 2 0.26918119383396094 3 0.2812934436572487 4 0.2777983665938124 5 0.2705069127200919 6 0.2514647687193013 7 0.25508036568147674 8 0.25646634451697736 9 0.25116346897245334 10-11 0.24914476066857208 12-13 0.2551406256308464 14-15 0.25746063368157557 16-17 0.2018407004134435 18-19 0.19590509540053885 20-21 0.18807130198249208 22-23 0.21036748324924057 24-25 0.2185327063888201 26-27 0.21919556583188557 28-29 0.2191051759078312 30-31 0.219135305882516 32-33 0.21298879104681775 34-35 0.19708016441324586 36-37 0.17168059575396344 38-39 0.197501984058833 40-41 0.18316011610887045 42-43 0.17053565671594123 44-45 0.15754963762679447 46-47 0.16637772020943947 48-49 0.16914967788044064 50-51 0.17071643656405 52-53 0.18300946623544648 54-55 0.192500408261157 56-57 0.19123494932439558 58-59 0.20169005054001954 60-61 0.2191654358572008 62-63 0.2019310903374979 64-65 0.19506145610936457 66-67 0.1769834712984874 68-69 0.16209926380419853 70-71 0.17626035190605233 72-73 0.17740529094407456 74-75 0.22410675170550723 76-77 0.2184423164647657 78-79 0.2354958821363598 80-81 0.2254324705916382 82-83 0.22269064289532184 84-85 0.2101867034011318 86-87 0.19268118810926574 88-89 0.1823164768176962 90-91 0.16502187134862367 92-93 0.16716109955124417 94-95 0.16857720836142953 96-97 0.18575129393176285 98-99 0.17827906020993362 100-101 0.20407031854011837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1659477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.38636972694972 #Duplication Level Percentage of deduplicated Percentage of total 1 67.38049508709256 31.25536557494794 2 15.670568313197828 14.538015512148345 3 6.15829189767638 8.56982414556286 4 3.1871498549350683 5.913612461848489 5 1.9009484654544884 4.408904917522481 6 1.2507571023277961 3.481084883231126 7 0.8595601407937606 2.7910312145385836 8 0.6384911394542945 2.3693828849686653 9 0.4849774560536896 2.024670922716776 >10 2.338962487720422 18.67216642231806 >50 0.09417531792119879 2.987597569161414 >100 0.03444845902941904 2.62690139987457 >500 0.0010438029716286464 0.2951091152879164 >1k 1.304753714535808E-4 0.0663329758727556 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.2051989873918107E-4 0.0 7 0.0 0.0 0.0 1.2051989873918107E-4 0.0 8 0.0 0.0 0.0 1.2051989873918107E-4 0.0 9 0.0 0.0 0.0 1.2051989873918107E-4 0.0 10-11 0.0 0.0 0.0 1.2051989873918107E-4 0.0 12-13 0.0 0.0 0.0 1.2051989873918107E-4 0.0 14-15 0.0 0.0 0.0 1.2051989873918107E-4 6.025994936959054E-5 16-17 0.0 6.025994936959054E-5 0.0 1.8077984810877163E-4 6.025994936959054E-5 18-19 0.0 6.025994936959054E-5 0.0 2.109098227935669E-4 6.025994936959054E-5 20-21 0.0 6.025994936959054E-5 0.0 2.4103979747836215E-4 6.025994936959054E-5 22-23 3.012997468479527E-5 6.025994936959054E-5 0.0 2.7116977216315745E-4 6.025994936959054E-5 24-25 6.025994936959054E-5 6.025994936959054E-5 0.0 4.51949620271929E-4 6.025994936959054E-5 26-27 6.025994936959054E-5 6.025994936959054E-5 0.0 8.135093164894722E-4 6.025994936959054E-5 28-29 6.025994936959054E-5 6.025994936959054E-5 0.0 0.0010846790886526298 6.025994936959054E-5 30-31 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.0015064987342397634 6.025994936959054E-5 32-33 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.0026213077975771886 6.025994936959054E-5 34-35 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.004157936506501748 6.025994936959054E-5 36-37 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.006960024152187707 6.025994936959054E-5 38-39 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.0146431676968105 6.025994936959054E-5 40-41 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.026062428102347907 6.025994936959054E-5 42-43 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.03913883711554905 6.025994936959054E-5 44-45 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.05408330455920751 6.025994936959054E-5 46-47 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.07240232916756303 9.03899240543858E-5 48-49 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.09445747063683317 1.2051989873918107E-4 50-51 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.12250847706837756 1.2051989873918107E-4 52-53 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.15363274091777107 1.2051989873918107E-4 54-55 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.18894507124835114 1.2051989873918107E-4 56-57 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.2293192373259768 1.2051989873918107E-4 58-59 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.2782503162140843 1.2051989873918107E-4 60-61 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.3356177880139345 1.2051989873918107E-4 62-63 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.39660085677596013 1.2051989873918107E-4 64-65 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.4620732917660203 1.2051989873918107E-4 66-67 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.5369462788577365 1.2051989873918107E-4 68-69 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.6303190704059171 1.2051989873918107E-4 70-71 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.7414685470181268 2.109098227935669E-4 72-73 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.858885058364774 3.6155969621754326E-4 74-75 1.2051989873918107E-4 6.025994936959054E-5 0.0 0.9863649812561427 3.6155969621754326E-4 76-77 1.2051989873918107E-4 6.025994936959054E-5 0.0 1.1192984295654593 3.6155969621754326E-4 78-79 1.2051989873918107E-4 6.025994936959054E-5 0.0 1.2799815845594726 3.6155969621754326E-4 80-81 1.2051989873918107E-4 6.025994936959054E-5 0.0 1.4635032603645608 3.6155969621754326E-4 82-83 1.2051989873918107E-4 6.025994936959054E-5 0.0 1.6782395899430966 3.6155969621754326E-4 84-85 1.2051989873918107E-4 6.025994936959054E-5 0.0 1.8889385029138699 3.6155969621754326E-4 86-87 1.2051989873918107E-4 6.025994936959054E-5 0.0 2.1054525009988083 3.6155969621754326E-4 88-89 1.2051989873918107E-4 6.025994936959054E-5 0.0 2.3634554742247103 3.6155969621754326E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1350 0.0 34.156807 1 GTATCAA 2325 0.0 33.94098 1 TCAACGC 2215 0.0 33.693962 4 CAACGCA 2260 0.0 33.018074 5 ATCAACG 2315 0.0 32.23947 3 AACGCAG 2355 0.0 32.089783 6 ACATGGG 3850 0.0 28.769642 3 ACGCAGA 2700 0.0 27.637352 7 TACATGG 4320 0.0 26.960106 2 GTACATG 4515 0.0 26.953892 1 CGCAGAG 2765 0.0 26.814133 8 TATCAAC 2920 0.0 25.885319 2 TATAGCG 95 0.0012223959 25.015368 5 CATGGGG 2255 0.0 22.345263 4 ACCGTAT 160 4.9697337E-5 20.792768 8 CCGTATG 160 4.9708902E-5 20.79214 9 GTACCGT 190 8.211129E-6 20.012295 6 GCAGAGT 3745 0.0 19.796751 9 CATGGGA 1840 0.0 19.634592 4 TACCGTC 175 9.893363E-5 19.01168 7 >>END_MODULE