FastQCFastQC Report
Fri 10 Feb 2017
SRR2568597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659477
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.041.361571
ATCAACG22900.032.7770233
TCAACGC22350.032.7284664
GTATCAA25000.032.1198231
CAACGCA23350.031.5302415
AACGCAG23800.030.9303516
ACATGGG43100.027.5556493
TATCAAC28200.026.7876832
TACATGG46500.026.1561722
ACGCAGA28600.025.9053027
CGCAGAG28500.025.8295568
GTACATG48900.025.6520161
CATCGCG651.0035197E-525.57472640-41
TCGCGCG601.7127623E-423.74795742-43
CATGGGG24350.023.017894
ATTACGC1050.002195034222.6218763
CATGGGA19150.021.3310454
TATACTG13050.020.3826185
TACCGTG2108.614534E-720.356625
TGTACCG1656.326132E-520.1509975
Produced by FastQC (version 0.11.5)