FastQCFastQC Report
Fri 10 Feb 2017
SRR2568596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311140
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC34290.10355949914530947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.046.1959461
GTATCAA48700.037.667431
TCAACGC49850.035.557534
CAACGCA50800.034.7028585
ATCAACG51950.033.8472823
AACGCAG53050.033.588286
GTACATG100350.030.3089071
ACATGGG96650.029.1581843
CGCAGAG61500.028.3556378
TATCAAC63750.027.8804042
ACGCAGA63500.027.8366977
TACATGG108100.027.1248362
CATGGGG61600.026.2292654
GTATAGT9900.021.6015511
GCAGAGT81400.020.6639889
CATGGGA38400.018.6867454
CCTATAC14450.018.417383
CATGGGT20050.018.0130444
AGAGTAC72700.017.41799410-11
GTATATA28000.017.3121011
Produced by FastQC (version 0.11.5)