FastQCFastQC Report
Fri 10 Feb 2017
SRR2568596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311140
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC40400.12201235828143782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.041.973041
GTATCAA49550.038.840881
TCAACGC49050.037.6690754
CAACGCA51450.035.911925
ATCAACG51950.035.7550773
AACGCAG54050.034.4454576
GTACATG99150.029.4754281
CGCAGAG61700.029.4049038
ACGCAGA62400.029.3794927
TATCAAC64100.029.2764452
ACATGGG97950.027.984523
TACATGG108850.026.0570452
CATGGGG58850.024.132324
CGATAGC1852.5457302E-723.1070824
GTATAGG14150.022.1648711
GCAGAGT82600.021.6771859
CATGGGA39950.019.2607214
TAATACT37700.018.8983674
GTATATA30450.018.415031
GGACCGT3408.949428E-1018.1595886
Produced by FastQC (version 0.11.5)