FastQCFastQC Report
Fri 10 Feb 2017
SRR2568593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791391
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.21966170422872505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.16194119541741586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.055.2642331
GTATCAA55050.045.4021721
TCAACGC67450.036.7705654
ATCAACG68150.036.3262063
CAACGCA68750.036.0051545
TATCAAC70150.035.426012
AACGCAG71100.034.9477776
CGGACTA550.002576920734.5538865
ACGCAGA81000.030.4417637
CGCAGAG81900.029.991228
GTACATG89450.027.3042961
TACATGG91000.026.0037612
GCAGAGT92950.025.5561819
ACATGGG92000.024.7914333
TGGTATC13850.022.643442
TACCGCG1100.002874377621.5955756
GTGGTAT14850.021.4386081
GAGTACT42900.021.15199712-13
CATGGGA34400.021.1322024
CAGAGTA90800.020.6151110-11
Produced by FastQC (version 0.11.5)