FastQCFastQC Report
Fri 10 Feb 2017
SRR2568593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791391
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.25030827999024224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.20079368490742666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18830.10511384728403793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.055.730451
GTATCAA55000.048.2960931
TCAACGC68100.038.5719074
ATCAACG68750.038.2816623
CAACGCA69100.038.1511845
TATCAAC70350.037.6177942
AACGCAG72350.036.4989936
ACGCAGA82700.031.529097
CGCAGAG84450.030.8194968
GCAGAGT95750.027.0831059
GTACATG85050.025.4772991
TACATGG86450.024.7864172
ACATGGG88900.023.5662563
GTGGTAT14400.023.099281
AGAGTAC88500.021.70800410-11
TATAACG1100.002873813321.596312
TGGTATC13350.021.3536552
CATGGGG52400.021.1211574
CAGAGTA93550.020.66308810-11
GTACCGT2309.0383764E-820.6498456
Produced by FastQC (version 0.11.5)