FastQCFastQC Report
Fri 10 Feb 2017
SRR2568590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222027
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.051.2466661
GTATCAA40300.041.037761
TCAACGC46150.034.804464
CAACGCA47100.034.101695
ATCAACG47500.034.0169073
AACGCAG50050.032.0880786
TATCAAC51200.031.4658342
ACGCAGA57000.028.0101287
GTACATG72500.027.9242211
CGCAGAG57350.027.6728538
TACATGG73150.026.8964842
ACATGGG74050.025.6710973
CATGGGG48950.022.6200284
GCAGAGT72050.021.7631059
GTATATA16700.020.7737981
AGAGTAC65450.019.3476610-11
AATAGCG1507.701876E-419.008165
CAGAGTA69000.018.55882810-11
GTATTAT11200.018.2456741
ATTATAC11900.017.9711173
Produced by FastQC (version 0.11.5)