FastQCFastQC Report
Fri 10 Feb 2017
SRR2568590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222027
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.048.3154531
GTATCAA41700.040.0017051
TCAACGC48450.033.8226744
CAACGCA49550.033.1676755
ATCAACG49500.033.111933
AACGCAG52550.031.5417986
TATCAAC54200.030.419332
ACGCAGA60650.027.1726787
CGCAGAG60800.026.7150658
GTACATG75300.024.613711
ACATGGG71500.024.5183323
TACATGG74700.023.9158972
GCAGAGT76200.021.4406139
CATGGGG46850.021.2908154
AGAGTAC66550.020.62450410-11
CAGAGTA70650.018.48648310-11
GTATATA18250.017.967751
GAGTACT34500.017.7584212-13
GTATACG1350.00937645817.6012571
GTATAGG8650.017.0315061
Produced by FastQC (version 0.11.5)