FastQCFastQC Report
Fri 10 Feb 2017
SRR2568589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677432
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.10301460804372398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.046.5538861
GTATCAA32300.040.7606471
TCAACGC36000.035.646114
ATCAACG36400.035.3860243
CAACGCA37200.034.3664175
AACGCAG39700.032.5594756
TATCAAC40500.032.0384522
ACGCAGA44700.028.7048457
CGCAGAG46550.027.4611428
GTACATG51250.026.4310761
CGTATAC1351.3367437E-524.6449623
TACATGG52100.024.6314452
ACATGGG53450.023.5647053
GCAGAGT55850.022.3778639
GTATATA12750.022.3668561
GTATTAG5850.020.311781
GTATAGA8250.020.1640571
AGAGTAC49950.019.74097410-11
TATACTG9950.019.105655
CATGGGG37350.019.087614
Produced by FastQC (version 0.11.5)