FastQCFastQC Report
Fri 10 Feb 2017
SRR2568589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677432
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.11237415287177065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.045.9655761
GTATCAA33250.038.439611
TCAACGC37300.033.3662344
ATCAACG37850.032.8843273
CAACGCA38200.032.704475
AACGCAG40300.031.2322856
TATCAAC40600.030.894652
GTACATG52350.027.7730161
TACATGG53000.027.2522932
ACGCAGA47200.026.3646647
CGCAGAG47700.025.9887318
CGTATAC1658.391726E-825.916531
ACATGGG55050.025.3713513
GTCCGTA1000.001651199123.7568231
CATGGGG36000.021.244094
TATACCG1353.7957024E-421.1121395
GCAGAGT59600.020.241869
GTATAGC7600.020.0057431
GTATAAG6050.019.6337361
TATACAG15050.018.306545
Produced by FastQC (version 0.11.5)