FastQCFastQC Report
Fri 10 Feb 2017
SRR2568587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656025
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.048.0184251
GTATCAA51750.040.5929071
TCAACGC60250.033.366084
CAACGCA63050.031.805345
ATCAACG63900.031.6101343
TATCAAC66600.030.8281732
AACGCAG64950.030.7274466
ACGCAGA75350.026.3607377
GTACATG99600.025.43351
CGCAGAG78650.025.0729648
TACATGG99550.024.4914442
ACATGGG102400.022.8815383
CATGGGG69550.020.6362934
GCAGAGT94950.020.5188679
AGAGTAC80850.020.15944910-11
GTATATA17500.019.282291
GTATAAA13100.018.5027871
CAGAGTA92200.017.42008610-11
GAGTACT32650.016.80951312-13
GGACCGT3151.335975E-716.5935176
Produced by FastQC (version 0.11.5)