FastQCFastQC Report
Fri 10 Feb 2017
SRR2568587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656025
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.041.702421
GTATCAA51250.039.2180441
TCAACGC56400.034.3596534
CAACGCA58800.033.199555
ATCAACG58800.033.1243863
AACGCAG60150.032.451986
TATCAAC63850.030.954432
GTACATG93900.027.3254681
ACGCAGA71600.027.1297197
CGCAGAG73450.026.3817358
TACATGG96050.025.6225742
ACATGGG95750.024.9557423
CGTTATA1801.948847E-723.7552932
CATGGGG64750.021.9330544
GCAGAGT89500.021.3323159
AGAGTAC77250.019.36631610-11
TACCGTA2702.305751E-819.349247
GTACCGT4253.6379788E-1217.8799386
CAGAGTA86450.017.74485810-11
GAGTACT36950.017.28792412-13
Produced by FastQC (version 0.11.5)