FastQCFastQC Report
Fri 10 Feb 2017
SRR2568584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4169490
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61000.039.8969731
GGTATCA36300.036.9268571
TCAACGC64900.035.59224
GTACATG157900.034.438461
CAACGCA67500.034.149195
ATCAACG70650.032.8327673
AACGCAG71400.032.546636
TACATGG165950.032.3668942
TATCAAC74700.031.625372
ACATGGG171500.030.903713
CATGGGG117550.027.1308384
ACGCAGA84500.027.1082467
CGCAGAG89400.025.568988
GACCGTA1656.303482E-520.161617
GTATAGG14950.019.7128931
ATGGGGG54250.019.7117485
GCAGAGT115300.019.4541979
CATGGGA55350.019.2350674
GAGTACA109500.019.1002221
ATGGGGA49100.018.5849825
Produced by FastQC (version 0.11.5)