FastQCFastQC Report
Fri 10 Feb 2017
SRR2568584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4169490
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62450.041.012881
GGTATCA37100.036.1192861
TCAACGC68700.035.8141484
CAACGCA70300.034.9314655
AACGCAG73150.033.632616
ATCAACG74150.033.315133
GTACATG160300.032.2225531
TATCAAC79450.031.2160552
TACATGG170550.029.724472
ACATGGG178750.027.3741423
ACGCAGA91100.027.0057687
CATGGGG120200.025.5275824
CGCAGAG97850.024.9001318
TATACTG17350.021.6276455
GCAGAGT120550.019.8567649
GAGTACA114250.019.631331
AGTACAT111150.019.3637812
GTATATA18800.019.209661
GTATAGT10450.018.6436731
TTATACT20800.018.4970864
Produced by FastQC (version 0.11.5)