FastQCFastQC Report
Fri 10 Feb 2017
SRR2568583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503858
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.038.623491
GTATCAA45200.033.0137021
GTACATG108650.032.4109341
TCAACGC45350.031.4360774
ACATGGG108450.030.3673363
ATCAACG47450.030.2464073
CAACGCA48100.029.735895
TACATGG115250.029.6477012
AACGCAG51850.027.5836936
TATCAAC56950.025.200912
CATGGGG72600.024.284084
ACGCAGA61050.023.0381157
CGCAGAG64050.021.7361778
GAGTACA76050.021.6836951
CATGGGA47350.020.0721744
TATACTG21550.020.065675
AGTACAT73800.020.0265942
GTATAGG15400.019.13261
CTATACT19700.018.815374
CCTATAC14750.017.7204073
Produced by FastQC (version 0.11.5)