FastQCFastQC Report
Fri 10 Feb 2017
SRR2568583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503858
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.039.896381
GTATCAA42900.034.5607451
GTACATG106000.032.0542761
TCAACGC45950.030.3908624
ACATGGG107100.030.0321063
TACATGG113400.029.4558982
CAACGCA47850.029.184125
ATCAACG48850.028.788153
AACGCAG52550.026.5716576
TATCAAC56700.025.140172
CATGGGG70050.024.2748244
GTATAGG17100.023.6215611
ACGCAGA60300.022.8415057
CATGGGA45050.021.7196924
CGCAGAG63600.021.5069798
TATACTG21550.020.4982555
GAGTACA72700.019.1522161
TAATACT29050.018.803254
CCTATAC13900.018.7989883
CGTATAC3557.8216544E-1118.7364043
Produced by FastQC (version 0.11.5)