FastQCFastQC Report
Fri 10 Feb 2017
SRR2568581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295012
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.047.1696931
GTATCAA34600.046.151681
TCAACGC43550.036.338044
CAACGCA46050.034.363795
ATCAACG46400.034.1075553
TATCAAC46750.034.0555232
AACGCAG48100.032.9965746
GTACATG87300.029.1793021
TACATGG90200.027.4508342
ACGCAGA56850.027.418227
ACATGGG93350.026.1681583
CGCAGAG59600.025.913358
CATGGGG64700.023.284154
GCAGAGT72900.020.7293669
AGAGTAC61050.018.99286810-11
CATGGGA31250.017.7926734
CAGAGTA67000.017.48350710-11
GTATAAG9450.017.099031
GAGTACA65500.016.9784831
GTATTAA14350.016.8905051
Produced by FastQC (version 0.11.5)