FastQCFastQC Report
Fri 10 Feb 2017
SRR2568581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295012
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.050.171521
GTATCAA37050.040.1496161
TCAACGC44200.032.884244
ATCAACG45050.032.2694053
CAACGCA45950.031.7352165
TATCAAC46650.031.575492
AACGCAG48150.030.4791976
GTACATG84650.029.3629721
TACATGG85700.028.4430792
ACATGGG88300.026.7937163
ACGCAGA56250.025.5835867
CGCAGAG58950.024.2506858
CATGGGG62450.021.2967934
GTATATA12100.020.4241141
CGTATAC2251.6665126E-619.0030943
GCAGAGT74250.018.8697819
CATGGGA32100.018.6446484
AGAGTAC62200.017.82944310-11
CAGAGTA68600.017.51611110-11
GAGTACA63900.017.0317751
Produced by FastQC (version 0.11.5)