FastQCFastQC Report
Fri 10 Feb 2017
SRR2568577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984266
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.043.0273321
GTATCAA17150.040.1765821
TCAACGC20050.033.89154
ATCAACG20800.032.669453
CAACGCA20800.032.4393425
TATCAAC21700.031.7524622
AACGCAG21400.031.5282216
GTACATG40600.030.7821431
GTGTACG1102.7243204E-630.239491
TACATGG41050.029.287112
ACATGGG41450.027.6287753
TATACGT350.0083910427.14415420-21
ACGCAGA26100.025.6686787
CATGGGG25550.024.5500354
CGCAGAG27800.024.099018
GTATAGT2452.9649527E-1023.274711
GACTGCG1452.3284485E-522.9379677
CTACACT4650.020.4383664
GTATATA5150.020.2994651
ACTGCGC1456.1326666E-419.6611168
Produced by FastQC (version 0.11.5)