##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568577_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 984266 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.231540051165034 34.0 31.0 34.0 31.0 34.0 2 32.36204542268045 34.0 31.0 34.0 31.0 34.0 3 32.50318511459301 34.0 31.0 34.0 31.0 34.0 4 35.66066896550323 37.0 35.0 37.0 35.0 37.0 5 35.69687868929741 37.0 37.0 37.0 35.0 37.0 6 35.822408779740435 37.0 37.0 37.0 35.0 37.0 7 35.86490745387934 37.0 37.0 37.0 35.0 37.0 8 35.90105012262945 37.0 37.0 37.0 35.0 37.0 9 37.70886935035854 39.0 39.0 39.0 35.0 39.0 10-11 37.71858674382738 39.0 39.0 39.0 36.0 39.0 12-13 37.707560761013795 39.0 39.0 39.0 35.0 39.0 14-15 39.23552830230852 41.0 40.0 41.0 37.0 41.0 16-17 39.23093757175398 41.0 40.0 41.0 37.0 41.0 18-19 39.245599258736966 41.0 40.0 41.0 37.0 41.0 20-21 39.24052237911296 41.0 40.0 41.0 37.0 41.0 22-23 39.172957310320584 41.0 40.0 41.0 36.5 41.0 24-25 39.10961721729695 41.0 40.0 41.0 36.0 41.0 26-27 39.036777151704925 41.0 40.0 41.0 36.0 41.0 28-29 38.95597887156521 41.0 39.0 41.0 36.0 41.0 30-31 38.859273306199746 41.0 39.0 41.0 35.5 41.0 32-33 38.747405172991854 41.0 39.0 41.0 35.0 41.0 34-35 38.66235804142376 41.0 39.0 41.0 35.0 41.0 36-37 38.5608844560312 40.0 39.0 41.0 35.0 41.0 38-39 38.45357200187754 40.0 38.0 41.0 34.5 41.0 40-41 38.331031956808424 40.0 38.0 41.0 34.0 41.0 42-43 38.21384056748887 40.0 38.0 41.0 34.0 41.0 44-45 38.06617113666428 40.0 38.0 41.0 33.0 41.0 46-47 38.03948830905467 40.0 38.0 41.0 33.5 41.0 48-49 38.181801972231085 40.0 38.0 41.0 34.0 41.0 50-51 36.70273635379054 38.5 36.0 40.0 31.5 40.5 52-53 36.91336488307023 39.0 37.0 39.5 32.5 40.5 54-55 37.724670465097844 40.0 37.0 41.0 33.0 41.0 56-57 37.71335645039045 40.0 37.0 41.0 33.0 41.0 58-59 37.51992804790575 40.0 37.0 41.0 33.0 41.0 60-61 37.28382672976614 40.0 36.0 41.0 32.5 41.0 62-63 36.99807521543973 39.0 35.5 41.0 32.0 41.0 64-65 36.70622372407459 39.0 35.0 41.0 31.5 41.0 66-67 36.58700442766488 39.0 35.0 41.0 32.0 41.0 68-69 36.27168621084138 37.5 35.0 40.5 31.5 41.0 70-71 35.90724458632118 37.0 35.0 39.5 31.0 41.0 72-73 35.556258673976345 36.5 35.0 39.0 31.0 41.0 74-75 35.1738762692199 36.0 35.0 39.0 31.0 41.0 76-77 34.80719744459323 35.5 35.0 37.5 30.5 39.5 78-79 34.459498245393014 35.0 35.0 37.0 30.0 39.0 80-81 34.15207576000797 35.0 35.0 37.0 30.0 39.0 82-83 33.86267888964975 35.0 34.0 36.0 30.0 37.5 84-85 33.609081792929956 35.0 34.0 36.0 29.5 37.0 86-87 33.41560919507532 35.0 34.0 35.5 29.0 37.0 88-89 33.27181219304538 35.0 34.0 35.0 29.0 36.0 90-91 33.09909516329935 35.0 34.0 35.0 29.0 36.0 92-93 32.986835367675 35.0 34.0 35.0 29.0 36.0 94-95 32.892708373549425 35.0 34.0 35.0 29.0 36.0 96-97 32.793126045195095 35.0 34.0 35.0 29.0 35.0 98-99 32.72813497570779 35.0 34.0 35.0 28.5 35.0 100-101 31.480276165182985 34.5 31.5 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.028795963667519686 1101 2 0.13545841625302302 1101 3 0.08418729650310297 1101 4 -0.027694091799887133 1101 5 0.1701645576549069 1101 6 0.16403924195974895 1101 7 0.0872655762305996 1101 8 0.11260624802986285 1101 9 0.2094144614278335 1101 10-11 0.3186961551684817 1101 12-13 0.25155078501867933 1101 14-15 0.36283964429635773 1101 16-17 0.38230567716929187 1101 18-19 0.3452252697119391 1101 20-21 0.28710977365320645 1101 22-23 0.25029741605229106 1101 24-25 0.26931262094045394 1101 26-27 0.20141979223891582 1101 28-29 0.2568533114289693 1101 30-31 0.27855832944517545 1101 32-33 0.18032913503902392 1101 34-35 0.1722432182354865 1101 36-37 0.2427066431685958 1101 38-39 0.27038099042725605 1101 40-41 0.14205790984574662 1101 42-43 0.026360043452726245 1101 44-45 0.03904685635023242 1101 46-47 0.16137314400677383 1101 48-49 0.30369995518515225 1101 50-51 0.1352286760644006 1101 52-53 0.16790771043319097 1101 54-55 0.10189295488904548 1101 56-57 0.2197657004046576 1101 58-59 0.22606212210565957 1101 60-61 0.16601451757743035 1101 62-63 0.2278188369604095 1101 64-65 0.15526620491579735 1101 66-67 0.15004760257117056 1101 68-69 0.19070828347198443 1101 70-71 0.1649976419459449 1101 72-73 0.16307242513479991 1101 74-75 0.09049737704271621 1101 76-77 0.23568857658747078 1101 78-79 0.2617957408171634 1101 80-81 0.20668881126329808 1101 82-83 0.27477499491648416 1101 84-85 0.26242950544745014 1101 86-87 0.13759009537202616 1101 88-89 0.030088043004916187 1101 90-91 0.07010928762240098 1101 92-93 0.09818803955685951 1101 94-95 0.12609396745912704 1101 96-97 0.2606068548961957 1101 98-99 0.10772535825293517 1101 100-101 0.0018220785990479271 1102 1 0.14571170613526618 1102 2 0.18426695422579797 1102 3 0.2830155696377403 1102 4 0.05435000661278622 1102 5 0.038743858178854396 1102 6 0.08212051986952673 1102 7 0.049579930271661965 1102 8 0.05855512235170579 1102 9 0.24447649175033348 1102 10-11 0.33476522049573987 1102 12-13 0.38305274692594793 1102 14-15 0.4863330001251924 1102 16-17 0.4979204468096512 1102 18-19 0.44810195977702705 1102 20-21 0.48617409480232965 1102 22-23 0.5486760370675228 1102 24-25 0.55878487949429 1102 26-27 0.6448557226163629 1102 28-29 0.505222258345249 1102 30-31 0.3934672768258167 1102 32-33 0.4615791834095191 1102 34-35 0.4903686100364979 1102 36-37 0.41716083050558694 1102 38-39 0.32399639468849983 1102 40-41 0.36095645563518985 1102 42-43 0.4166170649137513 1102 44-45 0.4457297239079523 1102 46-47 0.44726289023580534 1102 48-49 0.5121822051483917 1102 50-51 0.44575951322222807 1102 52-53 0.5487607337826006 1102 54-55 0.6492836569190601 1102 56-57 0.5762221940468422 1102 58-59 0.6109782670159234 1102 60-61 0.5855856067261769 1102 62-63 0.5549411407890332 1102 64-65 0.5061682179023066 1102 66-67 0.571288828163425 1102 68-69 0.5928079499090515 1102 70-71 0.4409532494406605 1102 72-73 0.5676068687967231 1102 74-75 0.5035558627753574 1102 76-77 0.4177024918114043 1102 78-79 0.32072183844042 1102 80-81 0.4051587557726677 1102 82-83 0.30053363775649444 1102 84-85 0.39707903219052554 1102 86-87 0.38518821008361925 1102 88-89 0.44474314202899023 1102 90-91 0.3590173527252105 1102 92-93 0.37497639348957534 1102 94-95 0.45613088382074096 1102 96-97 0.35293103782180424 1102 98-99 0.2713902165080384 1102 100-101 0.21916270749287037 1103 1 0.02785022375373103 1103 2 0.08341640744572487 1103 3 0.14449795116872366 1103 4 0.0895869446443811 1103 5 0.03752879135018361 1103 6 -0.0017190913090843196 1103 7 0.02892379418418045 1103 8 0.20679327545012427 1103 9 0.28214356343927705 1103 10-11 0.417997298260012 1103 12-13 0.43651568738767565 1103 14-15 0.5003062686549669 1103 16-17 0.515538915825438 1103 18-19 0.5070327009677911 1103 20-21 0.5122980316188617 1103 22-23 0.4891377624624198 1103 24-25 0.5144349402476323 1103 26-27 0.5555483107087085 1103 28-29 0.4845661222577604 1103 30-31 0.3752412743956768 1103 32-33 0.453681249023127 1103 34-35 0.4539166051762251 1103 36-37 0.4548279021945305 1103 38-39 0.42909967536893845 1103 40-41 0.3810050583083324 1103 42-43 0.5569572836257777 1103 44-45 0.6030808782214407 1103 46-47 0.6033989777206159 1103 48-49 0.6810764943342988 1103 50-51 0.6134387355794573 1103 52-53 0.672697550307511 1103 54-55 0.6687247261778637 1103 56-57 0.6466960701100248 1103 58-59 0.6188762014023084 1103 60-61 0.6439088145026091 1103 62-63 0.7384162319190395 1103 64-65 0.5802872944515158 1103 66-67 0.5475950250042487 1103 68-69 0.5241566740888786 1103 70-71 0.5217551935475839 1103 72-73 0.46007345445892867 1103 74-75 0.3814416464934638 1103 76-77 0.47055789885879307 1103 78-79 0.5260681324865999 1103 80-81 0.4719874313498238 1103 82-83 0.3612869791902682 1103 84-85 0.38492836390376794 1103 86-87 0.2509233255149681 1103 88-89 0.38216720035219964 1103 90-91 0.4811315690071041 1103 92-93 0.42540166687960834 1103 94-95 0.41907134554613634 1103 96-97 0.5522019977245947 1103 98-99 0.35097709378628394 1103 100-101 0.3977775313081793 1104 1 0.0836662448544061 1104 2 0.13747799058889854 1104 3 0.06150592319254855 1104 4 0.04715444012492753 1104 5 -0.059763980483978685 1104 6 0.07200879299283969 1104 7 0.10778755852048505 1104 8 0.16816007745350703 1104 9 0.18579320929174514 1104 10-11 0.321628421752294 1104 12-13 0.34139077259052897 1104 14-15 0.3835946543942299 1104 16-17 0.361663297178616 1104 18-19 0.2781637503706307 1104 20-21 0.2590143876038056 1104 22-23 0.28671577864355413 1104 24-25 0.4557775890933229 1104 26-27 0.37661786587569424 1104 28-29 0.32220207638157916 1104 30-31 0.44689761793360105 1104 32-33 0.31724850741746735 1104 34-35 0.306168183281315 1104 36-37 0.28841116390807287 1104 38-39 0.27804760530996475 1104 40-41 0.37206024012382244 1104 42-43 0.3829663818091049 1104 44-45 0.34636392952096884 1104 46-47 0.33802764144346753 1104 48-49 0.2604499940231193 1104 50-51 0.18111573525346358 1104 52-53 0.10903323474575899 1104 54-55 0.2970498602797562 1104 56-57 0.30722492053825334 1104 58-59 0.41032109367626646 1104 60-61 0.4016289342311481 1104 62-63 0.3503720449765666 1104 64-65 0.2636979277680567 1104 66-67 0.45665394092755207 1104 68-69 0.47219034033077634 1104 70-71 0.4758256674236492 1104 72-73 0.5046678888601335 1104 74-75 0.3396196389659778 1104 76-77 0.3069950858308985 1104 78-79 0.24131648029207042 1104 80-81 0.27371149263660755 1104 82-83 0.19504064661759202 1104 84-85 0.18491280512120767 1104 86-87 0.23752795462322496 1104 88-89 0.20287816262585778 1104 90-91 0.289990443440324 1104 92-93 0.15256507855018242 1104 94-95 0.037958966682367645 1104 96-97 0.1400965239967107 1104 98-99 0.189075366020532 1104 100-101 0.032069088075509455 1105 1 -0.07852887709681511 1105 2 -0.09897187390974693 1105 3 -0.08323662423292433 1105 4 -0.2755284867043457 1105 5 -0.2213357962021334 1105 6 -0.14178524494753475 1105 7 -0.13717179107300836 1105 8 -0.0034659388066557995 1105 9 -0.023204080681153982 1105 10-11 0.04160132148129492 1105 12-13 0.14399239860679103 1105 14-15 0.19592527770046075 1105 16-17 0.19265245707021705 1105 18-19 0.1917680864139939 1105 20-21 0.05982739573388329 1105 22-23 -0.0644088826030611 1105 24-25 -0.03755574424001651 1105 26-27 0.0498698983960324 1105 28-29 0.06018310619187872 1105 30-31 0.12028515180893606 1105 32-33 0.05085284346082375 1105 34-35 -0.07929252132573339 1105 36-37 -0.04681918839545318 1105 38-39 -0.05555673073339307 1105 40-41 0.07869980651948794 1105 42-43 -0.14997399759469232 1105 44-45 -0.20095314364976247 1105 46-47 -0.28676910652401233 1105 48-49 -0.20045786505546914 1105 50-51 -0.08494117531564171 1105 52-53 -0.023500640592992283 1105 54-55 0.05707696055075928 1105 56-57 0.010476953058578431 1105 58-59 -0.05772768681153906 1105 60-61 -0.1329239112973042 1105 62-63 -0.055617114915030186 1105 64-65 -0.027508034291564343 1105 66-67 0.13649133930153567 1105 68-69 -0.010045432026949186 1105 70-71 -0.06695211035412996 1105 72-73 -0.1618497263160137 1105 74-75 -0.12609851821559914 1105 76-77 -0.05282876240757872 1105 78-79 -0.30070248989763115 1105 80-81 -0.1331448759270728 1105 82-83 -0.10804878427671838 1105 84-85 -0.18470779108475455 1105 86-87 -0.24267529740929916 1105 88-89 -0.2254958211139808 1105 90-91 -0.43862744273853593 1105 92-93 -0.31981974529806223 1105 94-95 -0.35354509835828196 1105 96-97 -0.10396851665369411 1105 98-99 -0.33921731690629997 1105 100-101 -0.5446924295396691 1106 1 0.1739251106332489 1106 2 0.18715627878282248 1106 3 0.2711123873553518 1106 4 0.22441016360240695 1106 5 0.2791481847112962 1106 6 0.22227065143964353 1106 7 0.3223169614832244 1106 8 0.409069848514406 1106 9 0.4232240038450925 1106 10-11 0.5029953228446189 1106 12-13 0.5510271634329698 1106 14-15 0.5200902402089795 1106 16-17 0.5397008551615556 1106 18-19 0.44102848177868026 1106 20-21 0.4020564345886939 1106 22-23 0.4687335850179366 1106 24-25 0.451943233265105 1106 26-27 0.4362093666986837 1106 28-29 0.49638283352658163 1106 30-31 0.602875472190668 1106 32-33 0.6569537296230621 1106 34-35 0.5525956858999024 1106 36-37 0.6709040563216675 1106 38-39 0.6616552665399595 1106 40-41 0.5242860369216658 1106 42-43 0.6572107331483252 1106 44-45 0.8415008440803007 1106 46-47 0.7053065580976607 1106 48-49 0.5760417941129035 1106 50-51 0.5755905220258697 1106 52-53 0.6626206954669911 1106 54-55 0.5966645542794566 1106 56-57 0.7755587526495873 1106 58-59 0.6480698592007315 1106 60-61 0.6838450048581066 1106 62-63 0.6766289655212248 1106 64-65 0.7120557012230861 1106 66-67 0.5943952247821116 1106 68-69 0.6331958767665 1106 70-71 0.7561937655831628 1106 72-73 0.709987356165243 1106 74-75 0.7630971191395517 1106 76-77 0.5967451980559488 1106 78-79 0.6438714705658981 1106 80-81 0.6849975916939144 1106 82-83 0.5119716656210258 1106 84-85 0.5836596254114923 1106 86-87 0.6679484244720939 1106 88-89 0.5027659375772231 1106 90-91 0.4341435184066569 1106 92-93 0.5743554421593302 1106 94-95 0.5922422227051172 1106 96-97 0.688843344287001 1106 98-99 0.6904145849341887 1106 100-101 0.7891691928188891 1107 1 0.27250205073823963 1107 2 0.18837062322656095 1107 3 0.19977597599617525 1107 4 0.2245746060791589 1107 5 0.22538814423314335 1107 6 0.15458359812233624 1107 7 0.17351549415035805 1107 8 0.18131069349574602 1107 9 0.16498606823064677 1107 10-11 0.13820498700248152 1107 12-13 0.17993741578892042 1107 14-15 0.20337086762027923 1107 16-17 0.15339701606219336 1107 18-19 0.1306970669409111 1107 20-21 0.21171743009814747 1107 22-23 0.159565916938746 1107 24-25 0.24005593960878713 1107 26-27 0.17969807709018681 1107 28-29 0.1348623730876426 1107 30-31 0.2601362400105387 1107 32-33 0.22519419512175887 1107 34-35 0.2261141224314258 1107 36-37 0.10444083451405817 1107 38-39 0.09020501038916962 1107 40-41 0.14894608372452467 1107 42-43 0.1223742269184811 1107 44-45 0.10657262954611468 1107 46-47 0.1437291752629335 1107 48-49 0.19785255877042118 1107 50-51 0.07811724392834662 1107 52-53 0.10932868357654257 1107 54-55 0.1309887589470904 1107 56-57 0.14973812144346965 1107 58-59 0.13356097298227354 1107 60-61 0.04937849420097251 1107 62-63 0.2184321405259695 1107 64-65 0.14065920511279018 1107 66-67 0.11049477572765198 1107 68-69 0.19272089099710144 1107 70-71 -0.03912278618459908 1107 72-73 -0.06652899321177586 1107 74-75 -0.0506116911823824 1107 76-77 -0.22639691819222918 1107 78-79 -0.045765491043113116 1107 80-81 -0.1061288392718609 1107 82-83 0.037861868644242236 1107 84-85 -0.154633335185558 1107 86-87 -0.09729729053643865 1107 88-89 -0.12264358743700399 1107 90-91 -0.11108448741207866 1107 92-93 -0.01678996293838253 1107 94-95 0.05009751332556789 1107 96-97 0.1366328579167515 1107 98-99 0.11768989958854092 1107 100-101 0.02681387057487683 1108 1 0.41447315130075424 1108 2 0.3103024105166625 1108 3 0.35062183869247576 1108 4 0.4812463611658373 1108 5 0.4030297661147486 1108 6 0.3750662312751345 1108 7 0.33320684313929405 1108 8 0.33313311460781136 1108 9 0.3768657863461158 1108 10-11 0.34807303660412003 1108 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82-83 -0.058541725171672 2316 84-85 -0.2540349825978794 2316 86-87 -0.08504308874693578 2316 88-89 -0.08823331812283897 2316 90-91 -0.09332114936691482 2316 92-93 -0.1776561495414981 2316 94-95 -0.22643574205294215 2316 96-97 -0.20082542021913952 2316 98-99 -0.1279969292537544 2316 100-101 0.04880083041087602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1474.0 3 65.0 4 32.0 5 34.0 6 294.0 7 1621.0 8 1302.0 9 568.0 10 430.0 11 576.0 12 915.0 13 1143.0 14 1276.0 15 1457.0 16 1607.0 17 1791.0 18 2088.0 19 2518.0 20 2960.0 21 3580.0 22 4215.0 23 4460.0 24 4595.0 25 4508.0 26 4876.0 27 5279.0 28 6231.0 29 7149.0 30 8768.0 31 10842.0 32 13858.0 33 18371.0 34 28169.0 35 48037.0 36 102510.0 37 240198.0 38 350660.0 39 95777.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.624902222801644 18.185234828238055 12.725498663711624 24.46436428524868 2 15.229562813645536 19.74575295808417 40.73646288810055 24.28822134016975 3 18.54124923729478 25.566897523996566 29.30081704955572 26.591036189152934 4 12.202008678468841 16.590848900931 38.64200297431092 32.565139446289244 5 13.387554301603712 38.17709579999695 34.9482829743783 13.487066924021043 6 31.469500688919144 36.97464186897196 18.514867516723985 13.040989925384913 7 28.54030922648569 32.03545248458654 21.935486499092608 17.48875178983516 8 26.617897621668856 35.22329507784342 19.943232913096658 18.21557438739107 9 27.214930124516144 15.679645118288812 19.168989399782678 37.936435357412364 10-11 24.935564451332958 25.79830323964932 28.825933895389866 20.440198413627854 12-13 26.133212346003393 23.075125188278893 28.446331222165533 22.34533124355218 14-15 23.22748259372249 24.468493020939473 25.747287278815644 26.556737106522394 16-17 23.06827504636425 27.673960087048748 26.10673650652764 23.15102836005936 18-19 23.020684636269486 26.947432168693396 27.548047440565703 22.483835754471407 20-21 24.034986033981518 26.075261293544894 27.288719762132423 22.601032910341164 22-23 23.64321811373611 26.086797191080148 27.048654250102178 23.221330445081563 24-25 23.272582898765396 26.223318292065283 27.27238947467544 23.23170933449388 26-27 23.232818363998874 26.50582846334311 27.075262282122974 23.18609089053504 28-29 23.27292995413755 26.399721058552256 27.103946410664932 23.22340257664527 30-31 23.420979226009532 26.3388157827761 27.065080842035822 23.17512414917855 32-33 23.2986947412998 26.472869716410102 26.931165978170355 23.297269564119738 34-35 23.46679749126196 26.426483430807156 27.023998306337187 23.082720771593692 36-37 23.53452065500104 26.301902075472466 26.915100598748776 23.248476670777723 38-39 23.401869938890087 26.481386107978487 26.905715262821477 23.211028690309945 40-41 23.544964391190103 26.378658324360742 26.916657677112866 23.15971960733629 42-43 23.3935826237824 26.244031249920504 26.922503555174526 23.43988257112257 44-45 23.450423418831072 26.556190115716504 26.789291771847303 23.204094693605118 46-47 23.5057089392393 26.452012043668983 26.62962461727568 23.412654399816027 48-49 23.445587353144543 26.46667205982539 26.69720415598847 23.390536431041596 50-51 23.627593891428614 26.645907903339467 26.431756837544867 23.294741367687053 52-53 23.492445371228346 26.535770313639347 26.606093264923654 23.365691050208653 54-55 23.34899035134145 26.48495704922037 26.72856532182551 23.43748727761267 56-57 23.45115089930547 26.68696108844867 26.55236042241709 23.309527589828768 58-59 23.45979789014246 26.53297517599111 26.706065331482154 23.301161602384276 60-61 23.491759862485374 26.731976273942003 26.615158065979127 23.161105797593496 62-63 23.329194000681973 26.69757594572724 26.74796044562291 23.225269607967878 64-65 23.402543431673458 26.52221418709475 26.7166687956079 23.358573585623894 66-67 23.3312030805897 26.71911905229533 26.577209619922883 23.372468247192085 68-69 23.326912960707524 26.487394804076885 26.714956822739715 23.470735412475875 70-71 23.387365953038532 26.313349846032985 26.688956097521267 23.61032810340722 72-73 23.25756642918274 26.599352982660385 26.544654678155176 23.5984259100017 74-75 23.354273703757812 26.602177588119325 26.634196451251153 23.40935225687171 76-77 23.248447521123893 26.450575180698362 26.674946554005906 23.626030744171842 78-79 23.226733989352795 26.378132141644407 26.716120128027143 23.67901374097566 80-81 23.29942595174574 26.674332860432003 26.394053558493415 23.63218762932884 82-83 23.308250009289804 26.55204658239498 26.705570167557557 23.434133240757664 84-85 23.31504079937457 26.576344099156323 26.544126030163852 23.564489071305253 86-87 23.24505737767487 26.591893336047427 26.58385282817231 23.579196458105393 88-89 23.187022318111968 26.485431656506652 26.669685836700218 23.657860188681166 90-91 23.123988184333356 26.49819946416379 26.78819817188885 23.589614179614006 92-93 23.216631577447792 26.42828723569881 26.791916013810464 23.563165173042933 94-95 23.253058207055997 26.36664701042866 26.842365281419156 23.537929501096187 96-97 23.156228325368687 26.365714861463008 26.794793050683847 23.68326376248446 98-99 23.084798267520696 26.39868395075297 26.704747831856697 23.81176994986964 100-101 23.12765319640664 26.175473204783884 26.90280719058532 23.79406640822415 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1364.0 1 705.5 2 55.0 3 55.5 4 41.5 5 38.5 6 50.5 7 93.0 8 165.0 9 177.5 10 154.0 11 145.0 12 149.5 13 189.0 14 206.0 15 211.0 16 243.5 17 284.0 18 303.5 19 316.0 20 393.0 21 467.5 22 553.0 23 720.5 24 923.5 25 1188.5 26 1564.0 27 2175.0 28 3039.0 29 4215.5 30 5939.5 31 8145.0 32 10754.0 33 13493.5 34 15789.5 35 18357.0 36 22277.0 37 25629.0 38 27951.0 39 30240.5 40 32094.0 41 33421.5 42 34460.0 43 35983.5 44 37813.5 45 39520.0 46 40795.0 47 41506.5 48 41732.0 49 41233.0 50 40538.5 51 39217.5 52 37685.5 53 36983.5 54 35692.0 55 33501.5 56 30442.0 57 26846.5 58 24291.0 59 21940.5 60 18538.0 61 15222.5 62 12237.0 63 9047.0 64 6503.0 65 4800.0 66 3482.0 67 2566.0 68 1923.5 69 1441.5 70 1112.5 71 864.0 72 641.0 73 506.5 74 408.0 75 306.0 76 220.0 77 145.0 78 106.5 79 94.0 80 75.5 81 53.0 82 43.0 83 35.0 84 27.5 85 22.0 86 14.5 87 10.5 88 10.5 89 7.5 90 3.5 91 4.5 92 3.0 93 2.5 94 4.0 95 2.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24810366303417977 2 0.25044042972123387 3 0.26039708777911663 4 0.25663794136950785 5 0.25206600654701067 6 0.2329654788441336 7 0.2369278223569645 8 0.23967098325046277 9 0.23418466146346617 10-11 0.23133990201835683 12-13 0.23824860352790808 14-15 0.24022977528432354 16-17 0.18572215234499617 18-19 0.18018503128219404 20-21 0.1729715341178096 22-23 0.19258005457874192 24-25 0.19999674884634847 26-27 0.20060634015601472 28-29 0.20126673074148654 30-31 0.20116513218987553 32-33 0.1962884017125452 34-35 0.18084542186766586 36-37 0.15747775499712477 38-39 0.18023583055799955 40-41 0.1670280188485633 42-43 0.15681736441165295 44-45 0.14559072445863214 46-47 0.1533122143810718 48-49 0.15641097020520878 50-51 0.1573253571697082 52-53 0.1682980007437014 54-55 0.1773910711128902 56-57 0.17576549428711344 58-59 0.18592534944821826 60-61 0.20019994594957052 62-63 0.18501096248371882 64-65 0.18028662983380508 66-67 0.16225288692284404 68-69 0.14802908969729728 70-71 0.1613384999583446 72-73 0.16286247823251032 74-75 0.20649905614945555 76-77 0.19974275246732082 78-79 0.21645571420733828 80-81 0.20639745759784447 82-83 0.20385749380756826 84-85 0.19283405095776956 86-87 0.17688307835483497 88-89 0.16824720146789587 90-91 0.1527026230714055 92-93 0.15575057961973693 94-95 0.15661416730843084 96-97 0.17215874570492123 98-99 0.1654024420227865 100-101 0.18719533134335636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 984266.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.366148727378416 #Duplication Level Percentage of deduplicated Percentage of total 1 71.81390537035908 41.915010815402574 2 15.15254607552816 17.687915156854615 3 5.651756308994729 9.89613747905057 4 2.724494084896617 6.360729078637547 5 1.4752078085480724 4.30510991787534 6 0.906057523037863 3.172985288711281 7 0.5328874256959049 2.17718107201819 8 0.3986955398455724 1.8616258540455282 9 0.27327639173810164 1.4355081471480629 >10 1.0428443785451602 9.533156635120987 >50 0.019763381039674642 0.7774877471174121 >100 0.008216091054888708 0.7151290511534952 >500 3.496207161749759E-4 0.16202375686442314 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0159855161104823E-4 0.0 0.0 0.0 6 0.0 1.0159855161104823E-4 0.0 0.0 0.0 7 0.0 1.0159855161104823E-4 0.0 0.0 0.0 8 0.0 1.0159855161104823E-4 0.0 0.0 0.0 9 0.0 1.0159855161104823E-4 0.0 0.0 0.0 10-11 0.0 1.0159855161104823E-4 0.0 0.0 0.0 12-13 0.0 1.0159855161104823E-4 0.0 0.0 0.0 14-15 0.0 1.0159855161104823E-4 0.0 0.0 0.0 16-17 0.0 1.0159855161104823E-4 0.0 5.0799275805524116E-5 0.0 18-19 0.0 3.047956548331447E-4 0.0 1.0159855161104823E-4 0.0 20-21 0.0 5.587920338607653E-4 0.0 2.0319710322209646E-4 0.0 22-23 0.0 6.095913096662894E-4 0.0 4.5719348224971705E-4 0.0 24-25 0.0 6.095913096662894E-4 0.0 6.603905854718135E-4 0.0 26-27 0.0 6.095913096662894E-4 0.0 9.143869644994341E-4 0.0 28-29 0.0 6.095913096662894E-4 0.0 0.0013715804467491512 0.0 30-31 0.0 7.111898612773376E-4 0.0 0.0021843688596375373 0.0 32-33 0.0 7.111898612773376E-4 0.0 0.004724332649913743 0.0 34-35 0.0 7.619891370828618E-4 0.0 0.007315095715995473 0.0 36-37 0.0 8.127884128883859E-4 0.0 0.012953815330408649 0.0 38-39 0.0 8.127884128883859E-4 0.0 0.023266068318930046 0.0 40-41 0.0 8.127884128883859E-4 0.0 0.03825185468155966 0.0 42-43 0.0 8.127884128883859E-4 0.0 0.05272964828613403 0.0 44-45 0.0 8.127884128883859E-4 0.0 0.07030619771484536 0.0 46-47 0.0 8.127884128883859E-4 0.0 0.09240388269024837 0.0 48-49 0.0 8.127884128883859E-4 0.0 0.1171431300075386 0.0 50-51 0.0 8.127884128883859E-4 0.0 0.14863868100696356 0.0 52-53 0.0 8.127884128883859E-4 0.0 0.1819630059353874 0.0 54-55 0.0 0.0010667847919160064 0.0 0.21584612289767197 0.0 56-57 0.0 0.0012191826193325788 0.0 0.25602835005984154 0.0 58-59 0.0 0.0013207811709436271 0.0 0.302204891767063 0.0 60-61 0.0 0.0013207811709436271 0.0 0.3579824966015285 0.0 62-63 0.0 0.0013207811709436271 0.0 0.41909402539557394 0.0 64-65 0.0 0.0013207811709436271 0.0 0.48442189408147796 0.0 66-67 0.0 0.0013207811709436271 0.0 0.5609256034445973 0.0 68-69 0.0 0.0013715804467491512 0.0 0.6511959165510137 0.0 70-71 0.0 0.0015239782741657236 0.0 0.7555884283313656 0.0 72-73 0.0 0.0016255768257767717 0.0 0.8709027844099055 0.0 74-75 0.0 0.0016255768257767717 0.0 0.989976286898054 0.0 76-77 0.0 0.0016255768257767717 0.0 1.1220544039924167 0.0 78-79 0.0 0.0016255768257767717 0.0 1.275214220546072 0.0 80-81 0.0 0.0016255768257767717 0.0 1.4440202140478284 0.0 82-83 0.0 0.0016255768257767717 0.0 1.6285739830492978 0.0 84-85 0.0 0.0016255768257767717 0.0 1.8309074985827003 0.0 86-87 0.0 0.0016255768257767717 0.0 2.0430960736223747 0.0 88-89 0.0 0.0016255768257767717 0.0 2.2792619068422564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1005 0.0 43.027332 1 GTATCAA 1715 0.0 40.176582 1 TCAACGC 2005 0.0 33.8915 4 ATCAACG 2080 0.0 32.66945 3 CAACGCA 2080 0.0 32.439342 5 TATCAAC 2170 0.0 31.752462 2 AACGCAG 2140 0.0 31.528221 6 GTACATG 4060 0.0 30.782143 1 GTGTACG 110 2.7243204E-6 30.23949 1 TACATGG 4105 0.0 29.28711 2 ACATGGG 4145 0.0 27.628775 3 TATACGT 35 0.00839104 27.144154 20-21 ACGCAGA 2610 0.0 25.668678 7 CATGGGG 2555 0.0 24.550035 4 CGCAGAG 2780 0.0 24.09901 8 GTATAGT 245 2.9649527E-10 23.27471 1 GACTGCG 145 2.3284485E-5 22.937967 7 CTACACT 465 0.0 20.438366 4 GTATATA 515 0.0 20.299465 1 ACTGCGC 145 6.1326666E-4 19.661116 8 >>END_MODULE