FastQCFastQC Report
Fri 10 Feb 2017
SRR2568577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984266
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.11196160387537514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG250.00501852357.0072864
GGTATCA10300.049.8375131
GTATCAA19200.047.5302241
TCAACGC23050.038.5407184
ATCAACG23650.037.7676543
CAACGCA23550.037.7224435
TATCAAC26000.035.088542
AACGCAG25450.034.8991366
ACGCAGA28550.031.1097377
GTACATG37600.030.338441
CGCAGAG29900.029.7051188
TACATGG38100.028.808752
ACATGGG38850.027.271383
CATGGGG24650.024.861194
ATAACGC1151.2795751E-424.7882963
GCAGAGT35950.023.9133579
TATAACG1000.00165029423.7578642
TACCGTC1050.00219696222.6173427
CCTATAC2509.460564E-920.9047973
AGAGTAC30900.020.82771910-11
Produced by FastQC (version 0.11.5)