FastQCFastQC Report
Fri 10 Feb 2017
SRR2568576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872245
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.043.9426231
GTATCAA35250.042.3364831
TCAACGC38250.038.019284
CAACGCA39150.037.0225945
ATCAACG40300.036.2057533
AACGCAG41700.034.871376
GTACATG87250.033.3917581
TATCAAC47400.031.0833342
TACATGG91150.030.711632
ACATGGG90600.030.163653
ACGCAGA49800.028.6274787
CGCAGAG52900.027.1295418
CATGGGG60800.025.1690144
GCAGAGT68350.020.3713729
GAGTACA60050.019.7866041
ATGGGGG30600.019.4127625
CATGGGA34550.018.982134
GTATAGC12850.018.8630221
ATAGCCC9950.018.6288383
GTATAAG10550.018.0198751
Produced by FastQC (version 0.11.5)