FastQCFastQC Report
Fri 10 Feb 2017
SRR2568576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872245
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.043.3963931
GTATCAA36100.038.7040371
TCAACGC40300.032.8848574
CAACGCA41700.031.780815
GTACATG87700.031.6467691
ATCAACG42750.031.114593
TACATGG89550.029.9242842
ACATGGG88300.029.6975423
AACGCAG45050.029.5188566
TATCAAC49300.027.2740572
ACGCAGA54400.024.2706957
CATGGGG60950.024.2372574
CGCAGAG55550.023.5117478
GTATATA18650.020.8954221
CATGGGA35950.020.7442424
GTATAAT15650.019.1312071
GTATTAC7200.017.8216291
GAGTACA59550.017.7168851
GCAGAGT75300.017.344999
AGAGTAC62200.017.25656510-11
Produced by FastQC (version 0.11.5)