FastQCFastQC Report
Fri 10 Feb 2017
SRR2568574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930641
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37090.19211236060976639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.14782655087092836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.059.4847641
GTATCAA68250.044.0040661
ATCAACG91500.032.6669043
TATCAAC93200.032.1220362
TCAACGC92950.032.1061824
CAACGCA93850.031.697025
AACGCAG95400.031.378836
ACGCAGA107650.027.8522267
CGCAGAG108450.027.4276988
GTACATG137700.027.2973791
TACATGG138600.026.6744062
ACATGGG141500.025.1873933
GCAGAGT121450.023.9049729
CATGGGG74650.023.7441944
AGAGTAC115400.021.74065610-11
CATGGGA66550.020.4933174
ATGGGGG32500.019.7391685
CAGAGTA120750.019.63622310-11
GTATAAT11250.019.0080851
ATGGGAT26550.018.7933045
Produced by FastQC (version 0.11.5)