##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2568574_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1930641
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40595947149159	34.0	31.0	34.0	31.0	34.0
2	32.51902451051231	34.0	31.0	34.0	31.0	34.0
3	32.652654740057834	34.0	33.0	34.0	31.0	34.0
4	35.81969770661661	37.0	37.0	37.0	35.0	37.0
5	35.86740983952998	37.0	37.0	37.0	35.0	37.0
6	35.990229669835045	37.0	37.0	37.0	35.0	37.0
7	36.02948191818158	37.0	37.0	37.0	35.0	37.0
8	36.0672320747358	37.0	37.0	37.0	35.0	37.0
9	37.900034237333614	39.0	39.0	39.0	37.0	39.0
10-11	37.91244281044482	39.0	39.0	39.0	37.0	39.0
12-13	37.89828948002244	39.0	39.0	39.0	37.0	39.0
14-15	39.4166344234894	41.0	40.0	41.0	37.0	41.0
16-17	39.42272307487514	41.0	40.0	41.0	37.0	41.0
18-19	39.4755824619906	41.0	40.0	41.0	37.5	41.0
20-21	39.48471621601323	41.0	40.0	41.0	37.0	41.0
22-23	39.407528121489186	41.0	40.0	41.0	37.0	41.0
24-25	39.33937718094664	41.0	40.0	41.0	37.0	41.0
26-27	39.2669476096281	41.0	40.0	41.0	37.0	41.0
28-29	39.184745118331165	41.0	40.0	41.0	36.0	41.0
30-31	39.05960326130027	41.0	39.5	41.0	36.0	41.0
32-33	38.91914265780122	41.0	39.0	41.0	35.0	41.0
34-35	38.81605927772175	41.0	39.0	41.0	35.0	41.0
36-37	38.700589337945274	41.0	39.0	41.0	35.0	41.0
38-39	38.580680198959826	40.5	39.0	41.0	35.0	41.0
40-41	38.450378138659644	40.0	38.5	41.0	35.0	41.0
42-43	38.306223166295545	40.0	38.0	41.0	34.5	41.0
44-45	38.135438955248546	40.0	38.0	41.0	34.0	41.0
46-47	38.05462123719532	40.0	38.0	41.0	33.5	41.0
48-49	38.146501343336226	40.5	38.5	41.0	34.0	41.0
50-51	36.68679081196349	38.5	36.5	40.0	32.0	40.5
52-53	36.87676476361996	39.5	37.5	39.5	32.5	40.5
54-55	37.69126808142995	40.0	38.0	41.0	33.0	41.0
56-57	37.688923523327226	40.0	37.5	41.0	33.0	41.0
58-59	37.512168756387126	40.0	37.0	41.0	33.0	41.0
60-61	37.29786609732208	40.0	36.5	41.0	32.5	41.0
62-63	37.02818804738944	39.5	36.0	41.0	32.0	41.0
64-65	36.76344281510649	39.0	35.0	41.0	31.5	41.0
66-67	36.66688369303252	39.0	35.0	41.0	32.0	41.0
68-69	36.36578758039428	38.5	35.0	41.0	31.5	41.0
70-71	36.01313346189167	37.0	35.0	40.0	31.0	41.0
72-73	35.657774542237526	37.0	35.0	39.5	31.0	41.0
74-75	35.269518776406386	36.5	35.0	39.0	31.0	41.0
76-77	34.89101391713944	36.0	35.0	39.0	30.5	40.5
78-79	34.514045076220796	35.5	35.0	37.0	30.0	39.0
80-81	34.17922544895711	35.0	35.0	37.0	30.0	39.0
82-83	33.87386857525557	35.0	35.0	36.5	29.5	39.0
84-85	33.5827696604392	35.0	34.5	36.0	29.0	37.0
86-87	33.3590115925229	35.0	34.0	36.0	29.0	37.0
88-89	33.182519173683765	35.0	34.0	35.5	29.0	37.0
90-91	32.98701027275397	35.0	34.0	35.0	29.0	36.0
92-93	32.86028448582621	35.0	34.0	35.0	29.0	36.0
94-95	32.74967562586727	35.0	34.0	35.0	28.5	36.0
96-97	32.634049002377964	35.0	34.0	35.0	27.0	36.0
98-99	32.562594495817706	35.0	34.0	35.0	27.0	35.5
100-101	31.370060513580725	34.5	31.5	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08097855936333787
1101	2	0.004971759851883917
1101	3	0.05067860551628911
1101	4	-0.11442474159880334
1101	5	-0.06974371251358491
1101	6	-0.08913228062361611
1101	7	-0.004774638865526981
1101	8	0.03078822740663867
1101	9	0.1588480890615287
1101	10-11	0.2901561634879428
1101	12-13	0.4125323512887604
1101	14-15	0.43371279049036104
1101	16-17	0.3622454782918041
1101	18-19	0.36678358321852755
1101	20-21	0.37479372322922444
1101	22-23	0.4007107752112873
1101	24-25	0.4216376993365216
1101	26-27	0.40401138920083923
1101	28-29	0.37666646384935376
1101	30-31	0.3323014049041504
1101	32-33	0.3767749400314173
1101	34-35	0.41129269945468394
1101	36-37	0.35038765657644433
1101	38-39	0.13381944191876016
1101	40-41	0.1641793114902299
1101	42-43	0.22199912934640764
1101	44-45	0.2016433460901581
1101	46-47	0.31526065304193907
1101	48-49	0.22105033739489244
1101	50-51	0.3099121229514239
1101	52-53	0.34929418583324434
1101	54-55	0.44375505075552724
1101	56-57	0.36782378666599413
1101	58-59	0.33546543586903965
1101	60-61	0.45706795605370587
1101	62-63	0.4390272880888233
1101	64-65	0.316100563795807
1101	66-67	0.2840108814126552
1101	68-69	0.22715359860021067
1101	70-71	0.2444731674414058
1101	72-73	0.15133938650227918
1101	74-75	0.1362831663349482
1101	76-77	0.242493899470972
1101	78-79	0.19151584615126183
1101	80-81	0.17456907773001973
1101	82-83	0.17898082387436176
1101	84-85	0.1676451629504001
1101	86-87	0.2101739329967245
1101	88-89	0.16611042899901207
1101	90-91	0.16422462540713667
1101	92-93	0.12272635359478556
1101	94-95	0.1170148330684313
1101	96-97	0.19097138133367508
1101	98-99	0.06937013817128701
1101	100-101	-0.03098596827699751
1102	1	-0.09302370754012657
1102	2	0.0036334570075098327
1102	3	0.11496109010277422
1102	4	0.00973443488222614
1102	5	-0.049056009940507295
1102	6	-0.18281939581400763
1102	7	0.0064604283031641785
1102	8	-0.03340298044653878
1102	9	0.14816659837725155
1102	10-11	0.22344956325045473
1102	12-13	0.30759791418307714
1102	14-15	0.35835095853251175
1102	16-17	0.35452055086948775
1102	18-19	0.26773184705899666
1102	20-21	0.2353816130041153
1102	22-23	0.24788246421117321
1102	24-25	0.32085529715264016
1102	26-27	0.3197663449781132
1102	28-29	0.22558382037373548
1102	30-31	0.29345808395233774
1102	32-33	0.33621088362577467
1102	34-35	0.3560694306489225
1102	36-37	0.32814275603004717
1102	38-39	0.37648772283136367
1102	40-41	0.18862605193892534
1102	42-43	0.38833195981255386
1102	44-45	0.29813975629456024
1102	46-47	0.3643736629344829
1102	48-49	0.4319044509752743
1102	50-51	0.3460304530942935
1102	52-53	0.4390177356530174
1102	54-55	0.4418090815107121
1102	56-57	0.2256742195966055
1102	58-59	0.26470420481635415
1102	60-61	0.1883234759718917
1102	62-63	0.15357472874838862
1102	64-65	0.027070674891419344
1102	66-67	0.17857040778783073
1102	68-69	0.12245538229971231
1102	70-71	0.12115449754625018
1102	72-73	0.11276778286303824
1102	74-75	0.017255348060160713
1102	76-77	0.1304181144276768
1102	78-79	0.11927052302739583
1102	80-81	0.0481252361627611
1102	82-83	0.04517906341662581
1102	84-85	0.11101238699674809
1102	86-87	-0.059989139648571665
1102	88-89	0.002051065316159395
1102	90-91	0.07916654213402552
1102	92-93	0.024747541629082548
1102	94-95	0.05116205060709689
1102	96-97	0.15471761511198423
1102	98-99	0.05573229551378489
1102	100-101	-0.06274604569319209
1103	1	-0.009622803749969933
1103	2	0.0031013648728759335
1103	3	0.09101250658125082
1103	4	-0.08555402844053361
1103	5	-0.005007333393628244
1103	6	-0.044010717427596546
1103	7	0.05587157698479217
1103	8	0.14698230428124504
1103	9	0.21355745413791993
1103	10-11	0.19033459342923464
1103	12-13	0.28373525607829464
1103	14-15	0.4181475855766692
1103	16-17	0.40012939900902467
1103	18-19	0.37217640983578093
1103	20-21	0.3812377891859171
1103	22-23	0.2864220306468539
1103	24-25	0.4122744945850414
1103	26-27	0.40370256814860994
1103	28-29	0.38837920412149174
1103	30-31	0.4353665318327202
1103	32-33	0.4351683219026867
1103	34-35	0.31861643749956414
1103	36-37	0.37951728031386267
1103	38-39	0.3768304159840312
1103	40-41	0.3168342367181012
1103	42-43	0.25796898342066044
1103	44-45	0.41053302522820445
1103	46-47	0.3528525974915837
1103	48-49	0.47679846419245564
1103	50-51	0.29479611229553626
1103	52-53	0.30788284243046604
1103	54-55	0.46006641724189024
1103	56-57	0.5281998620807187
1103	58-59	0.45429540960364534
1103	60-61	0.4782362354525276
1103	62-63	0.5285828977140596
1103	64-65	0.4079913596806861
1103	66-67	0.4270043868123423
1103	68-69	0.5147726818695944
1103	70-71	0.48381260233008305
1103	72-73	0.4862960579728295
1103	74-75	0.3452838962756175
1103	76-77	0.448469430861536
1103	78-79	0.3717837406155695
1103	80-81	0.3797685012640528
1103	82-83	0.40998832916175587
1103	84-85	0.4033816855629624
1103	86-87	0.3326367652912907
1103	88-89	0.30523587273430763
1103	90-91	0.3962297162753927
1103	92-93	0.41489290484348373
1103	94-95	0.2950897861214017
1103	96-97	0.3138615720540727
1103	98-99	0.32711905105879424
1103	100-101	0.18847849234675706
1104	1	0.3459302018003143
1104	2	0.34059107258187993
1104	3	0.3901043691789141
1104	4	0.22408156909616395
1104	5	0.27520509546625505
1104	6	0.24754145064727595
1104	7	0.28812806993570206
1104	8	0.29298205062282534
1104	9	0.4457595917133901
1104	10-11	0.4512154323541253
1104	12-13	0.437141425614719
1104	14-15	0.5452312586311052
1104	16-17	0.45961048635810897
1104	18-19	0.4997257272731943
1104	20-21	0.4881974917210883
1104	22-23	0.43820800736261845
1104	24-25	0.5357753431328476
1104	26-27	0.546951529860273
1104	28-29	0.531719764706601
1104	30-31	0.5303686557186893
1104	32-33	0.5371983945174463
1104	34-35	0.5166364926199449
1104	36-37	0.5778320623588726
1104	38-39	0.6090406062077136
1104	40-41	0.5585276281110296
1104	42-43	0.5600467679760897
1104	44-45	0.6048772477550841
1104	46-47	0.5474573231881905
1104	48-49	0.5964728956251619
1104	50-51	0.5423006972721041
1104	52-53	0.47853637522790393
1104	54-55	0.5449381083584086
1104	56-57	0.6694908223791529
1104	58-59	0.634330881519034
1104	60-61	0.6514742656942474
1104	62-63	0.70164920523262
1104	64-65	0.6094492916398337
1104	66-67	0.563728086359987
1104	68-69	0.5581313609131868
1104	70-71	0.4805184652619303
1104	72-73	0.43034907660867106
1104	74-75	0.5159708475631319
1104	76-77	0.5197406017769879
1104	78-79	0.5406306617861816
1104	80-81	0.5348333150163711
1104	82-83	0.5216099110642247
1104	84-85	0.5009668976797812
1104	86-87	0.5150854145602111
1104	88-89	0.4476475603479244
1104	90-91	0.45342948838545283
1104	92-93	0.4888109812268766
1104	94-95	0.41952856686992845
1104	96-97	0.4972398995592968
1104	98-99	0.4995296716965214
1104	100-101	0.4723997696578195
1105	1	0.21265777642800998
1105	2	0.23349893971604274
1105	3	0.2549602285741841
1105	4	0.23448867642512994
1105	5	0.2017015410605012
1105	6	0.18887151743600583
1105	7	0.2128017243392364
1105	8	0.19675883838885255
1105	9	0.29467696197202287
1105	10-11	0.2826105829438106
1105	12-13	0.2880051943967388
1105	14-15	0.36835917276199837
1105	16-17	0.3166147783308588
1105	18-19	0.366805406746586
1105	20-21	0.25015534291787134
1105	22-23	0.315696647348382
1105	24-25	0.3167329571221629
1105	26-27	0.34408187536779167
1105	28-29	0.33959116119147836
1105	30-31	0.43430055493515596
1105	32-33	0.321393334585494
1105	34-35	0.32040957401916614
1105	36-37	0.26360869445748847
1105	38-39	0.22773144332771977
1105	40-41	0.2564231530437553
1105	42-43	0.2001756012859488
1105	44-45	0.14088070950167264
1105	46-47	0.17870088337588186
1105	48-49	0.09674160721793612
1105	50-51	0.10171394234449593
1105	52-53	0.06820580437328516
1105	54-55	0.10389380244971846
1105	56-57	0.19924794416295555
1105	58-59	0.21664762021275408
1105	60-61	0.18331955453141546
1105	62-63	0.20187751742730597
1105	64-65	0.27058395877103436
1105	66-67	0.3248438510205247
1105	68-69	0.3277784406667834
1105	70-71	0.3017513140495751
1105	72-73	0.04710370771397976
1105	74-75	0.11641246116697346
1105	76-77	0.0028910876816681252
1105	78-79	0.18025969153347887
1105	80-81	0.16860072567439488
1105	82-83	0.10995338748802652
1105	84-85	0.16759921868079175
1105	86-87	0.13986618296417674
1105	88-89	0.11217855669345056
1105	90-91	0.13221850716190175
1105	92-93	0.03460907318925166
1105	94-95	0.025439480637601264
1105	96-97	0.15330129181892005
1105	98-99	0.19657699458778666
1105	100-101	-0.030453206184080983
1106	1	0.05032132484647178
1106	2	0.08462798063343513
1106	3	0.20463621516093156
1106	4	0.10891534100232292
1106	5	0.09652233247740583
1106	6	0.1344715807006054
1106	7	0.19448727606893357
1106	8	0.23829291783815876
1106	9	0.2743125811017251
1106	10-11	0.2517553065166922
1106	12-13	0.2845036947645738
1106	14-15	0.36758668444635845
1106	16-17	0.35217173170514116
1106	18-19	0.2633761317792036
1106	20-21	0.2423241723341718
1106	22-23	0.3783391196592376
1106	24-25	0.365872028960851
1106	26-27	0.34314723524322943
1106	28-29	0.2742187706747856
1106	30-31	0.3355274310714904
1106	32-33	0.3823192468073131
1106	34-35	0.31242459500821695
1106	36-37	0.43638942748523846
1106	38-39	0.35261336633934803
1106	40-41	0.3396893765680318
1106	42-43	0.3811703935647657
1106	44-45	0.288161528865281
1106	46-47	0.28608709778773544
1106	48-49	0.26395277022729857
1106	50-51	0.24930927092137978
1106	52-53	0.34475607723813795
1106	54-55	0.2426609263336985
1106	56-57	0.34193446227389046
1106	58-59	0.4768644538196085
1106	60-61	0.45124748657710967
1106	62-63	0.436304051412435
1106	64-65	0.4299545195478558
1106	66-67	0.37516314860936006
1106	68-69	0.30096643954659186
1106	70-71	0.35327260273202654
1106	72-73	0.41411129221067
1106	74-75	0.4384813223606798
1106	76-77	0.2970261256767017
1106	78-79	0.33343746795696916
1106	80-81	0.3549485228039799
1106	82-83	0.23555392959018207
1106	84-85	0.37905823671556504
1106	86-87	0.36781825277579827
1106	88-89	0.3523252043227032
1106	90-91	0.26182151455196134
1106	92-93	0.2509500577663317
1106	94-95	0.2600974527750637
1106	96-97	0.35499964157057917
1106	98-99	0.36208774013785927
1106	100-101	0.3395092050677313
1107	1	0.23928588858225197
1107	2	0.2428312349957764
1107	3	0.22157036206820635
1107	4	0.2635369928396756
1107	5	0.29661645729797215
1107	6	0.26283746664382335
1107	7	0.3039103754581518
1107	8	0.20666191908661347
1107	9	0.20659696187323817
1107	10-11	0.23370084791913115
1107	12-13	0.24891133245457553
1107	14-15	0.2563909231253376
1107	16-17	0.1920480381113876
1107	18-19	0.22689876983032065
1107	20-21	0.26965897878086054
1107	22-23	0.2339483831658029
1107	24-25	0.24677500817117703
1107	26-27	0.22365248050529374
1107	28-29	0.2813887987757724
1107	30-31	0.3208696869585381
1107	32-33	0.25778425437424346
1107	34-35	0.3375528933293097
1107	36-37	0.3018190583648348
1107	38-39	0.17968946266218921
1107	40-41	0.16317305333380716
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2316	88-89	-0.30152556121871754
2316	90-91	-0.26114517776022694
2316	92-93	-0.20429242742000753
2316	94-95	-0.2893412701677889
2316	96-97	-0.34519809892371
2316	98-99	-0.30598898047438894
2316	100-101	-0.13134330616906098
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3063.0
3	173.0
4	86.0
5	52.0
6	318.0
7	2090.0
8	2184.0
9	1285.0
10	1092.0
11	1244.0
12	1578.0
13	1985.0
14	2084.0
15	2293.0
16	2805.0
17	3737.0
18	4780.0
19	6151.0
20	8069.0
21	10495.0
22	11723.0
23	11748.0
24	10417.0
25	9176.0
26	8935.0
27	9651.0
28	10868.0
29	12483.0
30	14888.0
31	18439.0
32	23637.0
33	32411.0
34	50285.0
35	86190.0
36	173041.0
37	423591.0
38	714841.0
39	252610.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98865557237558	20.116482266704402	14.24698083469437	22.647881326225647
2	14.28046168027732	21.75833192682705	41.868636641003334	22.09256975189229
3	16.930685458572075	29.38451246722704	30.922192536699477	22.7626095375014
4	11.255343581028969	18.637340744276244	41.25491251095204	28.852403163742746
5	12.278420336226189	40.17591866008084	34.935543058109666	12.610117945583314
6	27.090281379987864	39.18965720460189	21.06681496089363	12.653246454516614
7	25.99751588143111	33.88713668987047	24.1077891448588	16.007558283839625
8	25.996483579479307	34.6768289073445	22.17286955907659	17.1538179540996
9	28.259539876090127	15.230830420433705	20.949511816714747	35.56011788676142
10-11	24.134146088184593	27.0682153315749	29.54298116264097	19.254657417599535
12-13	25.565969988363594	24.853168959416944	29.367012954779	20.213848097440458
14-15	22.968037881183566	25.485990058214437	27.77676521735518	23.769206843246817
16-17	21.616766218445786	28.58008836398947	27.738798413745464	22.06434700381928
18-19	21.589723736147626	27.703450988776947	29.700569715702628	21.006255559372804
20-21	23.90609841877471	26.48242951841943	28.9239972466136	20.687474816192257
22-23	22.9755755124497	26.814409967547338	28.576118423016084	21.63389609698687
24-25	22.137770105975207	26.967943232813514	29.065309204008962	21.82897745720232
26-27	22.13618534549321	27.184642404526965	28.683397011003635	21.995775238976183
28-29	22.181705589885112	27.196067077894625	28.716331868073112	21.90589546414715
30-31	22.461486075085997	27.002337386082925	28.644009818786397	21.89216672004468
32-33	22.18225852203054	27.14000943107568	28.609508050282116	22.068223996611664
34-35	22.51583969658778	27.400255386482886	28.454159268061613	21.62974564886772
36-37	22.590344255065244	26.938231250162143	28.57420810958051	21.897216385192102
38-39	22.414863235749735	27.306273349978127	28.280310276092163	21.998553138179975
40-41	22.515243241953016	27.20261959857625	28.269160069542547	22.01297708992819
42-43	22.303769096470088	27.080422437655166	28.213589915870468	22.402218550004278
44-45	22.61631573063406	27.20293126716972	28.067516051442432	22.113236950753787
46-47	22.522977534662477	27.135864668929205	28.01382426436999	22.327333532038327
48-49	22.49176884915385	27.309054421578118	27.74209129858662	22.457085430681413
50-51	22.70124968837561	27.42203812855709	27.46328539398013	22.41342678908717
52-53	22.908344930577393	27.32225083048721	27.389499279258523	22.379904959676875
54-55	22.645103385894867	27.359216846883943	27.534335471653954	22.461344295567233
56-57	22.72170946744165	27.486657434745393	27.327472957450393	22.464160140362566
58-59	22.817308121472813	27.25161380057691	27.45036116540327	22.48071691254701
60-61	22.848113273410224	27.235288209415454	27.542758994240664	22.373839522933654
62-63	22.550719793817482	27.254569861013117	27.78707482445968	22.407635520709725
64-65	22.45217967576864	27.301171061791923	27.63421438233373	22.612434880105702
66-67	22.505944866712017	27.40532965148527	27.520776550322136	22.56794893148058
68-69	22.75367942886818	27.317473402640978	27.46989558198762	22.45895158650322
70-71	22.51148378312889	27.055523241786496	27.62770179574043	22.805291179344188
72-73	22.50598646268157	27.07894347689602	27.560921824450713	22.8541482359717
74-75	22.464293982743172	27.26053323615856	27.47803445343865	22.797138327659614
76-77	22.494924784027564	27.095320627515225	27.634349829926414	22.7754047585308
78-79	22.586680760001787	27.195040405854826	27.41422776172434	22.804051072419046
80-81	22.45785840632166	27.141665055278196	27.412066855534373	22.988409682865772
82-83	22.581505848996713	27.216398733104537	27.28146689115657	22.92062852674218
84-85	22.684014005483025	27.182232178355033	27.256064077264757	22.87768973889719
86-87	22.44975959854384	27.238899109230225	27.44821573598134	22.863125556244597
88-89	22.49275687192344	27.084200861210327	27.48862776353843	22.934414503327805
90-91	22.531837922445536	27.072519438421956	27.487876764529336	22.90776587460318
92-93	22.565427139276206	27.060571936713885	27.54457465841854	22.829426265591373
94-95	22.489638439450534	27.07938756996664	27.521996185088327	22.908977805494498
96-97	22.481245866540466	26.896461719721543	27.65007782384853	22.972214589889465
98-99	22.323789223422352	26.96570154954392	27.4264630149418	23.284046212091926
100-101	22.61260527185791	26.847223656669595	27.406727330383013	23.133443741089486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2886.0
1	1507.0
2	129.0
3	153.0
4	143.5
5	128.5
6	173.5
7	277.0
8	437.5
9	559.5
10	543.5
11	489.5
12	519.0
13	617.5
14	699.0
15	748.5
16	842.0
17	930.0
18	1010.5
19	1141.0
20	1280.0
21	1485.0
22	1797.5
23	2251.0
24	2902.0
25	3959.0
26	6268.0
27	9321.5
28	12918.5
29	18576.5
30	25104.0
31	31813.0
32	39268.0
33	45802.0
34	51284.0
35	54643.0
36	59521.0
37	63089.0
38	61323.5
39	61355.0
40	63082.0
41	64705.5
42	65213.5
43	65588.0
44	69071.0
45	72446.0
46	73264.0
47	73746.0
48	74213.5
49	74102.0
50	71894.0
51	67964.5
52	64126.5
53	61270.5
54	58653.5
55	54936.5
56	49726.5
57	43476.5
58	38378.5
59	34173.0
60	29363.0
61	23622.5
62	18236.0
63	13256.0
64	9143.5
65	6997.5
66	5515.0
67	4041.0
68	3033.5
69	2302.5
70	1787.0
71	1492.0
72	1207.0
73	994.0
74	811.0
75	625.5
76	428.5
77	311.0
78	249.0
79	197.5
80	146.5
81	95.5
82	69.0
83	55.0
84	47.5
85	36.5
86	21.5
87	12.5
88	11.5
89	8.0
90	7.0
91	8.5
92	6.5
93	5.0
94	4.0
95	2.0
96	1.5
97	2.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2603280464881871
2	0.2623998972362029
3	0.2731217248571847
4	0.2693405972420559
5	0.26374660022241314
6	0.24551431363987403
7	0.2489846636428005
8	0.24960621886720527
9	0.24473736960936807
10-11	0.24245833378655068
12-13	0.248000534537493
14-15	0.25017597782290957
16-17	0.1987940792721174
18-19	0.19242313822196877
20-21	0.18436881843905728
22-23	0.2065894177115269
24-25	0.21264958114947316
26-27	0.2145142468226874
28-29	0.2141516729417846
30-31	0.21391858973263284
32-33	0.2088166572656439
34-35	0.19462447964173557
36-37	0.17046152029300113
38-39	0.19226774941586758
40-41	0.18001793186822407
42-43	0.1686486508884873
44-45	0.15593266692254024
46-47	0.16370210722759954
48-49	0.16611063372216792
50-51	0.16784580872363117
52-53	0.17916329343466755
54-55	0.18965203784649762
56-57	0.18809814978548575
58-59	0.19801713524161146
60-61	0.2132193401051775
62-63	0.19705890427065414
64-65	0.19239724008761858
66-67	0.17341390760892367
68-69	0.1605684329712256
70-71	0.17393187029592763
72-73	0.17486420313253473
74-75	0.21832127257216646
76-77	0.21350421958302968
78-79	0.2297164516862534
80-81	0.22005644757362972
82-83	0.2175184304073103
84-85	0.20547579793446838
86-87	0.18936715836864543
88-89	0.1797071542560217
90-91	0.16284746879404302
92-93	0.16432366245200428
94-95	0.16598114305041695
96-97	0.1829703191841466
98-99	0.17592602664089285
100-101	0.19910485688431978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1930641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.17075591987838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.47620851729687	19.065292839272896
2	16.039481873671015	10.640834766257097
3	7.741619366646551	7.703860993069088
4	4.6152436870403175	6.123644874142958
5	3.0107760143076936	4.993485815001239
6	2.1196099997743834	4.218543956870973
7	1.547440000712244	3.593082819499759
8	1.1671319279193535	3.097171864584797
9	0.9151812859489773	2.7321529552788557
>10	5.103351271215988	29.34160605793492
>50	0.20132084901966768	4.48327393744149
>100	0.06121548283077059	3.3746906789329145
>500	9.464824108173068E-4	0.1915127758932416
>1k	4.732412054086534E-4	0.4408456658198252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3709	0.19211236060976639	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2854	0.14782655087092836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.589813435019768E-5	0.0
14-15	0.0	0.0	0.0	5.179626870039536E-5	0.0
16-17	0.0	0.0	0.0	1.5538880610118608E-4	0.0
18-19	0.0	0.0	0.0	4.920645526537559E-4	0.0
20-21	0.0	0.0	0.0	8.028421648561281E-4	0.0
22-23	0.0	0.0	0.0	0.0017610731358134422	0.0
24-25	0.0	0.0	0.0	0.003392655599875896	0.0
26-27	0.0	0.0	0.0	0.004532173511284594	0.0
28-29	0.0	0.0	0.0	0.005542200750942304	0.0
30-31	0.0	0.0	0.0	0.008287402992063257	0.0
32-33	0.0	0.0	0.0	0.01261239142854627	0.0
34-35	0.0	0.0	0.0	0.020045155987053004	0.0
36-37	0.0	0.0	0.0	0.03224317726599611	0.0
38-39	0.0	0.0	0.0	0.055629192584224614	0.0
40-41	0.0	0.0	0.0	0.0882090455967733	0.0
42-43	0.0	0.0	0.0	0.1217989258489797	0.0
44-45	0.0	0.0	0.0	0.1587037672980114	0.0
46-47	0.0	0.0	0.0	0.20560528860621938	0.0
48-49	0.0	0.0	0.0	0.26079421290649063	0.0
50-51	0.0	0.0	0.0	0.3298127409497674	0.0
52-53	0.0	0.0	0.0	0.39813201936558895	0.0
54-55	0.0	0.0	0.0	0.466917464199714	0.0
56-57	0.0	0.0	0.0	0.5435500437419489	0.0
58-59	0.0	0.0	0.0	0.6373012900896645	0.0
60-61	0.0	0.0	0.0	0.7495438043634213	0.0
62-63	0.0	0.0	0.0	0.8719124891681054	0.0
64-65	0.0	0.0	0.0	0.9904741482233104	0.0
66-67	0.0	0.0	0.0	1.1148369893729595	0.0
68-69	0.0	0.0	0.0	1.2742918025671268	0.0
70-71	0.0	0.0	0.0	1.4596965463801919	0.0
72-73	0.0	0.0	0.0	1.6655349181955632	0.0
74-75	0.0	0.0	0.0	1.8595119444785437	0.0
76-77	0.0	0.0	0.0	2.066619324877075	0.0
78-79	0.0	0.0	0.0	2.3089740661262246	0.0
80-81	0.0	0.0	0.0	2.5903572958411223	0.0
82-83	0.0	0.0	0.0	2.90188595393965	0.0
84-85	0.0	0.0	0.0	3.203832302328605	0.0
86-87	0.0	0.0	0.0	3.514454525724876	0.0
88-89	0.0	0.0	0.0	3.861644914823626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3515	0.0	59.484764	1
GTATCAA	6825	0.0	44.004066	1
ATCAACG	9150	0.0	32.666904	3
TATCAAC	9320	0.0	32.122036	2
TCAACGC	9295	0.0	32.106182	4
CAACGCA	9385	0.0	31.69702	5
AACGCAG	9540	0.0	31.37883	6
ACGCAGA	10765	0.0	27.852226	7
CGCAGAG	10845	0.0	27.427698	8
GTACATG	13770	0.0	27.297379	1
TACATGG	13860	0.0	26.674406	2
ACATGGG	14150	0.0	25.187393	3
GCAGAGT	12145	0.0	23.904972	9
CATGGGG	7465	0.0	23.744194	4
AGAGTAC	11540	0.0	21.740656	10-11
CATGGGA	6655	0.0	20.493317	4
ATGGGGG	3250	0.0	19.739168	5
CAGAGTA	12075	0.0	19.636223	10-11
GTATAAT	1125	0.0	19.008085	1
ATGGGAT	2655	0.0	18.793304	5
>>END_MODULE