FastQCFastQC Report
Fri 10 Feb 2017
SRR2568574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930641
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.23240985765867397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.187606085232832No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT29870.15471545460808095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.12317152696954016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.060.2137641
GTATCAA69550.045.7021031
ATCAACG93000.033.9166953
TCAACGC93750.033.6427464
CAACGCA95100.033.165175
AACGCAG96550.032.8596236
TATCAAC96350.032.8386042
GCCCGTA753.081513E-431.6626457
ACGCAGA109750.028.7343887
CGCAGAG110500.028.45348
GTACATG133850.027.6164861
TACATGG133050.027.6011262
ACATGGG135400.026.2077883
CATTCCG2101.2678356E-924.877799
GCAGAGT126750.024.430829
CATGGGG69650.023.1874224
CATGGGA63400.022.4763954
AGAGTAC121150.021.07140410-11
GAGTACT53000.020.87942312-13
ATTATAC8800.020.5130063
Produced by FastQC (version 0.11.5)