FastQCFastQC Report
Fri 10 Feb 2017
SRR2568573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477570
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.132650229769148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.054.3089641
GTATCAA30200.041.5409661
TCAACGC35500.034.5347444
ATCAACG36850.033.270693
CAACGCA37300.032.6133965
AACGCAG39100.031.5981376
TATCAAC40400.030.5824012
TATACCG1601.899025E-929.6992115
GTATACG856.392019E-427.9531441
ACGCAGA44350.027.6433667
GTACATG51150.027.2208441
TACATGG50650.026.4575272
CGCAGAG46500.026.2621528
ACATGGG51650.025.2092463
TACCGTA1252.2503073E-422.8089947
GCAGAGT55450.021.680499
AGAGTAC49350.020.7977710-11
CATGGGG35000.020.7724764
GTACCGT1456.1308657E-419.6629266
GTGGTAT9450.019.1087111
Produced by FastQC (version 0.11.5)