##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568573_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477570 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.987067279384394 34.0 31.0 34.0 30.0 34.0 2 32.12598523251013 34.0 31.0 34.0 30.0 34.0 3 32.25982863756032 34.0 31.0 34.0 30.0 34.0 4 35.43700806053182 37.0 35.0 37.0 33.0 37.0 5 35.482904363245055 37.0 35.0 37.0 33.0 37.0 6 35.613298185534354 37.0 37.0 37.0 35.0 37.0 7 35.651609737609725 37.0 37.0 37.0 35.0 37.0 8 35.684984806134395 37.0 37.0 37.0 35.0 37.0 9 37.446907422321786 39.0 38.0 39.0 35.0 39.0 10-11 37.45452871945153 39.0 38.5 39.0 35.0 39.0 12-13 37.437198237646946 39.0 38.0 39.0 35.0 39.0 14-15 38.91057851743065 41.0 39.5 41.0 36.0 41.0 16-17 38.90458692312377 41.0 39.0 41.0 36.0 41.0 18-19 38.931095650290686 41.0 39.5 41.0 36.0 41.0 20-21 38.927716453366 41.0 39.5 41.0 36.0 41.0 22-23 38.85459199902543 41.0 39.0 41.0 36.0 41.0 24-25 38.79368490156135 41.0 39.0 41.0 35.0 41.0 26-27 38.71302341005841 41.0 39.0 41.0 35.0 41.0 28-29 38.62620180431384 41.0 39.0 41.0 35.0 41.0 30-31 38.51903023207022 40.5 39.0 41.0 34.5 41.0 32-33 38.392819629526855 40.0 38.5 41.0 34.0 41.0 34-35 38.28994463883268 40.0 38.0 41.0 34.0 41.0 36-37 38.17846396448222 40.0 38.0 41.0 34.0 41.0 38-39 38.06914562423439 40.0 38.0 41.0 33.0 41.0 40-41 37.93828955650156 40.0 38.0 41.0 33.0 41.0 42-43 37.81598773662162 40.0 38.0 41.0 33.0 41.0 44-45 37.66796632308453 40.0 38.0 41.0 32.0 41.0 46-47 37.6175314875099 40.0 38.0 41.0 32.5 41.0 48-49 37.74408488261131 40.0 38.0 41.0 32.5 41.0 50-51 36.28957477479916 38.5 36.0 40.0 30.0 40.5 52-53 36.51339124373126 39.0 36.5 39.5 30.5 40.5 54-55 37.34641675182901 40.0 37.0 41.0 31.5 41.0 56-57 37.352464857840914 40.0 37.0 41.0 31.5 41.0 58-59 37.17815298090784 40.0 36.5 41.0 31.0 41.0 60-61 36.95743315037528 40.0 36.0 41.0 31.0 41.0 62-63 36.69952388042529 39.0 35.5 41.0 30.5 41.0 64-65 36.4256519149685 39.0 35.0 41.0 30.0 41.0 66-67 36.33389653282078 39.0 35.0 41.0 30.0 41.0 68-69 36.04221221329615 38.0 35.0 40.5 30.0 41.0 70-71 35.69547567966323 37.0 35.0 40.0 30.0 41.0 72-73 35.35475375109132 37.0 35.0 39.0 29.0 41.0 74-75 34.984260982559206 36.0 35.0 39.0 29.0 41.0 76-77 34.61924071279195 36.0 35.0 38.5 29.0 40.5 78-79 34.262591620024764 35.0 34.5 37.0 29.0 39.0 80-81 33.93019721569875 35.0 34.0 37.0 29.0 39.0 82-83 33.62094317020514 35.0 34.0 36.5 28.5 39.0 84-85 33.338605277584136 35.0 34.0 36.0 28.0 37.0 86-87 33.12086026381153 35.0 34.0 36.0 27.0 37.0 88-89 32.950630426984844 35.0 34.0 35.0 27.0 36.5 90-91 32.7571597284731 35.0 34.0 35.0 27.0 36.0 92-93 32.62868459700725 35.0 34.0 35.0 26.0 36.0 94-95 32.512760139959525 35.0 34.0 35.0 26.0 36.0 96-97 32.40069167619808 35.0 34.0 35.0 26.0 36.0 98-99 32.332064809112254 35.0 34.0 35.0 25.0 35.0 100-101 31.10658682837361 34.5 31.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.034638342158139324 1101 2 0.04302127134527467 1101 3 0.13858682374139164 1101 4 -0.02174174113152816 1101 5 0.06322551331962956 1101 6 -0.0808462502642513 1101 7 0.04736465263194134 1101 8 0.14158452396313237 1101 9 0.26388690323386754 1101 10-11 0.26165768621397234 1101 12-13 0.41449554886728635 1101 14-15 0.39935332890105 1101 16-17 0.4278916017401926 1101 18-19 0.40584293372401703 1101 20-21 0.4578175566682958 1101 22-23 0.4424753291438819 1101 24-25 0.43200537600868216 1101 26-27 0.4736642894329677 1101 28-29 0.4006690972822682 1101 30-31 0.4179856027151203 1101 32-33 0.5251426773985131 1101 34-35 0.5110654471071356 1101 36-37 0.41712002074549304 1101 38-39 0.4515248941017873 1101 40-41 0.48198518051437134 1101 42-43 0.44240186756977096 1101 44-45 0.6508435443419671 1101 46-47 0.5649676477074266 1101 48-49 0.5934274815752687 1101 50-51 0.5003527663658858 1101 52-53 0.5523200618824333 1101 54-55 0.6009778113961559 1101 56-57 0.4786251090282079 1101 58-59 0.4860419896539554 1101 60-61 0.4966637609644309 1101 62-63 0.6919618539798691 1101 64-65 0.6414018831138222 1101 66-67 0.679153629523455 1101 68-69 0.6635104741825373 1101 70-71 0.6080471438624429 1101 72-73 0.4862250212815553 1101 74-75 0.4327880399654589 1101 76-77 0.46701623116826596 1101 78-79 0.4475803969363952 1101 80-81 0.5522258950343755 1101 82-83 0.41997202366253106 1101 84-85 0.3451090436510782 1101 86-87 0.375209538190326 1101 88-89 0.44183570908580094 1101 90-91 0.496059154649096 1101 92-93 0.483668281801819 1101 94-95 0.31609432786315494 1101 96-97 0.40371900237105507 1101 98-99 0.3475665098577352 1101 100-101 0.22864774309193692 1102 1 0.023157314817623842 1102 2 0.1351816690254708 1102 3 0.28102460336027946 1102 4 0.1936975297881105 1102 5 0.10630422085898061 1102 6 -0.027654535268396785 1102 7 0.2165987868490049 1102 8 0.07565908900041762 1102 9 0.21486751632417622 1102 10-11 0.38518179557603105 1102 12-13 0.43873084298493126 1102 14-15 0.6048008848248188 1102 16-17 0.5340689008288351 1102 18-19 0.48217797929154216 1102 20-21 0.5837708292449264 1102 22-23 0.552077648945037 1102 24-25 0.5082297339208637 1102 26-27 0.5107864103940329 1102 28-29 0.4646343002648692 1102 30-31 0.3499713525079926 1102 32-33 0.4671131827837698 1102 34-35 0.4899970129729141 1102 36-37 0.35454769265932384 1102 38-39 0.431988854333774 1102 40-41 0.43374093859225127 1102 42-43 0.3795147093262017 1102 44-45 0.28746160565099643 1102 46-47 0.423859776953492 1102 48-49 0.4138897848064218 1102 50-51 0.25809659403780216 1102 52-53 0.4649172449810308 1102 54-55 0.41280780311115706 1102 56-57 0.34321566412348403 1102 58-59 0.46007612387102625 1102 60-61 0.43900874853692784 1102 62-63 0.35841587220687643 1102 64-65 0.5133853131055375 1102 66-67 0.4364450959691908 1102 68-69 0.46310384617921585 1102 70-71 0.4451497122137269 1102 72-73 0.3586150792765821 1102 74-75 0.44264255529271423 1102 76-77 0.4214139113671038 1102 78-79 0.28458951044094505 1102 80-81 0.2495713797071133 1102 82-83 0.2259219822375158 1102 84-85 0.22327242393276236 1102 86-87 0.1523127693419397 1102 88-89 0.1722449882904371 1102 90-91 0.14214200469051974 1102 92-93 0.13569645081589243 1102 94-95 0.16755414559306558 1102 96-97 0.0935340810785874 1102 98-99 0.15164140629519096 1102 100-101 -0.05347918317152889 1103 1 0.14299202022521484 1103 2 0.12490305724177375 1103 3 0.15303041449829635 1103 4 0.10443974124896727 1103 5 0.15869163005994835 1103 6 0.08683715157583549 1103 7 0.12370839136877976 1103 8 0.27488749900848575 1103 9 0.26407881575920555 1103 10-11 0.3867579053419732 1103 12-13 0.45672600036991895 1103 14-15 0.4349296983841455 1103 16-17 0.4937848007481307 1103 18-19 0.41303931908169744 1103 20-21 0.3246011762990122 1103 22-23 0.26690412190711044 1103 24-25 0.39480309953830073 1103 26-27 0.340687298206781 1103 28-29 0.3679740904182225 1103 30-31 0.3308078335410869 1103 32-33 0.42251528125026994 1103 34-35 0.3090601283885732 1103 36-37 0.23229555383770162 1103 38-39 0.3683568930746901 1103 40-41 0.2931369030797413 1103 42-43 0.2263989708274039 1103 44-45 0.48330825617561146 1103 46-47 0.256066718035008 1103 48-49 0.3980334490517805 1103 50-51 0.22349320420729413 1103 52-53 0.28494178089708555 1103 54-55 0.3317847199795949 1103 56-57 0.4487833800234 1103 58-59 0.41145756051347604 1103 60-61 0.370920935784703 1103 62-63 0.4011358076386742 1103 64-65 0.3984797440982746 1103 66-67 0.2625697125955071 1103 68-69 0.45189448379019126 1103 70-71 0.3496730374760304 1103 72-73 0.27211015595131727 1103 74-75 0.2858800048488064 1103 76-77 0.36938324147202906 1103 78-79 0.2351221980922773 1103 80-81 0.23585709399283417 1103 82-83 0.24470874575647628 1103 84-85 0.221895184223321 1103 86-87 0.1978840364928658 1103 88-89 0.17296492372224037 1103 90-91 0.13480221453716723 1103 92-93 0.1279918503316324 1103 94-95 0.15292621984649202 1103 96-97 0.07162641360482525 1103 98-99 0.26796360161399235 1103 100-101 0.11542670867673266 1104 1 0.13928477678719986 1104 2 0.08017510377327142 1104 3 0.09894550007765446 1104 4 0.0693369213013213 1104 5 0.09639869643944365 1104 6 0.08013025256187234 1104 7 0.11804058028375408 1104 8 0.16277197931266585 1104 9 0.24747344106396696 1104 10-11 0.3050415233582484 1104 12-13 0.2941639895021453 1104 14-15 0.31744588082161584 1104 16-17 0.3387190209249198 1104 18-19 0.30602521708177477 1104 20-21 0.33283472035782324 1104 22-23 0.28023617576649684 1104 24-25 0.2827376602619367 1104 26-27 0.28284021343646515 1104 28-29 0.3178432674386116 1104 30-31 0.36793164073857554 1104 32-33 0.3041277544410903 1104 34-35 0.3101308800665876 1104 36-37 0.23652815703082553 1104 38-39 0.22106811774450108 1104 40-41 0.18629145900857935 1104 42-43 0.03624297193628934 1104 44-45 0.19860171774067226 1104 46-47 0.09207466886702065 1104 48-49 0.31838458064309094 1104 50-51 0.20813854760064743 1104 52-53 0.27520259325964247 1104 54-55 0.24173366379970673 1104 56-57 0.2585514527543822 1104 58-59 0.3581590702281048 1104 60-61 0.29526751555052755 1104 62-63 0.2940683942244888 1104 64-65 0.3840914780374831 1104 66-67 0.2437504602606282 1104 68-69 0.27139495906953215 1104 70-71 0.2047176865932343 1104 72-73 0.17107016334383474 1104 74-75 0.1840190164616473 1104 76-77 0.3045956567634249 1104 78-79 0.30629487473238015 1104 80-81 0.2353423965205721 1104 82-83 0.15177370361461584 1104 84-85 0.1521095736525453 1104 86-87 0.13735264123945967 1104 88-89 0.16197437179724972 1104 90-91 0.07084176449836832 1104 92-93 0.14915842039156502 1104 94-95 0.2013539854649622 1104 96-97 0.1668186287166975 1104 98-99 0.1380394847579609 1104 100-101 0.09149311466674348 1105 1 0.2571631971705628 1105 2 0.2611757866725313 1105 3 0.28753048571321926 1105 4 0.15539164743516665 1105 5 0.2040395149766283 1105 6 0.3028219353198409 1105 7 0.339328198613714 1105 8 0.3326885007651228 1105 9 0.31580281044182357 1105 10-11 0.36383767792897004 1105 12-13 0.3299367253378733 1105 14-15 0.3645097083542339 1105 16-17 0.3143041949464873 1105 18-19 0.4197720969386012 1105 20-21 0.3441472998331676 1105 22-23 0.39237176659209894 1105 24-25 0.44562385156792317 1105 26-27 0.3328658221587446 1105 28-29 0.3169284179119245 1105 30-31 0.38742429368446096 1105 32-33 0.33370730043082375 1105 34-35 0.33103230709055964 1105 36-37 0.40580063383579557 1105 38-39 0.24368003080436296 1105 40-41 0.2624791738863692 1105 42-43 0.07738529756149148 1105 44-45 0.11647631153335425 1105 46-47 0.2451509534240799 1105 48-49 0.1418015495123015 1105 50-51 0.09650541756720799 1105 52-53 0.2137378332163209 1105 54-55 0.20830486193468545 1105 56-57 0.1803597817705409 1105 58-59 0.1894025944592599 1105 60-61 0.1243822779486905 1105 62-63 0.12988646044217234 1105 64-65 0.23557648957612543 1105 66-67 0.23189803714566892 1105 68-69 0.25750384617921895 1105 70-71 0.2965879475078381 1105 72-73 0.33934743221775676 1105 74-75 0.17557490823389799 1105 76-77 0.22263449960239967 1105 78-79 0.2536336280880036 1105 80-81 0.23443902676594774 1105 82-83 0.2146955116492748 1105 84-85 0.033245953344533064 1105 86-87 0.13861571051840826 1105 88-89 0.11274498829043722 1105 90-91 0.0545978870434638 1105 92-93 0.17610821552177924 1105 94-95 0.15265708676953693 1105 96-97 0.048266434019765825 1105 98-99 0.17757375923635976 1105 100-101 0.04854728741670655 1106 1 0.10505885827822681 1106 2 0.18362943506934215 1106 3 0.2575321581937473 1106 4 0.19109671744042345 1106 5 0.2142912232960228 1106 6 0.21023401062925018 1106 7 0.27355670723893155 1106 8 0.33096046999148143 1106 9 0.3373461515800642 1106 10-11 0.30816408639650206 1106 12-13 0.30776739863074454 1106 14-15 0.4080327288540744 1106 16-17 0.38796979441129764 1106 18-19 0.3747196527277765 1106 20-21 0.32831607701096743 1106 22-23 0.4395106302610401 1106 24-25 0.35782161044401306 1106 26-27 0.3398756061698194 1106 28-29 0.43539140830710465 1106 30-31 0.4147281356111563 1106 32-33 0.36166460439221026 1106 34-35 0.2906442916081673 1106 36-37 0.4368209664204983 1106 38-39 0.27897504442312737 1106 40-41 0.23250324565967873 1106 42-43 0.19252721948053875 1106 44-45 0.295801166983253 1106 46-47 0.4453119296748511 1106 48-49 0.41191253054159915 1106 50-51 0.36893786130018924 1106 52-53 0.4904100150866313 1106 54-55 0.29764907037354504 1106 56-57 0.3512039663168238 1106 58-59 0.5558571149352005 1106 60-61 0.4571864902103542 1106 62-63 0.42134844734903965 1106 64-65 0.4384283675328291 1106 66-67 0.276951675985444 1106 68-69 0.27712204276735264 1106 70-71 0.3974710769578351 1106 72-73 0.4734579712343461 1106 74-75 0.5333044561863005 1106 76-77 0.27582983338172795 1106 78-79 0.346807219620473 1106 80-81 0.3087362862871359 1106 82-83 0.25060695380533815 1106 84-85 0.3920249828279694 1106 86-87 0.4118425825019756 1106 88-89 0.32396554068686356 1106 90-91 0.3387090233745198 1106 92-93 0.24046168233498832 1106 94-95 0.19525845103579798 1106 96-97 0.27112595760174685 1106 98-99 0.4089223161245954 1106 100-101 0.36718499229557366 1107 1 0.5095740395562984 1107 2 0.44674403836345533 1107 3 0.3654204221756103 1107 4 0.42393028240135067 1107 5 0.43509864594609127 1107 6 0.3735357086340443 1107 7 0.3506419924239239 1107 8 0.2932618764568602 1107 9 0.3458166452440352 1107 10-11 0.41139224853770173 1107 12-13 0.3142291008246474 1107 14-15 0.40945872454607724 1107 16-17 0.3023755210378951 1107 18-19 0.2530755402671474 1107 20-21 0.40854574213691564 1107 22-23 0.41030064546071543 1107 24-25 0.40741161545396665 1107 26-27 0.5695933790351475 1107 28-29 0.4575053804042426 1107 30-31 0.5308117706264568 1107 32-33 0.45124419323672527 1107 34-35 0.4605670478161201 1107 36-37 0.46896162994156043 1107 38-39 0.36153589266130126 1107 40-41 0.4462245622856287 1107 42-43 0.4276568696049452 1107 44-45 0.45116195408305515 1107 46-47 0.38129949820784503 1107 48-49 0.30929745031164657 1107 50-51 0.4205049564071359 1107 52-53 0.28123501501587356 1107 54-55 0.3680314964909499 1107 56-57 0.4327181031478702 1107 58-59 0.40116601934140306 1107 60-61 0.34219202379824765 1107 62-63 0.3252799837051299 1107 64-65 0.3919776476003065 1107 66-67 0.3949719252374848 1107 68-69 0.4294550656914069 1107 70-71 0.41513298747504734 1107 72-73 0.32242413851002993 1107 74-75 0.3805157260244272 1107 76-77 0.37549753506048944 1107 78-79 0.4404431689775308 1107 80-81 0.29195465496844264 1107 82-83 0.3343345257915189 1107 84-85 0.4311079640024573 1107 86-87 0.38942914564856324 1107 88-89 0.3734157199977588 1107 90-91 0.42088068064871464 1107 92-93 0.32587763548489335 1107 94-95 0.19580501667320505 1107 96-97 0.3527717117406155 1107 98-99 0.49305464671331123 1107 100-101 0.4283856251908418 1108 1 0.3108297980391015 1108 2 0.329656836810706 1108 3 0.24279104631330028 1108 4 0.33553377139885043 1108 5 0.2776330799193829 1108 6 0.2565280150671754 1108 7 0.2654632163792101 1108 8 0.15890741114806417 1108 9 0.37731583847182293 1108 10-11 0.3370932049719997 1108 12-13 0.3654825208372756 1108 14-15 0.23435524724093426 1108 16-17 0.36499507532548847 1108 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1108 84-85 0.23822275950323757 1108 86-87 0.13952619216073003 1108 88-89 0.10494297486761894 1108 90-91 0.19983327985830357 1108 92-93 0.17203202128569473 1108 94-95 0.2455138771366876 1108 96-97 0.10073139651483132 1108 98-99 0.24916623850995023 1108 100-101 0.07712752823787028 1109 1 0.10695199566868752 1109 2 0.12229124349355658 1109 3 0.01697353953049685 1109 4 0.012118806383853098 1109 5 0.12458081660366105 1109 6 0.17693801792309216 1109 7 0.2453200634447512 1109 8 0.21115376985147805 1109 9 0.23408347377097982 1109 10-11 0.14760254025687658 1109 12-13 0.11642765149557022 1109 14-15 0.14550939546311525 1109 16-17 0.21124177316926307 1109 18-19 0.17018276652558484 1109 20-21 0.19745221222365217 1109 22-23 0.1681813723492951 1109 24-25 0.328015904133629 1109 26-27 0.25946938911743445 1109 28-29 0.2720598321797638 1109 30-31 0.3573984801675607 1109 32-33 0.3065248849114255 1109 34-35 0.24153371510057298 1109 36-37 0.24432535223379404 1109 38-39 0.26760055646143144 1109 40-41 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2316 60-61 -0.9044072047704717 2316 62-63 -1.4476541667036358 2316 64-65 -1.1455018464275355 2316 66-67 -0.9857175499452069 2316 68-69 -0.6914747530425771 2316 70-71 -0.7450362800041717 2316 72-73 -0.8649541814238049 2316 74-75 -0.7680749544349084 2316 76-77 -0.7080919252087696 2316 78-79 -0.7449143806096359 2316 80-81 -0.7734188926664203 2316 82-83 -0.6908449438325235 2316 84-85 -0.6832380819038093 2316 86-87 -0.5836934563389917 2316 88-89 -0.6048133774683109 2316 90-91 -0.42788523266356293 2316 92-93 -0.42611483323079113 2316 94-95 -0.6865781501267776 2316 96-97 -0.5456266193105108 2316 98-99 -0.4570780489577295 2316 100-101 -0.39090252947503146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2236.0 3 127.0 4 62.0 5 57.0 6 351.0 7 1750.0 8 1439.0 9 720.0 10 723.0 11 1277.0 12 2331.0 13 3299.0 14 3524.0 15 3493.0 16 3810.0 17 4156.0 18 4627.0 19 5204.0 20 6256.0 21 7569.0 22 8443.0 23 8979.0 24 8653.0 25 8372.0 26 8520.0 27 9454.0 28 10718.0 29 12337.0 30 14497.0 31 17741.0 32 22591.0 33 30208.0 34 44553.0 35 73014.0 36 146263.0 37 332762.0 38 505794.0 39 161553.0 40 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.016854919857536 19.579756726260385 13.859492350793486 23.543896003088587 2 14.571682532789609 20.31388461042618 41.64385699455146 23.47057586223275 3 17.760962296134338 26.448078977119273 30.401210063231098 25.389748663515288 4 11.776369226509926 17.386002685762406 40.253322087240456 30.58430600048721 5 12.83194697669306 38.50947944960092 35.76040174600191 12.898171827704108 6 29.831100425233988 38.58862750099728 18.978339389367253 12.601932684401485 7 26.98832258222091 32.862174990298215 23.10842386068512 17.041078566795754 8 25.59746042428934 36.28366555920939 20.50304386792741 17.615830148573863 9 26.291744854526485 16.23152833012441 20.393022047280418 37.083704768068685 10-11 24.292066106619682 26.590269076275252 29.467729231779533 19.64993558532553 12-13 25.237244433257604 23.90803108237059 29.243948846453538 21.610775637918266 14-15 22.37457715202987 25.42661340331385 26.603633541257615 25.59517590339866 16-17 22.26503656472935 28.495399275859345 27.052203699227 22.187360460184298 18-19 22.04109322460847 27.866758250140865 28.54180213765593 21.550346387594736 20-21 23.054974074909023 26.885582123806508 28.4434605886072 21.615983212677275 22-23 22.670048420567213 26.940043944716464 28.092643328947904 22.297264305768422 24-25 22.217934458249687 27.088329444956866 28.326901687374534 22.366834409418914 26-27 22.36917378826563 27.241578449947152 28.069482344730716 22.3197654170565 28-29 22.374533432939984 27.19915983170862 28.121222150520204 22.305084584831192 30-31 22.577300010173285 27.13089626640442 28.011258435348772 22.28054528807352 32-33 22.43481539704166 27.174148193711545 28.01596833730858 22.37506807193822 34-35 22.61705094575476 27.242808985373696 28.02004645455966 22.120093614311884 36-37 22.66309192531005 27.118118990079076 27.947585524331174 22.271203560279698 38-39 22.552555128343784 27.277677067904072 27.867685872436997 22.30208193131515 40-41 22.719083229915572 27.121849458764363 27.908253839225576 22.250813472094492 42-43 22.448029170707137 27.01164638719992 28.02376119228961 22.51656324980333 44-45 22.623653392292393 27.248412944785528 27.781464225342184 22.346469437579895 46-47 22.627853994253204 27.24630352541964 27.61637463845282 22.509467841874333 48-49 22.570776755594675 27.238251663566 27.699911775840814 22.49105980499851 50-51 22.681621013000942 27.462427153994607 27.40575691041424 22.45019492259021 52-53 22.77293605193132 27.43085710411688 27.341028118167486 22.455178725784307 54-55 22.581479582980755 27.361158573629286 27.615557671027798 22.44180417236217 56-57 22.518593050684157 27.462693549775945 27.459371243335895 22.559342156204 58-59 22.651794711138777 27.430085127908853 27.51142249183236 22.40669766912001 60-61 22.71916346578707 27.49703366883367 27.505952160937074 22.27785070444219 62-63 22.62259832260195 27.399524453550477 27.539143751934684 22.43873347191289 64-65 22.514322290698598 27.320841687579122 27.567248147035627 22.597587874686653 66-67 22.491906478173167 27.506991901224293 27.404219173011718 22.596882447590826 68-69 22.626424619706015 27.391673320653474 27.514291950280146 22.467610109360365 70-71 22.55526273419824 27.159250565634824 27.542882322534723 22.742604377632215 72-73 22.532409314086888 27.22751855410987 27.542281676725462 22.697790455077786 74-75 22.498618077245244 27.40703814106803 27.43735566113558 22.65698812055114 76-77 22.46283729251754 27.278051248523187 27.47049432267498 22.788617136284287 78-79 22.43336980462197 27.379412421085338 27.346140566632233 22.84107720766046 80-81 22.500063586804465 27.4588169463073 27.321639013471927 22.719480453416306 82-83 22.611302751895344 27.36221193405869 27.44604253114144 22.580442782904527 84-85 22.60682747795906 27.44659431413099 27.366131024616745 22.580447183293202 86-87 22.544127443684854 27.353811156746715 27.42466594003787 22.677395459530562 88-89 22.529501574908846 27.3552785547991 27.352702294092364 22.762517576199688 90-91 22.47207538434581 27.4575475007397 27.451175770615006 22.61920134429949 92-93 22.501453150922647 27.301714802820555 27.51774708225936 22.679084963997447 94-95 22.40393356033034 27.413955909796822 27.50048637373975 22.681624156133086 96-97 22.380141374667275 27.1527906784384 27.617858552109702 22.849209394784623 98-99 22.336845473515226 27.17561966382703 27.502247344878967 22.98528751777877 100-101 22.51998479673774 27.106969054715808 27.561507265796354 22.811538882750096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2074.0 1 1086.5 2 92.5 3 81.5 4 68.5 5 66.5 6 79.0 7 122.5 8 220.0 9 288.0 10 291.0 11 294.5 12 350.0 13 411.5 14 446.5 15 480.5 16 540.0 17 631.0 18 714.0 19 778.5 20 909.0 21 1064.5 22 1257.0 23 1693.0 24 2361.0 25 3280.0 26 4638.5 27 6667.0 28 9418.5 29 12866.0 30 16944.0 31 21556.5 32 26436.0 33 30868.5 34 34710.0 35 37949.0 36 41700.5 37 45052.5 38 47649.0 39 50187.0 40 51768.0 41 52981.5 42 53720.0 43 55053.0 44 56316.5 45 57014.5 46 57716.5 47 57379.0 48 56432.0 49 55265.0 50 53491.0 51 51143.5 52 49164.5 53 47968.0 54 46391.0 55 43213.0 56 39003.5 57 34962.5 58 31289.5 59 27006.5 60 21937.0 61 17660.0 62 14111.0 63 10610.0 64 7619.5 65 5625.5 66 4348.0 67 3225.0 68 2396.5 69 1827.0 70 1384.5 71 1076.5 72 794.0 73 589.5 74 451.0 75 323.0 76 275.5 77 198.0 78 126.0 79 90.0 80 74.5 81 60.5 82 47.0 83 39.0 84 28.5 85 19.0 86 14.0 87 12.0 88 8.5 89 6.5 90 5.0 91 2.5 92 4.0 93 6.0 94 3.5 95 1.5 96 1.5 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2542688332870862 2 0.255825443126214 3 0.26584188904755784 4 0.26211956117138274 5 0.2573820529653418 6 0.2408007742441982 7 0.24459078080903107 8 0.24519988900695058 9 0.24066541686688278 10-11 0.23870273489580868 12-13 0.24469229884201762 14-15 0.24607971195950107 16-17 0.19332417415080164 18-19 0.18794371840251223 20-21 0.1791793282213364 22-23 0.20235927908660842 24-25 0.20912714795238127 26-27 0.21139438402241517 28-29 0.21004081024926063 30-31 0.2111575086121131 32-33 0.2048633905669444 34-35 0.1891957741426802 36-37 0.1627672462218372 38-39 0.18689469872831746 40-41 0.17362967575140265 42-43 0.16185358392495786 44-45 0.14862240029237195 46-47 0.15654080686532618 48-49 0.159078757689991 50-51 0.16033081343015898 52-53 0.1722422626339192 54-55 0.18422139052633715 56-57 0.1821233511779476 58-59 0.19274890529721098 60-61 0.21017616762657607 62-63 0.19257970857556664 64-65 0.18753764627056588 66-67 0.16720020032891844 68-69 0.15322455112109748 70-71 0.16632037737636796 72-73 0.16868913147938847 74-75 0.2148798364882882 76-77 0.2103453643482204 78-79 0.22679128569204843 80-81 0.21707939386966438 82-83 0.21440608566768413 84-85 0.2019870462989909 86-87 0.18425522987066603 88-89 0.17352815771841604 90-91 0.15589785932307776 92-93 0.15752214785086324 94-95 0.16019545605284352 96-97 0.17758887903787976 98-99 0.1689260068896905 100-101 0.1953545348105335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477570.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.046794545153624 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65141680998029 40.30436859082959 2 15.789282670714854 18.014559290632494 3 5.8637241696656215 10.035200039290986 4 2.8622028365115986 6.531179886441324 5 1.5535778381969967 4.431331787276399 6 0.9089115193263015 3.1110293221638567 7 0.575828477902313 2.299441821049925 8 0.38945803759692116 1.777386612380022 9 0.2863943713836304 1.4704092776889348 >10 1.0815146275114813 9.859801486906074 >50 0.028166180488451154 1.0986379543634857 >100 0.00916539711875141 0.7757119437879949 >500 1.1902120095521303E-4 0.06151538865652988 >1k 2.3804240191042605E-4 0.22942659853246633 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1960 0.132650229769148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.767868865772856E-5 7 0.0 0.0 0.0 0.0 6.767868865772856E-5 8 0.0 0.0 0.0 0.0 6.767868865772856E-5 9 0.0 0.0 0.0 0.0 6.767868865772856E-5 10-11 0.0 0.0 0.0 0.0 6.767868865772856E-5 12-13 0.0 0.0 0.0 0.0 6.767868865772856E-5 14-15 0.0 0.0 0.0 0.0 2.030360659731857E-4 16-17 0.0 0.0 0.0 3.383934432886428E-5 2.7071475463091426E-4 18-19 0.0 0.0 0.0 1.0151803298659285E-4 3.3839344328864286E-4 20-21 0.0 0.0 0.0 1.3535737731545713E-4 3.3839344328864286E-4 22-23 0.0 0.0 0.0 2.3687541030204998E-4 4.060721319463714E-4 24-25 0.0 0.0 0.0 4.399114762752357E-4 4.7375082060409997E-4 26-27 0.0 0.0 0.0 6.429475422484213E-4 4.7375082060409997E-4 28-29 0.0 0.0 0.0 8.798229525504714E-4 4.7375082060409997E-4 30-31 0.0 0.0 0.0 0.0016242885277854857 4.7375082060409997E-4 32-33 0.0 0.0 0.0 0.0035192918102018855 4.7375082060409997E-4 34-35 0.0 0.0 0.0 0.005786527880235792 4.7375082060409997E-4 36-37 0.0 0.0 0.0 0.010253321331645877 4.7375082060409997E-4 38-39 0.0 0.0 0.0 0.019931373809701065 4.7375082060409997E-4 40-41 0.0 0.0 0.0 0.0326888066216829 4.7375082060409997E-4 42-43 0.0 0.0 0.0 0.04439721975946994 4.7375082060409997E-4 44-45 0.0 0.0 0.0 0.06094465913628457 4.7375082060409997E-4 46-47 0.0 0.0 0.0 0.07955629851715992 4.7375082060409997E-4 48-49 0.0 0.0 0.0 0.09914927888357236 4.7375082060409997E-4 50-51 0.0 0.0 0.0 0.12422423303126079 4.7375082060409997E-4 52-53 0.0 0.0 0.0 0.14960374127790899 5.075901649329642E-4 54-55 0.0 0.0 0.0 0.17931468559865182 5.414295092618285E-4 56-57 0.0 0.0 0.0 0.21180045615436155 5.414295092618285E-4 58-59 0.0 0.0 0.0 0.2533551709902069 6.091081979195572E-4 60-61 0.0 0.0 0.0 0.3018131120691405 6.767868865772857E-4 62-63 0.0 0.0 0.0 0.3539257023355915 6.767868865772857E-4 64-65 0.0 0.0 0.0 0.4117571417936206 6.767868865772857E-4 66-67 0.0 0.0 0.0 0.4749690369999391 6.767868865772857E-4 68-69 0.0 0.0 0.0 0.5473513945193798 6.767868865772857E-4 70-71 0.0 0.0 0.0 0.636551906170266 6.767868865772857E-4 72-73 0.0 0.0 0.0 0.734043057181724 8.121442638927428E-4 74-75 0.0 0.0 0.0 0.8342413557394912 8.121442638927428E-4 76-77 0.0 0.0 0.0 0.9405645756207828 8.121442638927428E-4 78-79 0.0 0.0 0.0 1.0647888086520436 8.121442638927428E-4 80-81 0.0 0.0 0.0 1.2056619990931057 8.798229525504714E-4 82-83 0.0 0.0 0.0 1.3667034387541706 9.475016412081999E-4 84-85 0.0 0.0 0.0 1.52940300628735 9.475016412081999E-4 86-87 0.0 0.0 0.0 1.7038448263026456 9.475016412081999E-4 88-89 0.0 0.0 0.0 1.895713908647306 9.475016412081999E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1575 0.0 54.308964 1 GTATCAA 3020 0.0 41.540966 1 TCAACGC 3550 0.0 34.534744 4 ATCAACG 3685 0.0 33.27069 3 CAACGCA 3730 0.0 32.613396 5 AACGCAG 3910 0.0 31.598137 6 TATCAAC 4040 0.0 30.582401 2 TATACCG 160 1.899025E-9 29.699211 5 GTATACG 85 6.392019E-4 27.953144 1 ACGCAGA 4435 0.0 27.643366 7 GTACATG 5115 0.0 27.220844 1 TACATGG 5065 0.0 26.457527 2 CGCAGAG 4650 0.0 26.262152 8 ACATGGG 5165 0.0 25.209246 3 TACCGTA 125 2.2503073E-4 22.808994 7 GCAGAGT 5545 0.0 21.68049 9 AGAGTAC 4935 0.0 20.79777 10-11 CATGGGG 3500 0.0 20.772476 4 GTACCGT 145 6.1308657E-4 19.662926 6 GTGGTAT 945 0.0 19.108711 1 >>END_MODULE