FastQCFastQC Report
Fri 10 Feb 2017
SRR2568573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477570
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.14341114126572685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.11755788219847453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.057.4253961
GTATCAA30250.044.30381
TCAACGC35400.037.7076234
ATCAACG35850.037.244393
CAACGCA37200.035.883065
TATCAAC38600.034.5933072
AACGCAG39200.034.2888576
ACGCAGA45350.029.2199527
GTACATG48100.029.0482161
CGCAGAG46650.028.3038648
TACATGG50150.027.668462
ACATGGG49150.027.5527553
TATACCG1151.2813443E-424.7844625
CATGGGG33250.023.144594
GCAGAGT56100.023.1127939
AGAGTAC48750.019.97247310-11
GAGTACT29950.019.82283612-13
CAGAGTA54100.019.27036310-11
CGTATAC1250.00601402919.0097851
ATACCGT1250.00603523218.9982056
Produced by FastQC (version 0.11.5)