FastQCFastQC Report
Fri 10 Feb 2017
SRR2568570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685747
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29900.030.675941
GTACATG79550.029.8704381
ACATGGG79350.028.927573
GGTATCA20700.028.4684031
TCAACGC30200.028.0086634
TACATGG83800.027.901842
ATCAACG30950.027.331983
CAACGCA31700.026.9816345
AACGCAG32700.026.1550456
TATCAAC35200.024.3019772
CATGGGG48250.024.1294864
GAGTACA54700.022.3283961
GTATTAC5700.020.8438011
AGTACAT56650.020.3014582
ACGCAGA43500.019.1166557
GTATAGA9200.019.112861
CGCAGAG44750.018.6885098
TATAAGC11900.017.971212
CATGGGA36200.017.7216644
TAATACT21350.017.3610524
Produced by FastQC (version 0.11.5)