FastQCFastQC Report
Fri 10 Feb 2017
SRR2568570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685747
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.036.4464951
GTATCAA29350.036.105961
ATCAACG30000.032.778853
GTACATG80650.031.876821
TCAACGC30750.031.8189494
CAACGCA32900.029.8839635
AACGCAG33150.029.5114676
ACATGGG82700.029.0088443
TACATGG87050.028.7641242
TATCAAC36650.027.3538062
CATGGGG49450.024.7809054
GGATACG1252.25037E-422.8098981
ACGCAGA42350.022.5397837
GAGTACA55550.021.8996931
ATATAAG10650.021.8639491
CGCAGAG44050.021.3464878
TAATACT19900.021.0035784
AGTACAT55550.020.0998632
GTATAGT8850.018.2565121
TCTATAC8300.017.7430483
Produced by FastQC (version 0.11.5)