FastQCFastQC Report
Fri 10 Feb 2017
SRR2568569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150583
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.042.6249851
GTATCAA33800.042.181431
TCAACGC38650.035.7816434
CAACGCA39250.035.2330175
ATCAACG39800.034.747753
TATCAAC42650.032.7600862
AACGCAG42900.032.676916
ACGCAGA50150.027.6693087
CGCAGAG50950.026.9550468
GTACATG69700.026.4554841
TACATGG71350.024.4449332
ACATGGG70850.023.8795933
GCAGAGT62950.021.439229
CATGGGG46650.020.8842544
GTATAGG8700.020.7577881
GTACCGT2702.2904715E-819.3599956
GTGTAGC15500.019.3163741
AGAGTAC53450.019.02621810-11
GTATATA13100.018.5018711
GTATAAG8600.017.6835041
Produced by FastQC (version 0.11.5)