FastQCFastQC Report
Fri 10 Feb 2017
SRR2568569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150583
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.044.285421
GTATCAA35150.038.9289551
TCAACGC38800.034.1559944
ATCAACG39850.033.3767783
CAACGCA39900.033.0953035
TATCAAC43650.030.7990322
AACGCAG44550.029.9579936
GTACATG69100.026.6783661
TACATGG70850.025.1435322
ACGCAGA52750.025.1209357
ACATGGG69050.025.1098353
CGCAGAG54000.024.4514778
CATGGGG43900.022.722144
ATACCGT2205.6505087E-821.5889436
GTATAGC11000.020.7326161
TAGCCCT20150.019.8015274
TATACCG1200.0047647719.7917045
GCAGAGT65900.019.6036789
GTGTAGC18100.019.4248961
GTATTAC5900.018.5217251
Produced by FastQC (version 0.11.5)