FastQCFastQC Report
Fri 10 Feb 2017
SRR2568565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.040.386571
GTATCAA31750.036.231971
TCAACGC32800.033.4765134
CAACGCA33200.033.0697065
ATCAACG35300.030.97233
AACGCAG36750.029.7433856
TATCAAC39350.028.3885652
GTACATG72350.026.9380171
ACGCAGA43550.024.88257
ACATGGG72150.024.6408443
TACATGG76450.024.4984742
CGCAGAG44550.024.2172858
CATGGGG49400.020.8802094
TATAGCG1604.963325E-520.7968882
GTATATA15050.019.5828321
CGTATAC2001.2773653E-519.01433
GTACCGA2302.0495045E-618.5966456
GCAGAGT57900.018.3043949
GTATAGC11850.018.051551
CCCTATA5350.017.7703742
Produced by FastQC (version 0.11.5)